XB-CLONE-3430804
|
Clone Name: IMAGE:7819319
|
Gene:
npepps
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: DN085169 
[+]
ggtgctaaaagtggccccctccattcggcacctgctcagtccccagtcaactccttagggaatttattcaattgctcagccttcctaaagaggtagcata
cagctgtgtgtgacaactgtgaatttactaggtggcccataaacaaaggaactctgagcccattttggagcagagttctgtaagttagaggtaccaggca
caagtgcagaggattatttgcaggtaaaggggctgtaacctgacaggttatgacagttatgtgctttacccatggccgcgtaggaggattgctggctttc
cccttttctttaataaggagatactcccctccctctcactccttactgttgccactgtcaaaatctaaccatatgccgactgttgtgtttgtcctggagt
ttaacctaaccctttactctgggcatagagtttaacttgaggggcagttcaagtttgcacgtttgtgtacttacacttaaccccatttccagctgctctg
tacctttcagtggcctggaccgttatcccccaacagtacagaaaatgcttgcacagtggtactgaagggtctccttaatgtagatttctcctcttagcca
cactgaagagggacaaagtgcaaagtatcttgttcctaaagtcaactagttataccagttccagataaaaccctaggttagccagtttgcaggtattgtg
cagtctaacggactagcggttgggcaataagccttggttatcagacttgcttacctgagttctctcttttgtgctattgttttaaatgacaaanacaaaa
aag
3' EST: DN085168 [+]aaacacgaacattttgtttttattaacgccaactttttttttttctttttaattcttttttgtttttgtcatttaaaacaatagcacaaaagagagaact
caggtaagcaagtctgataaccaaggcttattgcccaaccgctagtccgttagactgcacaatacctgcaaactggctaacctagggttttatctggaac
tggtataactagttgactttaggaacaagatactttgcactttgtccctcttcagtgtggctaagaggagaaatctacattaaggagacccttcagtacc
actgtgcaagcattttctgtactgttgggggataacggtccaggccactgaaaggtacagagcagctggaaatggggttaagtgtaagtacacaaacgtg
caaacttgaactgcccctcaagttaaactctatgcccagagtaaagggttaggttaaactccaggacaaacacaacagtcggcatatggttagattttga
cagtggcaacagtaaggagtgagagggaggggagtatctccttattaaagaaaaggggaaagccagcaatcctcctacgcggccatgggtaaagcacata
actgtcataacctgtcaggttacagcccctttacctgcaaataatcctctgcacttgtgcctggtacctctaacttacagaactctgctccaaaatgggc
tcagagttcctttgtttatgggccacctagtaaattcacagttgtcacacacagctgtatgctacctctttaggaaggctgagcaattgaataaattccc
taaggagttgactggggactgagcaggtgc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: ovary | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
