XB-CLONE-3433319
Clone Name: IMAGE:7809706
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Gene:
avl9
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123990 [+]
CTGAGACACTGGGTAGGAAGCAAAAGAGAGTGGGGAAGGGAGTGAGCATCTGCCTTAGGAGAAGGAGGAGATCCTGGCAGGGAAATCAGCAACAAAGGGA
GAGGCTGGACGCGGAAAAGATAAGGGAACCAGATAAGAGTGAATGCTGGGAAGAAAGCGCTAGGGGAGTTGACTTTTAAACTGAGAGGCTATCCCCAGGC
GTTGCATACCCCCGCTGCGGTATTCCTTTGCTGTCTGGACCCGGATTATAGTATGTCCGCGACTTGTTCCAGGGGAGTTCTGTGCTGACAATCCTCACAG
CCTCCGCAGGAACGGCAAGCAAGCGGGGGGCACAGCAGAGCGTTTCCTGACATCTCCGGCACTAACCCCACGCATGGATGCCCAGGCTGCGGAGGAGGCT
GCTGGGGGCGGCAACAGGGTGCCAGGGGGACCTGTACTGCACATAGTGGTGGTGGGCTTCCACCACAAGAAGGGCTGCCAGGTGGAGTTCTCTTATCCTT
CTTTAATGCCAGGGGAGGAACATGATAGTCATATTTTACCCGAGGAATGGAAGTACCTACCATTTCTTGCTTTGCCCGATGGAGCACACAATTTCCAGGA
AGACACAGTGTTTTTTCATCTTCCACCGAGATGTGGAGACCAGAAAACAGTTTATGGTGTTTCATGTTACAGACAGATTGAAGCTAAGGCCTTAAAAGTG
CGTCAAGCTGATGTTACCAGAGAAACAGTACAGAAAAGTGTTTGCGTACTGAGCCAACTGCCTCTCTATGGTCTTCTTCAAGCAAAACTCCAACTGATTA
CTCATGCATATTTTGAAGAAAAAGATTTCTCCCAAATCTCAATTCTTAAGGAACTTTATGAACACATGAATGGCTCGTTAGGAGGAAGTGCAATAGAAGG
ATCACAGCTTTATCTTGGTTTATCTCCAAGAGACCTAGTCCTTCATTTCAGGCACAAGATTCTAATTTTGTTTAAACTAATTCTGCTGGAGAAAAAGGTT
CTGTTTTATGTTTCCCCAGTGAATAGATTGGTGGGTGCACTGATGACTGTCTTATCTCTCTTTCCAGGTATGATTGAGCATGGTCTTACAGACAGTTCCC
AGTACAGGCCACGGAAGAGCATGTCAGAAGACATAGAATATCCTTTAGTTACATATTCAGACGACTGTACTTCTTTGGCTGCCTCAGGGTTAAATGCTAA
ACAAGAAAACCAAGGACCCAATAGGAATACAGAGAATAGCAAGATAGACACATTACTTTGTGAGCATGAGGCTGACACTAATGGCAGTATTGAAAAACTG
AAGCCTCCCTCACATACGTCTCCAGAGTCTGTTGAGAGTGACTGGGAAACTTTGGATCCTAGCATACTGGAGGAATGTATATCAAGGGATGAGGAGGTAA
TTTGGAGTACAGCACCAGAGCACCCAGACTTGCTTCCTACGGATACAATCAGCTCGGGCAGTGCTCCCATAACAGTTCAGCCACAGTCAAACACAGGACA
GTCTGTATACATTCCAGGTCTTATATCTGGATTAGAAGAAGACCAGTATGGAATGCCCTTGGCTATTTTTACTAAGGGCTACCTGTGCTTGCCATACATG
GCACTGCAACAGCACCACCTCCTCTCTGATGTCAGTGTACGTGGCTTTGTAGCTGGAGCTACGAATATTCTCTTCAGACAGCAGAAACACCTAAGTGATG
CCATTGTTGAGGTAGAAGAAGCCCGAATCCAGGTTCATGATCCCGAACTGAGGAAGATCCTTAACCCAACAACAGCCGATCTTCGATTTACTGACCATCT
GGTTAAGCATGTAATGGAAAACAGAGATGACGTGTTTCTTGATGGAACAGGCTGGGAGGGAGGAGATGAATGGATCAGAGCACAGTTTGCACTCTATCTC
CATTCATTGCTTTCTGCCAGTGTACAGCCAGATAATGAAAAAATGGTTTCAGATTATGGGTCTGCCTTCATTGGGGCATGGAAAAGTACGCACAGTTATA
GGGTGTGGAACAGCAACAAGCACCCCGCTCTAGCGGAGGTAAATCCTTGCCACCCATTCCAAGGTCATTACAGCGTAGCTGATGTAAGGTTGAGACTTTC
ACACTCTGTACAGAATAGTGACCGAGGAAAAAAGATAGGCAACGCTGTGGTTACAACAAGCCGAAGTATGGTCCAGACAGGAAAAGCTGTAGGCCAGTCT
GTAGGAGGAGCTCTAATCAGTGCAAAATCTGCTATGTCTTCATGGTTCTCCACATTTACTCAGCCATCAGCCTTGGAACCACAGGATGAGAAGCAGTAAA
TCTGAAAAGATCTTTTTGCTTTGTGACTTTTTATTGTGATTTTTTGTTGTGCCTCATGGTTGCAGCTCACGTTTCCATAGAGACACTTGAGGGTCCATAA
AGACCAGTTTTGTCCTTATCGGATACATTGACACTGGAAGAGATGCTCGGCTTTTGTTTCAAAACGTAAGGCTTAGAAGCACTAGCTGATGAACATATAT
GTTTATTGTTAGTTTACATTCTGCCATATCTTTTACTTGAATCAGTTTAAAAAGAGCTGCAACAAAGGTTAATAAGGTGATGTTTCTCTTTAGATATATA
CAGCAGAAATGTTTTATCCAACATTATCAAACAGATTTACAATAAAAAAATATTGTAAATATTCCACATACTTTATATTCCAAATTATGTATATCTAAGG
TGCAAACAGCAAACACATGGGTTTGAGTTAGCATACCTTGAAATTGAAACTGAAGTTTCTAAATTAGTGGATGACTGGAATTCCTGGCATCAGTTACCAC
AAGGCTGTGCAAGTCATTAAAAAGTTATTCTTCCACCACCAAAAAAAAAAAAAAAAAA
5' EST: DN068608 [+]ctgagacactgggtaggaagcaaaagagagtggggaagggagtgagcatctgccttaggagaaggaggagatcctggcagggaaatcagcaacaaaggga
gaggctggacgcggaaaagataagggaaccagataagagtgaatgctgggaagaaagcgctaggggagttgacttttaaactgagaggctatccccaggc
gttgcatacccccgctgcggtattcctttgctgtctggacccggattatagtatgtccgcgacttgttccaggggagttctgtgctgacaatcctcacag
cctccgcaggaacggcaagcaagcggggggcacagcagagcgtttcctgacatctccggcactaaccccacgcatggatgcccaggctgcggaggaggct
gctgggggcggcaacagggtgccagggggacctgtactgcacatagtggtggtgggcttccaccacaagaagggctgccaggtggagttctcttatcctt
ctttaatgccaggggaggaacatgatagtcatattttacccgaggaatggaagtacctaccatttcttgctttgcccgatggagcacacaatttccagga
agacacagtgttttttcatcttccaccgagatgtggagaccagaaaacagtttatggtgtttcatgttacagacagattgaagctaaggccttaaaagtg
cgtcaagctgatgttaccagagaaacagtacagaaaagtgtttgcgtactgagccaactgcctctctatggtcttcttcaagcaaaactccaactgatta
ctcatgcatattttgaagaaaaagattctcccaaat
3' EST: DN068607 [+]ggtggtggaagaataactttttaatgacttgcacagccttgtggtaactgatgccaggaattccagtcatccactaatttagaaacttcagtttcaattt
caaggtatgctaactcaaacccatgtgtttgctgtttgcaccttagatatacataatttggaatataaagtatgtggaatatttacaatatttttttatt
gtaaatctgtttgataatgttggataaaacatttctgctgtatatatctaaagagaaacatcaccttattaacctttgttgcagctctttttaaactgat
tcaagtaaaagatatggcagaatgtaaactaacaataaacatatatgttcatcagctagtgcttctaagccttacgttttgaaacaaaagccgagcatct
cttccagtgtcaatgtatccgataaggacaaaactggtctttatggaccctcaagtgtctctatggaaacgtgagctgcaaccatgaggcacaacaaaaa
atcacaataaaaagtcacaaagcaaaaagatcttttcagatttactgcttctcatcctgtggttccaaggctgatggctgagtaaatgtggagaaccatg
aagacatagcagattttgcactgattagagctcctcctacagactggcctacagcttttcctgtctggaccatacttcggcttgttgtaaccacagcgtt
gcctatcttttttcctcggtcactattctgtacagagtgtgaaagtctcaaccttacatcagctacgctgtaatgaccttggaatgggtggcaaggattt
acctccgctagagcggggtgcttgttgctgttccacaccctataactgtgcgtacttttcca
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1783 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: lung | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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