XB-CLONE-3408966
Clone Name: IMAGE:7801911
|
Gene:
ppp4c
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DR834972 [+]
ccggatccgtgaagcttcattgaggagggaccatgactgaaatcagtgacctcgaccggcagatcgagcagctgaggcgctgtgagctcatcaaggagag
cgaagtgaaagctctttgtgccaaagcgcgagagattctggtagaagagagtaacgtgcaacgagtcgattcgcctgttacggtctgtggggatatccat
gggcagttctacgatctcaaggagttgttcagggtgggcggcgacgtccccgagacaaattaccttttcatgggtgactttgtggatcgtgggttttaca
gcgttgaaacattcctccttctcttagcactaaaggttcgttacccagatcggatcaccctgatacgcggcaaccacgagagccggcaaatcacacaagt
gtacggtttctacgacgagtgtctgcgcaaatacggctcggtaacggtgtggcgctactgcacagagatctttgactacctcagtctgtctgctatcata
gatggcaagatcttttgtgtacatggtgggctgtcaccctccattcagacattggatcagattagaactattgaccgcaagcaagaagtgcctcatgatg
gacccatgtgtgacctgctctggagtgacccagaagatactaccggatggggggtgagccccaggggagctggatacctttttggcagtgacgtagtggc
acaattcaacgcagccaacaacattgatatgatttgccgggctcaccagcttgtcatgnaaggatancagtggcacttcaacgagactgtcttaactgtg
tggtcggcaccaaactactgctacaggtgcgggaatgt
3' EST: DR834971 [+]ttttatacaaatacattttccatttttattgctttttctcattttattagcagatgtttaactgtatcctaccaaaaatgtaaaattagaagaaaaaaaa
aataaaaataaaagggaaaaaggataaaatctttgaccagaaagaatgcggaacaagagactggggccatagacgaaaggaagggagttataatggaata
tcggctgttccagggaaggaatcggaggtttggcattgtaggtggcaatacacgagctgaagctttagatgcctccttgccaaagcaatctctcagctag
ccgcttacaaagggttaaacaaaatggctactcacgttcccggcagcccagaatggcttcgctgagctacaggggccagctacggtgaacactttggctc
aaaagttgccaatcggcaccaaaaggggctcaaatcttagatctgccgcagcttctaaatagagaaggggcggcgggttccatcatcatcatcatcatgt
ctctcctggtccccccccagctgggattgggcgcccgggccccgcttgcactaaggcactccgtacgtgagtgtaagctcagcatcttagtacaggggac
gtgaggctccgagtggaagagacattataaggaatgactgcggatctgggggggggggtggttataaaatcaaggaagaagctgtgaattctagaagtat
tttggtttccttgcataatgctgcccccccctctctccctactagaaagcatcacactgaccccccctg
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1905 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: