XB-CLONE-3403522
Clone Name: IMAGE:7640561
|
Gene:
apbb2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX793526 [+]
aaggcaggctatcagccagtactggatggagcactgacagttggacgcacacacattcaagccgttcattagccgagatgagctaaatctccatttgaat
acagacagcaaaatagctgcacaaaggtccagggtttgatctgtttggagaagcactttgtctgctcccttgagccagttgtcatgttctgaccatccac
aatgggcaggcgatatcctggtgcaacctaccgaccaagcagttcatatttctaaccctaaccaatatcctgatccattgctgacgcccttctgatccat
gtctgaagtcctgcaagtcgattcaagtgtggaaacacttccagtttttatggcaagccacagcgccacggacattgtgaatcgaaatgcagttagccca
ccaaataccttgaacttgcgctcttcccacaatgaactattgaatacagatataaagcacgtggaaacgaagagcataactccaccaaaatgcaggaaaa
aatatgcactgacaaatatccaggcagctatgggcttgtctgaccctgcatctcagcctctcctgggaaacagttctccaaacataaagtttgtaaagaa
tggggaaaatcagctgaggaaagctgctgaaaatgggaatgggaatcaagaccaaaataaaaatatgagtttagaagatactgtcaacagaatctcagag
aaactagaagggaaagaagagatcccagatgactcagaaagttctgaaattctaccattaattcctagaagaaccaagagttttctcaattactatgcag
acttggatctctccattgacctggagcaaagcaatgggcatgtagtagaggagggagactc
3' EST: CX793525 [+]tttttttttttttttagtgaacatggctgtttatagagccgctgcatacaaataatgcatagataaatgaactcttactaacacgttggcatttagtcac
acataccacatacatactttttggccgtaaaaaaaagtgacttttttaattacaattggttttacaaaataatcttttaagttaaaatatgcaaagctat
ttttaaaacaaaatatctgcaaaggatgttccttttcccatagatcacttccgccatttcgtctgatgccagagttatggaaatgagagcccctgggcaa
tgaaggaagggccccaatatgagccagatcctgtgccctactattggcacctaatgctggcaccatgttgcacataggaagggcccgtaatgtctgactc
ttacatgaaacacaaagtgctctaggaatatgttgctgccacacttttagggttcctgtttcattctggacccatcctgctacactgatagtgataccca
tgatgcaccagagcagggagagtatcaccgatcactgggggctacactctgcaaattaaactgctgtgtccaactggtgtttgggtaattacaagtcatt
aagggatcaataggccctatttttcccgcagcgctttccaaaacaaacacagatgactatggcggaatgtgagccctaatagctgtaaatggccatgcaa
catggagacatgatttttcagggtgccagtaagtggccacgcggttggatgagttctagcctggcttctgggtatcggccccatactgcctgtagttagc
agctctaggttaataact
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: