XB-CLONE-3432070
Clone Name: IMAGE:7812017
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Gene:
lyset
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127355 [+]
CTAAACCCACATCTATCTCTGTCACTCTGAATCTCGGGCAAGATTTTGTGCTCTAATGTGGAGAGTGGAATTTGTAGCTTGCCTGCGGCCGTCCTGCCAA
TTCTGTACTGCAATGTGCAGCATCCTTAGTGCTGGCAGAGCATGGAGAATGATGAATTTTCGCCAGAGGATGGGATGGATTGGCGTCAGCCTCTACCTCT
TTGTGAGCGCTGCTGCCTTTTACTATGTATTTGAAATAAATGAGACTTATAACAGGCTTGCCTTGGAACATGTACAGCTGAAACCACAGGAGCCACACAG
AGGGACCACATGGACACACTCCTTAAAAGCACGTCTGCTCTCCTTACCTTTTTGGCTGTGGGCTGCTCTTTTCCTGATTCCCTACTTTCAAGTATTTCTA
TTTCTATACTCATGTACAAGAGCTGACCCCAAAACCGTTGGGTATTGCATCATTCCTATATGCCTGGCAATTATCTGCAACCGTCACCAGTCGTTTGTCA
AGGCATCAAATCAGATAAGCAGACTGCAGCTAATTGATACATAAACTGTAAAATCATTTCCTCAAAGGAAGAATAAACCAACCATGACAGGAAATCGAGA
CAATCTGCTTTCTTCTCTACCATTTTGCTGCAATAGAGGGCCTGTCTTGCTGCCTCTTGGGGAGACGTAATATCTAAGTAAAACCAAAGCCAATTTGTTT
TTTGTAACAAATGGGATGTTAAATCTTACATTTTATTTCATTCAGACTTTGTATCAAAGGGCTACAAAGGTGTTCCCTTCATTCATATAATGTTAGTAAA
GAAAATAGCTGGGTTTCGCAAATTTTAGACATGGCATTTATGCATTTGTCCTTGTCACTAAAGTTAGAGAGGATCAGCTAGCGGGAATAGAAGGATTTGG
TAAGAATGAAGGGAAATACAACTGTCACAATATTATATTCTGTAAGTAGGCAAAATGAAATCTGTTCTTAAAACGTTAACAGGAGCATCAGGAGACCCAT
GTTCATTTTAGCAATCAATTGACTGCTATTCTGTGTGAATGGATAGGTACAGGTGCTCCACTTTGCAGTAAATGTGGTACATAATGTGAATCATAGAAAT
GGTCTGGGGTGGGCACTGTCATGTTTCTCTTTAAATAGTCTACTTGGAAATGTGTTAGTGCCATTTGTGGGATTACACAGCATTTCCCTTTAAGTGATTT
TTATTCAGAAATTAAAGTCCATGGGTTTGATTCAGAGGTTTGGCACATGAGAAATTTTCAGTAGTACTATTGCACTTGCCTTGTGTACAGTCCTAGTTCT
GCACTCATTCAGTACAAAATAAAAACACAATGCTACATCTCTTTTGTGTGTTAAAAGAAGTAGGAGCTTACAGGAAATTTTGAAATAATGTATTTTATAT
TGTTTTCCTGCCTTGACATTTTTTACTGAAATTGGATTAATATTTTGAGGAATAATGTTCACATGGTCACATTTTTATGTGTGCGTAGGAAACATTGGGT
TTGGGCTTATTGCATACCTTGTAGATGTAATTTATGGGAAGCAATCAATAAAATAAACAGGAGGTTAACACTGAAAAAAAAAAAAAAAAAA
5' EST: DN072167 [+]ctaaacccacatctatctctgtcactctgaatctcgggcaagattttgtgctctaatgtggagagtggaatttgtagcttgcctgcggccgtcctgccaa
ttctgtactgcaatgtgcagcatccttagtgctggcagagcatggagaatgatgaattttcgccagaggatgggatggattggcgtcagcctctacctct
ttgtgagcgctgctgccttttactatgtatttgaaataaatgagacttataacaggcttgccttggaacatgtacagctgaaaccacaggagccacacag
agggaccacatggacacactccttaaaagcacgtctgctctccttacctttttggctgtgggctgctcttttcctgattccctactttcaagtatttcta
tttctatactcatgtacaagagctgaccccaaaaccgttgggtattgcatcattcctatatgcctggcaattatctgcaaccgtcaccagtcgtttgtca
aggcatcaaatcagataagcagactgcagctaattgatacataaactgtaaaatcatttcctcaaaggaagaataaaccaaccatgacaggaaatcgaga
caatctgctttcttctctaccattttgctgcaatagagggcctgtcttgctgcctcttggggagacgtaatatctaagtaaaaccaaagccaatttgttt
tttgtaacaaatgggatgttaaatcttacattttatttcattcagactttgtatcaaagggctacaaaggtgttcccttcattcatataatgttagtaaa
gaaaatagctgggtttcgcaaatttagacatggcatttatgcatttgtccttgtcact
3' EST: DN072166 [+]cagtgttaacctcctgtttattttattgattgcttcccataaattacatctacaaggtatgcaataagcccaaacccaatgtttcctacgcacacataaa
aatgtgaccatgtgaacattattcctcaaaatattaatccaatttcagtaaaaaatgtcaaggcaggaaaacaatataaaatacattatttcaaaatttc
ctgtaagctcctacttcttttaacacacaaaagagatgtagcattgtgtttttattttgtactgaatgagtgcagaactaggactgtacacaaggcaagt
gcaatagtactactgaaaatttctcatgtgccaaacctctgaatcaaacccatggactttaatttctgaataaaaatcacttaaagggaaatgctgtgta
atcccacaaatggcactaacacatttccaagtagactatttaaagagaaacatgacagtgcccaccccagaccatttctatgattcacattatgtaccac
atttactgcaaagtggagcacctgtacctatccattcacacagaatagcagtcaattgattgctaaaatgaacatgggtctcctgatgctcctgttaacg
ttttaagaacagatttcattttgcctacttacagaatataatattgtgacagttgtatttcccttcattcttaccaaatccttctattcccgctagctga
tcctctctaactttagtgacaaggacaaatgcataaatgccatgtctaaaatttgcgaaacccagctattttctttactaacattatatgaatgaagggg
acacctttgtagccctttgatacaaagtctgaatgaaataaaatgt
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1783 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: lung | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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