XB-CLONE-3434377
Clone Name: IMAGE:7809414
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Gene:
tp53i13
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC155514 [+]
CCAGCCCCTACCCCCTACCCCCTAGTTGGACAACGGGATGGGGCTGCTGAGTTCTGTGGTGTTTTTGCTGATCTTTGTATTCGATAAAAATTGTGGTGAG
GTGTGCGATAACGGCCGGTTGAATATTCATGTAGACCTTCCAGACAAGAGTGCGTATACGTGTCCCAGGGGACACTGGCCTTACCCAGTTCAGGTGTTTC
CAAGCATAACCACCCAATATGAACCAGAGAAATCACACCGCATCTGTATGGACACTGAGATAGCGTATGCAACCCCAATTCCCAACAGCGGTATGCACCG
TCCAAAATGGGCCAGATATGGAGAATATGTGTATTGCCCCCCACAGCGCTGGGTGCACAACCTTGAGCACGGAGGCGTTGTTTTCTTGTACCATCCTTGT
GTTCACCCCAAACTGAAGAAAGCTTTGTCCCATGGAGCCCGCTCATGCATATACAAGCACATAATCACCCCTCACATGAACCTTTCAACTGAACGGCCTC
TTGCTCTGGTCACTTGGGGCTCCACTCTGGAGATGTCCCATATAGATCTCTATGAAGTTAAAAACTGGCTAATGGCCAACATGGGCAGGACTAGAGAGCA
AGAGATGAAAATAGATGGGTTGTATCAGCACTTGCTTATCCAGCCAGCATCAGGGGTGTTTGATGATCACAACAAGAACATTTGCCCAAGGGTTTGCTGC
AAGCAAGTAAAAAGTGGTTTGAAAAGAAGAAGAAGGGACCCCGCATCACTTGGTTCTGCCCTCATATTAACAAGTACTCCTATTTATAGTGCAGAAATAA
TGAACAGTACTGCAGTGTCAGTAGAGCAACCAGCATTAGATGCCAATGAAAGCCAGCCTGTAATCGCACACACAGTATTGGGGACTGCTTTATCATCTTC
AGAATCTGACGTTAAAGTCCATGTAACATTAATGCCTTCTAATGTGTCACGTGAAAAAGATGCCCTTGTGGATCCTGGGGGAAGACATTATATGGAGAGC
AATGCATCTGGTGTACTACCCCTACAGAAAGAACCAACCTTTCTGGATACTTCCAAAGGCTCCTCACCTGTACAAAGCAAGAACACATCCAAAGAGCCAG
GAGGCAGCCAGGTTGTCCCATCACTAACTGTCACGCAAAAAGAATCAGGGGCGTCTAGAACCCACAACAAAAGCATATCAGAAAAAACTACACTTGAGGG
CCAGGACCCTGCAACCACCACCAAACAAAAACAAAATACGGCTGAGTTTCCTGTGGGGCTTTTACCTGCTACTGCTCCCAAACCAACTGCTGAGCCTCCT
GTAGGGCCTATATCTACTACTGATACCAAACCAACTCCTGGGACTCCTGTAGGGCCTATATCTACCACTATTGCTCCCAAACCAACTGCTGAGCCTCCTG
TAGGGCCTATATCTACTACTGCTTCCAAACCAACTGCCGCCATCATAGAAGGCAAACAAGAGTGCAATTGCAAGCATGAACCAACAAAAGAGGCATCAGC
TGCAGCACAGAAGGCTTCTGGTTTGGAACAAATGAAGGCTTCAGATGTTTTTATTTCTACCCCACGAACAGAGGAAGCAGCATGGGCGGCTGCATGCCTA
ACTTTCCTTTTTGTTTTGCTGACTTTGTCTGTTTTGTACACACAAATCTACAAGAGGTTTCGCAAGAGCCAAAGTCTGTATTGGCCGTCAGAAGGAATCG
CCACTGAGACAGAGACCGTGGCTTCTGTTATCAAGAGGAGGCTGATGTCAGGTCATTCCAAAAGGAAGAAGTGGATTAAACACAAGAAAACCCCAGTGCT
GCTCTATGAAAGTTTAAGTGAAAGCTCAGAGTAAAGGGTTCATTTCTGGGAAGGTGCCCATTCTGCACAAAAGCACTTTACGTTATTGGGTGAACTGCAC
TCTGCACTCGAAAAGGGCAACTTAATGGAGAAATCCAGAATGAAGTCTGCAAAAGAAACTATAGAAAACATTGGATTTTATTTCTCTCTATCTCCAAAAA
ATGATCATGCTTCACTTTTAGAGAGATCCCAGGGATCTACAGCTGTTTATAAAAGAATGACGGCAGCTTTAACAGAAGCCAATGGCAAAGAGAATGCAAC
GTTTCTGAACAGTACCTCAGAGTCCTGTCCATCCCAGGCAGAACCCTCATTTTGTATTGATTGCCTTCACTTACAGTCTGCACCATGCGAAGTCTGAGCC
AAATCATTTCCCTTTGGAGCTTATTTTCTCAGTGTGCATTCTGCACCAAATTGTACATATCTGTCCCTGCATCTGTCCCTCTCAGCCAGTCATTCATATC
AGGTGCTCTGTGTGTGTTCTGCACAATATGGCAAAGATCTGTAGCATGCGGTTATATACAGCAGCCTAGATTCTTTCTCTATGTAGAGGTTTAGTAGAGC
TAAATTTGGGGTAAATAATTTTGAATATATTTATTTTTATATTGTTTGTAAATGTATTGTAACATTACTTTTATATAAAATAAAACCCACAGGTCTGTGC
TAAGTTAAAAAAAAAAA
5' EST: DN067892 [+]ccagcccctaccccctaccccctagttggacaacgggatggggctgctgagttctgtggtgtttttgctgatctttgtattcgataaaaattgtggtgag
gtgtgcgataacggccggttgaatattcatgtagaccttccagacaagagtgcgtatacgtgtcccaggggacactggccttacccagttcaggtgtttc
caagcataaccacccaatatgaaccagagaaatcacaccgcatctgtatggacactgagatagcgtatgcaaccccaattcccaacagcggtatgcaccg
tccaaaatgggccagatatggagaatatgtgtattgccccccacagcgctgggtgcacaaccttgagcacggaggcgttgttttcttgtaccatccttgt
gttcaccccaaactgaagaaagctttgtcccatggagcccgctcatgcatatacaagcacataatcacccctcacatgaacctttcaactgaacggcctc
ttgctctggtcacttggggctccactctggagatgtcccatatagatctctatgaagttaaaaactggctaatggccaacatgggcaggactagagagca
agagatgaaaatagatgggttgtatcagcacttgcttatccagccagcatcaggggtgtttgatgatcacaacaagaacatttgcccaagggtttgctgc
aagcaagtaaaaagtggtttgaaaagaagaagaagggaccccgcatcacttggttctgccctcatattaacaagtactcctatttatagtgcag
3' EST: DN067891 [+]tttttttttttaacttagcacagacctgtgggttttattttatataaaagtaatgttacaatacatttacaaacaatataaaaataaatatattcaaaat
tatttaccccaaatttagctctactaaacctctacatagagaaagaatctaggctgctgtatataaccgcatgctacagatctttgccatattgtgcaga
acacacacagagcacctgatatgaatgactggctgagagggacagatgcagggacagatatgtacaatttggtgcagaatgcacactgagaaaataagct
ccaaagggaaatgatttggctcagacttcgcatggtgcagactgtaagtgaaggcaatcaatacaaaatgagggttctgcctgggatggacaggactctg
aggtactgttcagaaacgttgcattctctttgccattggcttctgttaaagctgccgtcattcttttataaacagctgtagatccctgggatctctctaa
aagtgaagcatgatcattttttggagatagagagaaataaaatccaatgttttctatagtttcttttgcagacttcattctggatttctccattaagttg
cccttttcgagtgcagagtgcagttcacccaataacgtaaagtgcttttgtgcagaatgggcaccttcccagaaatgaaccctttactctgagctttcac
ttaaactttcatagagcagcactggggttttcttgtgtttaatccacttcttccttttggaatgacctgacatcagcctcctcttgataacagaagccac
ggtctctgtctcagtggcgattccttctgacggccaatacagactttggctcttgcg
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1783 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: lung | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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