XB-CLONE-3423030
Sequence:
Full length sequence: BC121997 [+]
CTTGAACCCCCACTATGGAAGGCCTGAAGGAACAGAGGATGCTGGATTTTGTTGTGGAAAATGCAGTGCGTGGTGATCCTCAGAGTGTGGTAGATACTAT
AGACAATTTCTGCAGGAACAAGGAGTGGGCCATGAATGTGGGAGACGAGAAAGGTCTGATCCTAGATGAAGTGGTTAAAGAAACCGATCCTTTGATGGTA
GTGGAGCTGGGCACGTACTGTGGATACTCTGCTGTTCGAATAGCTCGACTGCTGAAGCCGGGAGCTCGCATCCTCACCATGGAGATGAATCCAGTTCATG
CCTCTGTAGCCAGGCAGATGATTGAATTTGCTGGAGTCAACAACACAGTTCAAGTACTGGAAGGGTCCACTGCAGATTTCATCCCGCAGCTGAAGCAAAA
CTATGGCGTGGATACGCTTGACTTTGTGTTTCTGGATCATTGGAAGGACCGATATCTAATTGATACCAAACTTTTGGAGGAGTGTCAGTTGTTGAGGAAA
GGCTCTGTGTTATTGGCAGATAATGTTGTCTATCCTGGCATCCCAGACTTCCTGGAGCACGTCAGGACCTGCGGCCGGTTTCCTTTAAGCACCACCATAC
TGAGAGCTGTTTGACATGGCCCAGAGTAAGGAACAGAGAATTTTGGATTTTGTGCAGCAAAATGCAGTTCATGGGGACCCCCAAAGTGTTATAGACCACA
TTGATGAATACTGCAGTAAAAAGGAGTGGGCAATGAATGTGGGTGATGAGAAAGGTCTGATTCTGGATCAAGTACTAAAGGAGACTGAGCCATTAGAGGT
GCTGGAGCTGGGCACTTACTGTGGATACTCTGCTATCCGGATAGCTCGGCTGCTGAAGCCCGGTGCTCGACTACTTACTGTGGAAATAAACCCAGACAAT
GCTTCTGTTGCCAAGCAGATGATTCAGTTTGCCGGAGTAGAAGACAAGGTGCAGATACTGCTTGGCTCCACCGAGACTGTTATTCCTAACCTGCGGACAC
AGCATGGAGTAAAGCACTTTGACTTTGTATTTATTGACCATTTTAAAAACCGATACAGTCCTGACACTATGTTACTAGAGGAATATGGTTTGCTCAGGAA
GGGCACAGTCTTACTCGCTGACAATGTTGTGCTACCCGGAGCCCCAGAATTCCTTGAACATGTGCGGACAAGTGGTCGCTATGACTGTACCAATTACCCT
GCCCATGTTGAATACAGTGATAAAGTAGATGCTTTGGAGAAGGCTGTGTTTAGAGGGTAGGGGTGCATCATACGGCCCCATCATATTTTAAAACCCAGGA
AATACCAGAGTGACATTAACAGAGGCATATTTACCCAAAGGGGGACTCTTCTGTGTGTGTTCTGGGACTGCACTGCCACACTACCTTATTGAAAGAAAAA
CTTGCTGGATCATTTATCTCCCTGTTCATGTCTATAAATTGTATTTATTCCTTCAGTGGAACATTGCATTCCAGAGGGGTTTGCGTTCAGCTGATATAGA
AACCAAAGAAAGAACTGAAGGCAAGCAATGCATTGTATTGGAATGTGCTATTTTACTCACTTTCTATACATATTTAGTAAATAGACAGTACTGAAAATAA
ACACCATCTTCTTATTCCAAAAAAAAAAAAAAAAAA
5' EST: DN090603 [+]cttgaacccccactatggaaggcctgaaggaacagaggatgctggattttgttgtggaaaatgcagtgcgtggtgatcctcagagtgtggtagatactat
agacaatttctgcaggaacaaggagtgggccatgaatgtgggagacgagaaaggtctgatcctagatgaagtggttaaagaaaccgatcctttgatggta
gtggagctgggcacgtactgtggatactctgctgttcgaatagctcgactgctgaagccgggagctcgcatcctcaccatggagatgaatccagttcatg
cctctgtagccaggcagatgattgaatttgctggagtcaacaacacagttcaagtactggaagggtccactgcagatttcatcccgcagctgaagcaaaa
ctatggcgtggatacgcttgactttgtgtttctggatcattggaaggaccgatatctaattgataccaaacttttggaggagtgtcagttgttgaggaaa
ggctctgtgttattggcagataatgttgtctatcctggcatcccagacttcctggagcacgtcaggacctgcggccggtttcctttaagcaccaccatac
tgagagctgtttgacatggcccagagtaaggaacagagaattttggattttgtgcagcaaaatgcagttcatggggacccccaaagtgttatagaccaca
ttgatgaatactgcagtaaaaaggagtgggcaatgaatgtgggtgatgagaaaggtctgattctggatcaagtactaaaggagactgagccattagaggt
gctggagctgggcacttactgtggatactctgctatccggatagctcggctgctga
3' EST: DN090602 [+]ggaataagaagatggtgtttattttcagtactgtctatttactaaatatgtatagaaagtgagtaaaatagcacattccaatacaatgcattgcttgcct
tcagttctttctttggtttctatatcagctgaacgcaaacccctctggaatgcaatgttccactgaaggaataaatacaatttatagacatgaacaggga
gataaatgatccagcaagtttttctttcaataaggtagtgtggcagtgcagtcccagaacacacacagaagagtccccctttgggtaaatatgcctctgt
taatgtcactctggtatttcctgggttttaaaatatgatggggccgtatgatgcacccctaccctctaaacacagccttctccaaagcatctactttatc
actgtattcaacatgggcagggtaattggtacagtcatagcgaccacttgtccgcacatgttcaaggaattctggggctccgggtagcacaacattgtca
gcgagtaagactgtgcccttcctgagcaaaccatattcctctagtaacatagtgtcaggactgtatcggtttttaaaatggtcaataaatacaaagtcaa
agtgctttactccatgctgtgtccgcaggttaggaataacagtctcggtggagccaagcagtatctgcaccttgtcttctactccggcaaactgaatcat
ctgcttggcaacagaagcattgtctgggtttatttccacagtaagtagtcgagcaccgggcttcagcagccgagctatccggatagcagagtatccacag
taagtgcccagctccagcacctctaatggctcagtctcctttagtacttgatccagaatcagaccttttctcatcaccacattcattgcccactcctttt
tactgcagtattcatca
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: ovary | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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