XB-CLONE-3408509
Clone Name: IMAGE:7801860
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Gene:
actr6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121585 [+]
GGACAACGGGGCTTATAATGCAAAGATTGGTTACAGCCACGGACAAGTGAGTGTAATTCCCAACTGTCAGTTCAGAACCAAAACAGCACGTTTAAAAACA
TTTACAGCTAATCAGATTGATGAAATAAAGGATCCATCAGGCTTATTTTATATTCTCCCATATCAAAAGGGTTATTTGGTCAACTGGGATGTCCAGAGAC
AAGTTTGGGACTACCTTTTTGGGAAGGAAATGTTTCAGGTGGACTTTCCTGATTCTAATATAATTATTACAGAACCTTATTTTAACTTCAGTTCTATTCA
AGAATCCATGAATGAAATTTTGTTTGAAGAATATCAGTTCCAGGCAGCTCTCAGAATTAATGCAGGAGCTTTGAGTGCGCACAGGTATTTTCGAGATAAC
CCATCGGAATTGTGCTGCATCATTGTAGACAGCGGTTATTCATTTACACACATTGTGCCATTCTGCAGAAGTAAGAAGAAAAAAGAAGGCATAATAAGGA
TTAACGTTGGAGGAAAATTAATGACAAATCATCTTAAAGAAATCATTTCATACAGGCAGCTGCAGGTTATGGATGAAACACATGTAATTAACCAAGTCAA
AGAAGATGTTTGTTATGTGTCTACAGATTTTTATAAAGATATGGAAACTGCAAAACTAAAAGGAGAGGAGAACTCGGTGATGGTGGACTATGTTCTGCCT
GACTTCAGCACCATCAAAAAAGGGTTTTGCAAGGTGTGTATGTGTTAGTCTCTGTGGCTCTCTGTATAAGCAAAATGCACTACTTACTGACCCACAAATC
CAATCCTTTACTGCCATGAGCAAAGGTTCACCAGCACAGCCTGAATATATTTTTCTTTAATATAGAGACAACTGCTTTTTAAAGTGCAAGAAAGCTTTGA
TACCGGCGATCAGAGATCAGAGCAGTATACTATAAGTGAGGACATTTGGGCATGAAGTAGTATTCATTTTACATGTTCCTTAGTTAAGATACCTTTAAGG
GCTGTGGCACACGGGGGAGATTAGTTGCGACAAATCTCCCAGGGATGCCATCCCACCGGTGAGGGTGTGGATCGCCGGTGGGATGGCATATGCGGTGCCT
TGAGAGGAAACTTCCAGGATTTCGGGGAAATTGCGGCGCCGCGTATGCCATCCCACCGGGGATTTGCGTTCTGGCCGGTGGGATGGCATTTCAGGGAGAT
TAGTCGCTATCACCTAAACCAGTACTATCTTCCATCTATTGCCTCTTCTGTAGCAGCTACTCTATATGGCCAATAAAATGCTGTATGCATTATAACCCTT
TTGATTAACACACAACCCTTTGTTGTGACAGCTCCTAATACATTTTTTTTTTGAGTAGCCAAGGGAAGAGATGGTGTTTAGTGGAAAGACCACAGCTGGA
GAGCAAATACTACGTTTGACCAATGAGAGATTTGCTGTCCCTGAAATCCTTTTCCATCCCTCAGACATTGGGATTCAAGAAATGGGAATTCCAGAAGCAA
TTGTGCATTCCATTAATAATCTTCCTGAAGAGATGCAGCCTCATTTCTACAAGAACATTGTTCTCACTGGCGGAAACACACTATTCCCTGGTTTTAGAGA
ACGGGTATTTTCTGAGGTCCGAAAGCTCACACCCACAGACTTTGACGTCTCAGTGATATTACCTGAAAACCCCATCTCATATGCCTGGGAAGGAGGAAAG
ATAATATCTGAAAATGACGACTTTGAAGACATGGTGGTAACCAGGGAAGACTATGAGGAAAATGGCCATATTATTTGTGAAGAAAAGTTTGATATTTAGT
TCAGACATTACATAAAAAATGTAAATAATAGGATAAATTTTACTTAATGTATTATTAATTATGAGGAAATGTCATTTTTTTACTATTTATGAAGTAAACT
TGCATTTTCATGAAGTATCTGCATTTGTAAATAAATCTCTTTATAAGAAAAAAAAAAAAAAAAAA
5' EST: DR835273 [+]gacaacggggcttataatgcaaagattggttacagccacggacaagtgagtgtaattcccaactgtcagttcagaaccaaaacagcacgtttaaaaacat
ttacagctaatcagattgatgaaataaaggatccatcaggcttattttatattctcccatatcaaaagggttatttggtcaactgggatgtccagagaca
agtttgggactacctttttgggaaggaaatgtttcaggtggactttcctgattctaatataattattacagaaccttattttaacttcagttctattcaa
gaatccatgaatgaaattttgtttgaagaatatcagttccaggcagctctcagaattaatgcaggagctttgagtgcgcacaggtattttcgagataacc
catcggaattgtgctgcatcattgtagacagcggttattcatttacacacattgtgccattctgcagaagtaagaagaaaaaagaaggcataataaggat
taacgttggaggaaaattaatgacaaatcatcttaaagaaatcatttcatacaggcagctgcaggttatggatgaaacacatgtaattaaccaagtcaaa
gaagatgtttgttatgtgtctacagatttttataaagatatggaaactgcaaaactaaaaggagaggagaactcggtgatggtggactatgttctgcctg
acttcagcaccatcaaaaaagggttttgcaaggtgtgtatgtgttagtctctgtggctctctgtataagcaaaatgcactacttactgacccacaaatcc
aatcctttactgccatgagcaaaggttcaccagcacagcctgaatatatttttctttaatatagagacaactgctt
3' EST: DR835272 [+]cttataaagagatttatttacaaatgcagatacttcatgaaaatgcaagtttacttcataaatagtaaaaaaatgacatttcctcataattaataataca
ttaagtaaaatttatcctattatttacattttttatgtaatgtctgaactaaatatcaaacttttcttcacaaataatatggccattttcctcatagtct
tccctggttaccaccatgtcttcaaagtcgtcattttcagatattatctttcctccttcccaggcatatgagatggggttttcaggtaatatcactgaga
cgtcaaagtctgtgggtgtgagctttcggacctcagaaaatacccgttctctaaaaccagggaatagtgtgtttccgccagtgagaacaatgttcttgta
gaaatgaggctgcatctcttcaggaagattattaatggaatgcacaattgcttctggaattcccatttcttgaatcccaatgtctgagggatggaaaagg
atttcagggacagcaaatctctcattggtcaaacgtagtatttgctctccagctgtggtctttccactaaacaccatctcttcccttggctactcaaaaa
aaaaatgtattaggagctgtcacaacaaagggttgtgtgttaatcaaaagggttataatgcatacagcattttattggccatatagagtagctgctacag
aagaggcaatagatggaagatagtactggtttaggtgatagcgactaatctccctgaaatgccatcccaccggccagaacgcaaatccccggtgggatgg
catacgcggcg
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1905 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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