XB-CLONE-3418299
Clone Name: IMAGE:7824065
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Gene:
gtf2a1l
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157263 [+]
AACATCGCGATAACTCGGCGCTACAGCGGCGCGCAAAGCTCATGTCAGCTCGTTTGTAACGCTCGGTTATACACAACGCCGGGTGGAGCTGCATGGAGGA
CAGACAGGCCGGAGAGTTAGCATGGCCCATTCTGCCAACCCCAATCCTGTGCCAAAGCTATACAAATCAATTATTGAAGATGTTATGGAAAGTGTCAGGG
AACTTTTTGTGGAAGAAGGTGTGGATGAGCAAGTGCTTAAGGATTTAAAGCAATTATGGGAGACCAAGGTACTGCAGTCTAAAGCTACAGAAGGTTTCTT
CAGAGACAACAGTGCCCCACAATTTGTCCTACAACTGCCACAGAATTTACATCACTCCCTTCACAGCTCAACAGGGAATAGGAATGTTACACATTTTTCA
ACTGGCGAAATGGGTACCTCTGGATCGAACAGTGCGTTTTCTCTTCCAGCGGGTATTACTTATCCAATTCATCTGCCAGCTGGAATGACTGTGCAAACTG
CATCTGGTCAACTTTACAAAGTAACAGTGCCGGTGATGGTCACTCAAGCTCCAGGAGGGCCGCGAATACTTCAGCCACCTGTTCAACAGTTCTTCCAACA
TATTCGCCATCCTTCAGGGCATGCTGCAAGCAGCCAATACAGCAGAAATGCTTACATAGAACAAAAATGGCAGCAGCAACCGGCTCAGGTTCACAGTTCT
GAAGGACTAAATATATATGATCAAGAGGCCACCATTGAACCTAATTATCAGAATAATAATAACGATGATTTGATTATTCAGCAGTCCATAGATTTGCAGC
AGAATCTTACAAATAAGGGGCTTCATCAGCCCACAAACAGTTTGGAAGGCAGCAATTTAAATGAAATAACAGGGACGCTCTTTAGTCCTAGGCAGTCCAA
TGGAGGAATAGATCTGAACTCTGAGTCTGTGATGAATAACTTAAACATGGCTCATTCCTTGCAAGGACAACCGGAAAATGAATATGCAAGCCTGTTAAAA
TCTCAAGATTCCGATGATGTCGTTGAGCTAATACTAATGGATAATGAACTTGGTGATAAAACTTCCACAGGAGACAGTGGGAACGTTGGCATCCAGACTG
GGATATCTAATGTGGAGAAGCAAGCAGTACCCCGTGAAATAGAGATCATTCAAGTTGATGGGACCGGTGACACTTCCTCTGATGAAGACCTTGGAAATGT
GAGAGACCTAGATGAAAATGATTTTTTGGGCATTATTGACACAGAGGATCTTAAAGCTCTAGAAGAAGATGATACTGCATCAAATGACTCAACTAGTAAT
AGTAGTGATGATGAGGATCCGGAAATTGATGTCATTGAAGAGGATCCATTAAATTCTGGAGATGATGTGAGTGAGCAAGAAGTCCCAGACTTGTTTGATA
CTGACAATGTCATTGTGTGTCAGTATGATAAGATTCATCGAAGTAAAAACAAGTGGAAATTCTATTTAAAAGATGGAGTCATGAGTTTTGGTGGGAAAGA
TTATGTTTTTTCTAAAGCCATTGGGGAAGCTGAATGGTGATAACCAGAACGCTTTTCCACGGTGTTTAACTTGCAAAGAAGAGAAGGAAGAAAAAAATTC
ATTTGTATTTTTCACCCAAGCATTCTGCACTTAATAATAATAATAACAGCAGAAACAATATCAATTTTGTTGTTTTCTTTAAGTTAAATTTGCATCCTTA
TGAGTGAAATCTACATATTTTTTGTGTTGCAGCAGATATGAAATAAATATAATTATGCAGTAAAAAAAAAAAAAAAAAA
5' EST: DN093862 [+]aacatcgcgataactcggcgctacagcggcgcgcaaagctcatgtcagctcgctttgtaacgctcgcgttatacacaacgccgggtggagctgcatggag
gacagacaggccggagagttagcatggcccattctgccaaccccaatcctgtgccaaagctatacaaatcaattattgaagatgttatggaaagtgtcag
ggaactttttgtggaagaaggtgtggatgagcaagtgcttaaggatttaaagcaattatgggagaccaaggtactgcagtctaaagctacagaaggtttc
ttcagagacaacagtgccccacaatttgtcctacaactgccacagaatttacatcactcccttcacagctcaacagggaataggaatgttacacattttt
caactggcgaaatgggtacctctggatcgaacagtgcgttttctcttccagcgggtattacttatccaattcatctgccagctggaatgactgtgcaaac
tgcatctggtcaactttacaaagtaacagtgccggtgatggtcactcaagctccaggagggccgcgaatacttcagccacctgttcaacagttcttccaa
catattcgccatccttcagggcatgctgcaagcagccaatacagcagaaatgcttacatagaacaaaaatggcagcagcaaccggctcaggttcacagtt
ctgaaggactaaatatatatgatcaagaggccaccattgaacctaattatcagaataataataacgatgatttgattattcagcagtccatagatttgca
gcagaatcttac
3' EST: DN093861 [+]actgcataattatatttatttcatatctgctgcaacacaaaaaatatgtagatttcactcataaggatgcaaatttaacttaaagaaaacaacaaaattg
atattgtttctgctgttattattattattaagtgcagaatgcttgggtgaaaaatacaaatgaatttttttcttccttctcttctttgcaagttaaacac
cgtggaaaagcgttctggttatcaccattcagcttccccaatggctttagaaaaaacataatctttcccaccaaaactcatgactccatcttttaaatag
aatttccacttgtttttacttcgatgaatcttatcatactgacacacaatgacattgtcagtatcaaacaagtctgggacttcttgctcactcacatcat
ctccagaatttaatggatcctcttcaatgacatcaatttccggatcctcatcatcactactattactagttgagtcatttgatgcagtatcatcttcttc
tagagctttaagatcctctgtgtcaataatgcccaaaaaatcattttcatctaggtctctcacatttccaaggtcttcatcagaggaagtgtcaccggtc
ccatcaacttgaatgatctctatttcacggggtactgcttgcttctccacattagatatcccagtctggatgccaacgttcccactgtctcctgtggaag
ttttatcaccaagttcattatccattagtattagctcaacgacatcatcggaatcttgagattttaacaggcttgcatattcattttccggttgtccttg
c
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: ovary | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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