XB-CLONE-3455807
Clone Name: IMAGE:7752439
|
Gene:
supt20h
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DN052404 [+]
catttcaactnttttgaaatactgtcatttggggtacttgcatattagaagactaggaataatatttttgtaacaattatggcttgcatctaaagtggac
attgatttactttggtataaatgcaagtacaggtatcagaccccttatctggaaaacgcatattccagaaagttccaaattacagaaaggttatctccca
cagactccattttaatcaaataattcacatttttaaaaatgatttcctttttctctgtaataataaaacagtgccttgtacttgagcccaactaaaatta
aaataattaattcttatttgagccaaaacaatcctattgggtttaattatggtttaaatatttttttttagcatacttaaggtaggagatccaaattacg
gaaagaccccttatccagaaaaccccaggtcctgagcattctgaacaacaggtccaatacctgtactgagatctcttacttcaaaatgaagacagaagcg
gtgttaaacatcagtaaatattttacaatattgccatctactggtctacatactgtagtttgtttaaacaattgtgtaacaatacatttacaaggtatta
ccaaatgtatgtttcagttaaaaaaagaggtcagtttgtttatccaaactgatgataataataaggataagtcaaatacatatacaaaacttaataaagg
gttaaaaaatagccctgatagagccataacggtatggctactagcaatttaaagcgtactatcagaattacccaacttaaagtatttgcatatgtgttta
tcagcccaaaaaagacctggtgcgcctactgctg
3' EST: DN052403 [+]tgtgtaccaatattactgtaagcaacctgctgctaatttctcatatattaatctaccatgacattatattgtccatttatttaagatgattatcctccaa
tttaagcaacggctcagtgtaaagggcatgaagtgttactcatgttttgttggactaagccaagagaaaatgcatattaaaattgttactttaatgcaaa
ggtttgcccaagctattcttccattgttctattctaaaaatctgttgaatatatcctctgatagggcatacataatttcatggccaaataacatacagtg
gtgtgaaaaactatttgcccccttcctgatttcttattcttttgcatgtttgtcacacttaaatgtttctgctcatcaaacacatttaactattagtcaa
agataacacaagtaaacacaaaatgcagtttttaaatgagggtttttattattaaggaagaaaaaaaatccaaacctacatggccctgtgtgaaaaagtt
attgccccctgaacctaataactggttgggccacccttagcagcaataactgcaatcaagcgtttgcgataacttgcaacgagtctttttcagcgctctg
gaggaattttggcccactcatctttgcagaattgttttaattcagctttatttgagggttttctagcatgaaccgtctttttaaggtcatgccacaacat
ctcaataggattcaggtcaggactttgactaggccactccaaagtcttcattttgtttttcttcagccattcagaggtggatttgctggtgtg
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Primers for sequencing: t3 or t7 for 3' end, sp6 for 5' end. Library constructed by Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: