XB-CLONE-3402948
|
Clone Name: IMAGE:7640836
|
Gene:
ncam1
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX794451 
[+]
ccatattctagcactgttatggttatttttgatgagccagatgccactggtggtgttcctattttgaaatacaaagcagaatggaaagttataggacagg
agaagtggcatgccaaatattatgatgccaaagaggtgagtgcagagagcatcattacagtcacgggtcttaagccagaaacttcatacttggtgaagct
gtcggcagttaatggcaaaggactgggagacagcacaccttcacaggaattcactactcagccagtcagggaacctagtgctccaaaattggtatggcat
ttgaatgaagatggaaacaatgtaaaagtggagataattaagcaggatgatggtggctctcccatcagacactacctggtcaattacagagcgttaaatg
ctgcagattggaaaccagaaatgcgggttccttctagtagtcaccatgtgatgctcaaggctctggaatggaatgtagagtatgaagttatagtggttgc
cgaaaatcagcagggaaaatcaaagccagctcgtttatcctttagaaccacagccaagcccacagccactacagccaccacaagtgccagtgctggtttg
ggaacaggagctattgtcggcattcttattgtcatctttgttcttcttctggtggttgtggatgtgacctgctttttcttgaacaagtgtggcctactta
tgtgcattgctgttaacttctgtgggaaagctggacctggcgccaaaggcaaagacattgaggaggggaaggcagctttttcaaaagacgagtctaaaga
gcccattgtagaggtgcgaacagaagaag
3' EST: CX794450 [+]aaatatacagtgcctaaatcttagaacaatacatactgtatgtacttatacatattgtactgtaggaacttattatcagatgcaaggaggagtcttggtc
cctaatagtaatgtgtcaggaatccctgttaagtgttaaccacatgtaacatagagagagtttcttgttttctgacataatagccaatgactaaacaaaa
ggaggcttaaagcctactgagaataactgtgtttccttctgtgctattccttgcaactccatgtgattgtgttcatcgttagtgttatatggggcgcatt
acagtaataagatattgagatgttctagaccaatgacctgataacattttaactgcttaacaggcaacacatagatcctccattttcatccctctagtac
cgttcaagaaagcatacatgagataacataaaacaatacatggaacattatgaatgtacatttttcacagtttgaatttactgagcaatgttcataattc
attcttttcccaatgaaaatcaaaggctgccgttaatgattcattgttatagaggcaaaagagacataacagtacagttgagctatttcctccctgaaag
gaactgtagaatgtcttcaatgtatttggactgactcctgtcttctttcagcgaagtaacgtgcaacacacaacgtaacgttgttccatctaacctatac
aatcctt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
