XB-CLONE-3454795
Sequence:
Full length sequence: BC155497 [+]
CAAAATAGAGAAGTCGGTGTGTGCAAACCCTTCCTTACAGCTGCTTTTGTTCCATCTACCTGGCTTATCTGGCCAGATCAGCTGACGTGTTTGGGAAGTA
TGAGGGTATTTTTAAAAGAGCTACGGTATGTAGAGCACAGACAGGATCTCTGAAACATGGCTGAGGATACAGTACTCCTTGCTGCCGTTTCCATTCTTTC
TGCTTGTCAGCTTGCATATTATTCAGCGCTTGTGGGCAAGTCAAGGGTAAAGCATAAAGTCAATCCCCCTGCAGTGACGGGGCCACCAGAATTTGAGAGA
ACCTTGCGTGCCCAACAAAACTATGTGGAGTTCATACCAATATTCTTGGTACTGCTGTGGACTGCTGGCATTTACTTTCATCAAGAAACAGCTGCAGTTT
TTGGATTGCTACACATAATAGCACGGCATATCTATTACAGAGGGTATACGAATTCTGTACAAGGCAGGGTTCCTGGATTCTTGATGTGTTTGATAATGCT
GTTTCTGCTGCTTCTTGTGGCTGCTGTTGGCATTGCAGACAGTCTTCTGAAGAAATATCTGGACCTGAACATTTTGAAAAAAATGCACAAGATAATATAA
TAATTAGGAAGGAAATCAAATTGCATTTTGGCTGCTTTATGTGTAACTGAACTGGTGCAGTACAGCAGTTTAGCATGATAATATCAAGCCCCTGAAGTCT
TCATCTCAATGACATGTCTATAATGATATACAATAGCATACAATGTAGAGAGTGTGAGTTTGAGTTTCATTTTTGAGTCTGAGATAAAAAATTAATTTGG
AATGAAAAAAAGTTGATAATGAAGAAAAATTGTATAGTTCCATTTATTTCAATAAGACTATAAAATGATGATGGTTTTAAAACTCTCAATATAAAAAATC
TCAAACATCAAAAAAAAAAAAAAAAAA
5' EST: DN053084 [+]caaaatagagaagtcggtgtgtgcaaacccttccttacgctgcttttgttccatctacctggcttatctggccagatcagctgacgtgtttgggaagtat
gagggtatttttaaaagagctacggtatgtagagcacagacaggatctctgaaacatggctgaggatacagtactccttgctgccgtttccattctttct
gcttgtcagcttgcatattattcagcgcttgtgggcaagtcaagggtaaagcataaagtcaatccccctgcagtgacggggccaccagaatttgagagaa
ccttgcgtgcccaacaaaactatgtggagttcataccaatattcttggtactgctgtggactgctggcatttactttcatcaagaaacagctgcagtttt
tggattgctacacataatagcacggcatatctattacagagggtatacgaattctgtacaaggcagggttcctggattcttgatgtgtttgataatgctg
tttctgctgcttcttgtggctgctgttggcattgcagacagtcttctgaagaaatatctggacctgaacattttgaaaaaaatgcacaagataatataat
aattaggaaggaaatcaaattgcattttggctgctttatgtgtaactgaactggtgcagtacagcagtttagcatgataatatcaagcccctgaagtctt
catctcaatgacatgtctataatgatatacaatagcatacaatgtagagagtgtgagtttgagtttcatttttgagtctgagataaaaaattaatttgga
atgaaaaaagttgataatgaagaaaattgtatagttccatttatttcataagactataaaatgatgatggttttaaaactctcaatataaaa
3' EST: DN053083 [+]gatgtttgagattttttatattgagagttttaaaaccatcatcattttatagtcttattgaaataaatggaactatacaatttttcttcattatcaactt
tttttcattccaaattaattttttatctcagactcaaaaatgaaactcaaactcacactctctacattgtatgctattgtatatcattatagacatgtca
ttgagatgaagacttcaggggcttgatattatcatgctaaactgctgtactgcaccagttcagttacacataaagcagccaaaatgcaatttgatttcct
tcctaattattatattatcttgtgcatttttttcaaaatgttcaggtccagatatttcttcagaagactgtctgcaatgccaacagcagccacaagaagc
agcagaaacagcattatcaaacacatcaagaatccaggaaccctgccttgtacagaattcgtataccctctgtaatagatatgccgtgctattatgtgta
gcaatccaaaaactgcagctgtttcttgatgaaagtaaatgccagcagtccacagcagtaccaagaatattggtatgaactccacatagttttgttgggc
acgcaaggttctctcaaattctggtggccccgtcactgcagggggattgactttatgctttacccttgacttgcccacaagcgctgaataatatgcaagc
tgacaagcagaaagaatggaaacggcagcaaggagtactgtatcctcagccatgtttcagagatcctgtctgtgctctacataccgtagctcttttaaaa
taccctcatactttccaaacacgtcagctgatc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Primers for sequencing: t3 or t7 for 3' end, sp6 for 5' end. Library constructed by Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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