XB-CLONE-3438042
Clone Name: IMAGE:7808391
|
Gene:
sypl1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DN065385
[+]
tgatagctgtagtcgtgtcttggcctatcgtgttcgtcttcatcctgtttgtgtctccttgcagaaaatggtcggctggcagctgaaccttggcccgcta
aaggagcccctgggctttatcaaattattggaatggtttgcttcaatcttttcatttgccacttgtggtggttacagtggcaaaactgaattaatagtaa
gctgtttaggatcttcagaaaataaaactgctaccgcttcatttatgtatccgtttcgattaaatgttcaagtgtttgaagggatacccaaattattctg
taatcaaacatggaaagactttcaccttacaggagatttttctgccccagcccagttctacgtttctattggagtctttgcctttctttactgcatggct
gctcttgtgttttatctaggttttatgcagttgtatcgggactctcaaaaattcccattatgtgacttcttcattacggtcttctttgctttaatgtggc
tggttagttcctcagcttgggctaaaggtttgacagatataaaagcctccaccagccctcaaggtattgcacagtatacctgcccgactggttacaaatg
tctgcctgggcaagagtcacacatgggaagtctgaacatctctgtggcgtttggatttttgaatctgatactgtgggcaggaaatgcttggtttgtatac
aaggagacaagcctacatactcctccacaacaaccaaacccggagcaagcacctggaaatgtttctccatgaattttaagataaatcctatagaaatgcc
aattcacatttaatggcctacaatctggtgttttcatgcccaaggatgcattgtttatgtgttataaacatgtggatacaaggtattcatgcca
3' EST: DN065384
[+]aacacaaaaccaaaacttatctttaatattgcatcagcaggtacagaatagtttttttttatctgattcagaacaataattcatatacatggagaaaaac
agttaggcatataaacagtattgggaaatagggtaaatgtgtgtctagaatgcaaacttgtggcaaaagctacaatagggctgtaattgcttgtacatat
tcagtaacctgcacaaccatctatttccattaacattttcaacagtgctctaaaaaatagtacatgtaaaagagacgaacttggggttttgtgcaataaa
ataagagaaacaaatgttcaaaatatctagaatataaaaaaaagcatttatactggtaagcttttatattaaacttaagcattgcagagagtttgcctat
tatatgacaaaagcttttggtttaagaggaaatcccaatttttatagttacatcagaataggtattccaaattagtacagtacttcatattctgtcattt
ttcaaaacaacatattgaagaaatatttatcagtgtttgcactcctaaaaaccttagtgttttaaagccaataaactgcagtacatcactgttcacaaca
aaacattgttctgtaactggaaaacctgcttgaaataagtcgtaggcctgtttttcagaaagaggaatcctggcatgaataccttgtatccacatgttta
ttaacacataaacaatgcaatccttggcattgaaaacaccagattgtaggccattaaatgtgaattggcatttctataggatttatctttaaattcatgg
agaaacatttccaggtgcttgctccggggtttggttgtgt
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1783 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: lung | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: