XB-CLONE-3428747
Clone Name: IMAGE:7820421
|
Gene:
oat.2
,
oat
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC167123 [+]
cgtatccagaatgctttccaaactgatccagagacgcaccctgggtgttttctatggcaatgtgaaagtgtctatgagctctgccacaactgctgccaca
gagaaaccagcggatgaagctttatcatcggaatgtgtgtttgaacgggaatccaaatacggagcgcacaactaccaccctctgcctgtagcacttgaaa
gaggaaaaggcgtgtatgtttgggatgtcgaaggcaggcggtattttgatttcctcagtgcctacagtgctgtgaatcaggggcactgccatccaaagat
cctcaatgcactgaaggcccaggcggacaaactcactctcacttccagagctttctacaatgacgtgctgggcgaatatgaggaatatgttacaaagctc
ttcggctacaacaaagtgctgcccatgaacaccggtgttgaaggtggagaaacagcatgcaagctggccagaaagtgggcctacaccgtgaaagggatcc
ccaagtacaaggcccagattatctttgcagccggaaacttttgggggaggacaatgtctgcaatatccagctctacagacccatctagctacgagggttt
tggaccattcatgccagggtttaaaattattccttacaatgaccttcctgctcttgagcgagcgttacaagaccccaatgttgctgctttcatggttgag
ccgattcagggggaggccggcgtgattgttccagacgatggctacctgacaggggtgcggcaactgtgcactgcacacaatgtgctattcattgctgatg
aagtacaaacgggcctggctagaacaggcaagatgctagcagtggaccatgaaaatgtccgcccagatattgtgatacttgggaaggccctgtctggagg
agtttacccagtgtctgctgttctgtgtgatgatgaagtcatgctgaccataaaaccgggagaacacggatctacctacggtggaaatcctctggcgtgt
cgcgtggcaatggcttctctagaggtcattgaagaggagaagctggcagaaaatgcaaccgcaatgggagagctgctgagagcagagctgatgaagactc
catctgacatagtgacggctgtaagagggaaaggcctactgaacgccattgtcataaaggaaagcaaagactgtgacgcatggaaggtgtgcctccggct
acgtgacaacgggcttttggccaaacccacccacggcgatataatccgactggctcctcctctaaccatcaaggaagacgagattagagaatgcactgaa
atcatccacaaaaccctgctgtctttctaagcagccagtcactcccttggtgtcatgtcgtcttatgttgcacgttccgtcactcatgtctctgattgga
tggtgtgttgttactgagactattctggggcttcctggaattggttgtactggaactaaaggtcactggaagatcagcctgagctgtgcttccctgatgc
ttcggtgcaaactcctgtacagtagcgcagtcaccgctgcagatgcaacagtgtcactggctttgtctgtgcccaaaagccctgcaaagtcactggccct
gatgcatttagcaagcatgattgtctgtatgtagtgcagagggggcagaatagttcagtctgagtcatgggtcgccccctgccctgaattagtgtatagt
tttatatgtggtgcaggtcttagtgcatttaaattaacaggactttatttaatatttagcttaatataagataaactacatttattgctttgtaatgatg
aaaagaaataaagatgaattctctaattccaaaaaaaaaaaaaaaaaa
5' EST: DN086622 [+]cgtatccagaatgctttcccactgatccagagacgcaccctgggtgttttctatggcaatgtgaaagtgtctatgagctctgccacaactgctgccacag
agaaaccagcggatgaagctttatcatcggaatgtgtgtttgaacgggaatccaaatacggagcgcacaactaccaccctctgcctgtagcacttgaaag
aggaaaaggcgtgtatgtttgggatgtcgaaggcaggcggtattttgatttcctcagtgcctacagtgctgtgaatcaggggcactgccatccaaagatc
ctcaatgcactgaaggcccaggcggacaaactcactctcacttccagagctttctacaatgacgtgctgggcgaatatgaggaatatgttacaaagctct
tcggctacaacaaagtgctgcccatgaacaccggtgttgaaggtggagaaacagcatgcaagctggccagaaagtgggcctacaccgtgaaagggatccc
caagtacaaggcccagattatctttgcagccggaaacttttgggggaggacaatgtctgcaatatccagctctacagacccatctagctacgagggtttt
ggaccattcatgccagggtttaaaattattccttacaatgaccttcctgctcttgagcgagcgttacaagaccccaatgttgctgctttcatggttgagc
cgattcaaggggaggccggcgtgattgttccagacgatggctacctgacaggggtgcggcaactgtgcactgcacacaatgtgctattcattgctgat
3' EST: DN086621 [+]ggaattagagaattcatctttatttcttttcatcattacaaagcaataaatgtagtttatcttatattaagctaaatattaaataaagtcctgttaattt
aaatgcactaagacctgcaccacatataaaactatacactaattcagggcagggggcgacccatgactcagactgaactattctgccccctctgcactac
atacagacaatcatgcttgctaaatgcatcagggccagtgactttgcagggcttttgggcacagacaaagccagtgacactgttgcatctgcagcggtga
ctgcgctactgtacaggagtttgcaccgaagcatcagggaagcacagctcaggctgatcttccagtgacctttagttccagtacaaccaattccaggaag
ccccagaatagtctcagtaacaacacaccatccaatcagagacatgagtgacggaacgtgcaacataagacgacatgacaccaagggagtgactggctgc
ttagaaagacagcagggttttgtggatgatttcagtgcattctctaatctcgtcttccttgatggttagaggaggagccagtcggattatatcgccgtgg
gtgggtttggccaaaagcccgttgtcacgtagccggaggcacaccttccatgcgtcacagtctttgctttcctttatgacaatggcgttcagtaggcctt
tccctcttacagccgtcactatgtcagatggagtcttcatcagctctgctctcagcagctctcccattgcggttgcattttctgccagcttctccctctt
catgacctct
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: ovary | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: