XB-CLONE-3456124
Clone Name: IMAGE:7751738
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Gene:
acnat1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DN052190 [+]
tctttaaactgctcttcctttttgaactgacagaatccgattgctgcctccgccacctaaaggatctttcagcagcctcagttggtgcctccatgtcttt
cattgatcttccttttgaattgctgctgtgctcccattgggtgttcccttatttataaaatgcttccttactggagtaacagaggtcttatccaactact
gggacttctctacaagtcgtcaacctcttcaaaccaccctggtgcccctggacaatgctttatggatcttcatcttcaaactgccagtgtgccccagttg
gcccttattattcttcagaatgcttccgtagaactcctatagaactactcttaaaatcttcttaactgctaggctttctctttaagttgtcctctctaca
acctcaggtgggacctcaagcttccaaatatcttccacatagaccttcagagaccccagagatctcagagactacacagagactccaatagcccccacaa
gtccaataaatagtggcattatacagcagcccctctggaatttgcctgaacccacagattgccggttcagtcctgcacagctgctttggtgtttggggca
ctaatcaaaatcatgtgattcattctaaacccaaatcatatgatttcccaaggtctgagattcagaactaatgttattaatattattgtgggaaaagagt
tattgaaaagtagaaagggaaatttggaaaactggtaagatctgcaaagttctactgattttctaacctcaaccctttaacgctttgcctgtgttgtatt
ctatacgtgctggcaggcatttatgctttaact
3' EST: DN052189 [+]acttgcacacttcaggtttcagggccaaaaattccaataataataggtttaccctagcaaactatacatttttggaaagaacagattctgataaatccaa
aattagtaaatatgtctttctacttcaaagttcccagtctcaatgctttcctaaacatcagtttttattataatcttctgaaaaataatctcaaagcttc
cagtttacattatcctatttcctaaaggatgccagtgtgtggcatctaaaaagtatgtttactcaagaaagtcatatatttataagtacaaattctcctg
aatccaaaatgggtacccatatatttctactccaaagcaccaaaatgcaaagctttcttaaaaaccttttaagccgccaagaaatccctgagatgaacat
aattcttggcagttaaagcataaatgcctgccagcacgtatagaatacaacacaggcaaagcgttaaagggttgaggttagaaaatcagtagaactttgc
agatcttaccagttttccaaatttccctttctacttttcaataactcttttcccacaataatattaataacattagttctgaatctcagaccttgggaaa
tcatatgatttgggtttagaatgaatcacatgattttgattagtgccccaaacaccaaagcagctgtgcaggactgaaccggcaatctgtgggttcaggc
aaattccagaggggctgctgtataatgccactatttattggacttgtgggggctattggagtctctgtgtagtctctgagatctctggggtctctgaagg
tctatgtgga
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Primers for sequencing: t3 or t7 for 3' end, sp6 for 5' end. Library constructed by Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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