XB-CLONE-3434207
Clone Name: IMAGE:7809574
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Gene:
ndst2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168449 [+]
gggcggggaacataccataggcagggggaagcggacaaacgttctaacttcctgggctcatttggactgttcttcccggatccagttttgcctgcagctt
tctgttctctaacaggagatctacggagcagccgcagggcacaacctggaagaacggattaaacaactgctggattaatcttggggagggagcggttctg
gacagctacagcctttcctgaccaagaggaaggaggcatgtaagaagcgactggcatttaatctcgtggaggaaatctattaaagagcccagatcggagt
gaatagaagcaatgggaattggaggccatggtaacctgacataaatggcaggctggagggattctcgcctcttctattcctgctttatttgtttattctg
gttctgcagtttctgtttccctgaggaatttcacattgttatactgaacaggcaaaggacagtgatccagcagtttgggtgagtgttggtaggggggtga
ggtgggtggcctcaccatgattcatctctggaaacttgtgcgtcacatgcgacagttagagctccatcgcttgatcttgctacttattgccttcagctta
gcttccatgtgtttcttggcttactatgttactaacagcccaaaaatcaaggagccaccccctctacccttcagtgactgcagcagccagcagagaactg
tggtgcagccccaagctagctggcgaattgcaaaatctgtggatacttcccgtaccgaaccagtggtgcttgtgtttgtggaaagtatctactctcagct
tgggcaggagatcgtagccatcttagaatccggacgttttaaatatcggacagaaatagcccccggaaagggggacatgccaacgttgactgacaaagat
cggggccgatatgctctaatcatttatgagaatatactgaagtatgtgaatttagatgcttggaacagggaactactagataaatactgcgtggaatatg
gggttggtatcattggtttctttaaagccaatgagaacagtttacttagtgctcaactgaaagggttccctcttttcctacattccaacctgggattacg
ggactaccatatcaacccaaatgcccctcttctttatgtcaccagagcaaacgaagtagagcagggtccactccctggggatgactggacagtgttccag
tcgaatcatagcacatatgaacctgttttgctagctagcaccaaatcggcagaatcgatcccccaccttgccacacacaaggcacttcatgcaacagtgg
tacaggacttgggccttcatgatggaatccagagggtgctttttggcaacaatcttaacttttggctgcataagctaatatttgttgatgctattgccta
cttgactggcaaacgtctctgcctcacccttgaccgttatgttttggtggatatagatgatatctttgttggtaaagaaggcactcggatgaaagtttct
gatgttgaggccttgctgaacactcagagcaagctgagaacacttgttccaaatttcacattcaacttgggtttctctggaaaattctatcacacaggca
cagatgaagaagacgagggagatgacatgttgcttctacacaggaaagagttctggtggtttccccacatgtggagtcacatgcaacctcacttgttcca
taatgtcaccgtccttgcagagcagatgaaactcaacaagcagtttgcccaagaacatggtattcctatagatttagggtatgctgtggcacctcatcac
tcaggtgtgtacccaatccacagccaactctatgaagcctggaaatcggtatggaacatccaggtaaccagtacagaagagtatccacatttaaggccag
cccgcttccggagaggtttcattcacaacaatattatggttttaccacgacagacttgtgggcttttcactcacaccatattttacaacgaataccctgg
tggctccaaagaacttgacaaatccatccgtggtggtgaacttttcctgaccgttctcctcaacccgatcagcatcttcatgacacatctctcaaactac
ggcaatgaccgcctggggctctacacctttgagagcttagtgaagtttgtgcagtgttggaccaacctccggctacaaactctcccacctgtccagctgg
caaagaaatactttgaaatcttccctgaggaaaagaaccctttgtggcagaatccctgtgacgacaagaggcacaaggatatctggtcgaaagagaagac
ctgcgaccgattacccaagtttcttgttgttggaccccaaaagactggtacgactgcactatatacctttctcagcatgcaccctgctgtcactagcaac
tttcagagccccgtcactttcgaagagatccagttttttaatgggataaactaccacaaaggcatcgactggtatatggactttttccctatgccatcca
atgccagcacagactttatgtttgaaaagagcgctaactactttgactcagaaatggttccaaaacgagctgctgctctcctccccagagccaaagtcat
tgccgtcctgatcaatccggcagaccgagcttattcctggtatcagcaccagcgagcacacaatgatccagtagcactgaactacacctttgctcaggtg
gtatatgcaggcctgaatgcacccctggaactgcggaacttgcagaaccgctgcctgatgcctggatggtacgctgtgcatttggacaggtggcttgcct
acttccctgctaaccaggttcacattgtggatggccaggagctgcggagtgacccagccactgctatggacagcatccaaaaattcctaggagtgacgcc
acattttaactacacccaggcccttaagtttgatgaggcaaaaggcttttggtgccagttggcggataatggaaagacaaaatgccttgggaagagtaaa
ggccgaaaatacccagatatggagcagtcgtctcgtactttcctcacagacctctttcgggaaaacaacattgaactttccaagctgttgaaccgattgg
gtcaccctctgccaacatggctacgagaggtgctccagaattccagccggagctgagtattgactccctttggcaaccgcatctagagaatggcggcagc
aatgagaaggaaacaatcagttgctgtttgctcccgttctagaagtgaataacactatgattgctgacattgcttgtggctgtgattaactgtgttctca
ttgctgtggtacgtggacctctcgtgtgccagccacctggaacgcgggaggtgggcggatgcaactcattgtacaaaagtgttacaaaacttgtggcctc
tgacaaaccaagaggaatggaattgcacaaagcagcttcaccctaacaaggaacttagggcattgactactctcccgagtttggggggtgacggtacgaa
tggttctttaggcttcatagtattgtcagtttaacatagccgaggagaagcaacttctttagagcagctcctctggctacagatttaccgtaagctgtgg
aaaagagcatttaagtccatttcacactgggacctttcttacctggtttcttttaaacgcacaatgggaatatattcatgtccccctaccaataatgcat
caggctaaatgtgagatttaaaggaaaataagaataaaccccagcatctgcagctatccccgctgccttctatggttctcaagtgctgcatgtttcctgc
agtcattcatgtatctctgatctttcttgctggagttgctagcagtgtgtatttccaggacctcagtttaataagcgggtgggtgggtgtcagctctccc
atatgctcaagaccacttggcctggatacatatgcacttgtatggagagggaaagtcattgctctctgggtgcctcccattcattggtacttctgggctt
cttgcataaagatcattttattggtcaggagatttattatgaaaaaaaaaaaaaaaaaa
5' EST: DN068008 [+]ggcggggaacataccataggcagggggaagcggacaaacgttctaacttcctgggctcatttggactgttcttcccggatccagttttgcctgcagcttt
ctgttctctaacaggagatctacggagcagccgcagggcacaacctggaagaacggattaaacaactgctggattaatcttggggagggagcggttctgg
acagctacagcctttcctgaccaagaggaaggaggcatgtaagaagcgactggcatttaatctcgtggaggaaatctattaaagagcccagatcggagtg
aatagaagcaatgggaattggaggccatggtaacctgacataaatggcaggctggagggattctcgcctcttctattcctgctttatttgtttattctgg
ttctgcagtttctgtttccctgaggaatttcacattgttatactgaacaggcaaaggacagtgatccagcagtttgggtgagtgttggtaggggggtgag
gtgggtggcctcaccatgattcatctctggaaacttgtgcgtcacatgcgacagttagagctccatcgcttgatcttgctacttattgccttcagcttag
cttccatgtgtttcttggcttactatgttactaacagcccaaaaatcaaggagccaccccctctacccttcagtgactgcagcagccagcagagaactgt
ggtgcagccccaagctagctggcgaattgcanaatctgtggatacttcccgtaccgaaccagtggtgcttgtgtttgtggaaagtatctactctcagctt
gggcaggagatcgtagccatcttagaatccggac
3' EST: DN068007 [+]cataataaatctcctgaccaataaaatgatctttatgcaagaagcccagaagtaccaatgaatgggaggcacccagagagcaatgactttccctctccat
acaagtgcatatgtatccaggccaagtggtcttgagcatatgggagagctgacacccacccacccgcttattaaactgaggtcctggaaatacacactgc
tagcaactccagcaagaaagatcagagatacatgaatgactgcaggaaacatgcagcacttgagaaccatagaaggcagcggggatagctgcagatgctg
gggtttattcttattttcctttaaatctcacatttagcctgatgcattattggtagggggacatgaatatattcccattgtgcgtttaaaagaaaccagg
taagaaaggtcccagtgtgaaatggacttaaatgctcttttccacagcttacggtaaatctgtagccagaggagctgctctaaagaagttgcttctcctc
ggctatgttaaactgacaatactatgaagcctaaagaaccattcgtaccgtcaccccccaaactcgggagagtagtcaatgccctaagttccttgttagg
gtgaagctgctttgtgcaattccattcctcttggtttgtcagaggccacaagttttgtaacacttttgtacaatgagttgcatccgcccacctcccgcgt
tccaggtggctggcacacgagaggtccacgtaccacagcaatgagaacacagttaatcacagccacaagcaatgtcagcaatcatagtgttattcacttc
tagaacgggagcaaacagcaactgattgtttccttctcattgctgc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1783 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: lung | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: