XB-CLONE-3442428
Sequence:
Full length sequence: BC123985 [+]
TGATGGAGCGCTACAGGCACTTACACAGGATCATTCTCATGGTTGTTCTGCAAGGGGGGTTCCCAGGTTCGTACAGTCAGGATCGGGCCATTACCCTCCC
TGAATCCATCAGAGTATTGAGAGGATCCTGTGTGGAGATTCCCTGCACATTTACCGACCCCATGGGCTTTGAAAACTCCAATCTGATCTGGTACAAGAAA
TCATTATCAACTTATGAAATTATTTTTGACCAACATTATCCATCTCAAATAAATGAACGTTACAGAGGCCGCACTTCCCTGGTCGGCAATGAGCCAAACA
GCTGCTCCCTGAGGATTAATGATGTGCAAGAAAGTGGCACTTATTATCCCTATATATATGGGAACTGTGATGATATCACTGATTGTTACACTGTGAGCAT
CTCCGTTACAGATGTTCCCAGTAGGCCAGCAATACAGATACCCACAGATCTAACTGAGGGGAAACGTGCCCCCATCTCCTGCTCTGTAGAACACACGTGC
CCCCCCCAGCCCCCCTACTCTACAGTGGAACAGAGCTGGGTATAAACTCACTGACAGGCAGCAGAGCCTTAAGGATGGAGTATGGAAAGCTGAGACTGTA
ATGGAATATCTCCCCTCCTACCGGGATCATGGGGCTGAACTTGAATGTAAAGCTACGTATCCAAATGGGCTCAGATCCCAACGCCGGGTCACTTTAAACA
TAACATTCTCTCCAAAAAACACAAGAATTGTTATATCTGATGGGAAGAAGGAAAGGAAAGAAGGTGATGCCGTGAGTTTAACCTGTGAAAGTGATGCCAA
CCCTGCTGCCAATGCCTTTACCTGGTACAACAATAAGAGAGATGGAACTGTAGAGCTGAGGGAGGAGCAGGGGCGGAGCATAACTGTGACTGTGGGGCGG
GGCAGAGAGACATTCTCCTGCACTGCAAGGAACCCACTGGGAACTGAAAACTCCACTCTATCTGAGCTGCAGGTTTTATTCTCTCCAAAAAACACAAGAG
TTCTTATACCTGATGGGAAGAAGGAAAGGAAAGAAGGTGATGCAGTGAGTTTAACTTGTGCCAGTGATGCCAACCCTGCTGCCAATGCCTTTACCTGGTA
CAACAATAAGAGAGATGGAACTGTAGAGCTGAGGGAGGAGCAGGGGCGGAGCATAACTGTGACTGTGGGGCGGGGCAGAGAGACATTCTCCTGCGCTGCA
AGGAACATAATTGGAGATGGAAAGTCTACTGTTTTGGGTCTGCAGGTTTTATATAAAGCCAAAGACGTCATAATAGAAGGAAAAGATGAAGTGAAGGAAG
GAGCAGCCCTGGAGCTTGAATGCCATTTCTCAGACTATAACCCACCAACAACTCAGTACAGCTACAGCTGGTATCTAAATGGGAATCCTGTGAATGGGGA
GACTGGCAGAATATTACAGATAAATAACATTACAGAGTCCCATTCTGGCAACTACAGCTGCAATGTGCAGAACGGAGCGGGACCTTCCTCTTCCCCAGGC
TTTGCTGTTACACTGACGTCTGCAACGACTGGAGAGGAACAGCTCACAACAGTCATATCCGGAATATCCATAATATCCGGAAGTGTAGCTGGTGCTGTAT
TGATCATACTATTGATTCTGGCCCTATATTTATATCTAAGATACAGAAAGTCACAGAAAGGGGGAGCTTCAGGGGGCAGAAGAGTGGAGCCAGATTATCA
GAATTCCCAGCCCACTGAGAGTCCCTACGCTGCCCTTCAGATGGGAGACACGGCCGTGTACGATGTAATGAAGAATCCCCAGGCCACTGAGAGTCCCTAC
GCTGCCCTTCAGATGGGAGACACGGCCGTGTACGATGTAATGAAGAATTCCCATTCCACCGAAAATCCCTACGCTGCCCTTCAGCTGAGAGACGCGGCCC
TGTACCATGATATCAAGCTGAAAAATTCAGAAAGACTTGATATTAATCCCAAGTGAGAAAAAGGAACACGTGGGATGTGCTGGGTCGTACCAACGAGCCT
ATGATGTCCCTTGTTGGTCACCTGTAGAAAGAAGAATAGGCTTACTAACAGTCACTGAACTATAAACATAAAATACCTAGGTCCTGTCAGGACCTGTCCA
ATAAGTTAACTTTTAGTATGTTATAGAATGGCCAATTCTAAGCAACTTTTCAATTGATTTTCATTACTTATTTCTTATAGTTTTTAAACTATTTGCAGCT
TTCAAATGGGGGTCACTGACCCCGGCAGCCAAAAAACTATTCCTCTGTGAGGCTACAGTTTTATTGTTTTTGTTACTTTTTATTACTTATTCTTTCTATT
CAAATCCTCTCTTATTCATACATCAGTCTTTCATCCAAACGACACCCTGGTTGCTAAGGTAATTTGAATCCTAGCAACCAAATAACTGCTGAAACGCCAA
ACAGGAGAGCTGCTGAACAAAAACTGAAATAATCTGAAAAAACTATAAATATTAAAAAAAGAAGACTAACTTGAAAAAAAAAAAAAAAAAA
5' EST: DN061599 [+]tgatggagcgctacaggcacttacacaggatcattctcatggttgttctgcaaggggggttcccaggttcgtacagtcaggatcgggccattaccctccc
tgaatccatcagagtattgagaggatcctgtgtggagattccctgcacatttaccgaccccatgggctttgaaaactccaatctgatctggtacaagaaa
tcattatcaacttatgaaattatttttgaccaacattatccatctcaaataaatgaacgttacagaggccgcacttccctggtcggcaatgagccaaaca
gctgctccctgaggattaatgatgtgcaagaaagtggcacttattatccctatatatatgggaactgtgatgatatcactgattgttacactgtgagcat
ctccgttacagatgttcccagtaggccagcaatacagatacccacagatctaactgaggggaaacgtgcccccatctcctgctctgtagaacacacgtgc
cccccccagcccccctactctacagtggaacagagctgggtataaactcactgacaggcagcagagccttaaggatggagtatggaaagctgagactgta
atggaatatctcccctcctaccgggatcatggggctgaacttgaatgtaaagctacgtatccaaatgggctcagatcccaacgccgggtcactttaaaca
taacattctctccaaaaacacaagaattgttatatctgatgggaagaa
3' EST: DN061598 [+]caagttagtcttctttttttaatatttatagttttttcagattatttcagtttttgttcagcagctctcctgtttggcgtttcagcagttatttggttgc
taggattcaaattaccttagcaaccagggtgtcgtttggatgaaagactgatgtatgaataagagaggatttgaatagaaagaataagtaataaaaagta
acaaaaacaataaaactgtagcctcacagaggaatagttttttggctgccggggtcagtgacccccatttgaaagctgcaaatagtttaaaaactataag
aaataagtaatgaaaatcaattgaaaagttgcttagaattggccattctataacatactaaaagttaacttattggacaggtcctgacaggacctaggta
ttttatgtttatagttcagtgactgttagtaagcctattcttctttctacaggtgaccaacaagggacatcataggctcgttggtacgacccagcacatc
ccacgtgttcctttttctcacttgggattaatatcaagtctttctgaatttttcagcttgatatcatggtacagggccgcgtctctcagctgaagggcag
cgtagggattttcggtggaatgggaattcttcattacatcgtacacggccgtgtctcccatctgaagggcagcgtagggactctcagtggcctggggatt
cttcattacatcgtacacggccgtgtctcccatctgaagggcagcgtagggactctcagtgggctgggaattctgataatctggctccactcttctgccc
cctgaagc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1905 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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