XB-CLONE-3402275
Clone Name: IMAGE:7641707
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Gene:
rnf150
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168034 [+]
agcagggggaaagaaagatggcaatgtctctcatccaagcatgccgcagccttgctctctccacctggctgctgtccttctgcttcgtccatttgctttg
cctggattttaccgtggccgagaaggaggagtggtacacagccttcgtcaacatcacctacaccgatccactaagccaccagtggaggacagagaagagt
gagtgcgggaggtacggggagcactcactcaagcaggatgcctggggcttggcggtgatgccctcttccccgcaggacaggctggcctgtgacccggcca
ccaagttctcagtgccagccaatgccagccgatgggtagcacttattcccaaggggaactgcacatacagggataaaataaagcatgccgccttgcagaa
tgcttctgctgtgctcatttataacgtgggctccagtaatgccaatgaaaccatcaccatgccacatccaggtattgaagatattgtagcaataatgatt
ccagagcctaaaggaagagaaatagccaccttactggaaaggaacattaatgtgacaatatacatcacaattggaacacggaacttgcaaaaatatgtca
gtcggacatcagtggtgtttgtttcaatatccttcattgtcctgatgatcatttctctcgcttggcttgtgttctactacatccagagatttagatatgc
caatgcacgagacaggaaccagaggcgacttggagatgctgctaaaaaagccatcagcaaattgcaagtcagaaccatcaagaaaggtgacaaggagaca
gagccagagtttgataattgcgctgtttgcattgaaggttacaagcctaatgatgttgtccgaatcttaccatgccgacacctttttcataagtcttgtg
tggatccctggcttttagatcatcgcacgtgtccgatgtgcaaaatgaatatcctcaaagctctagggattccgccaaatgcagactgcctagatgatat
tcctcctgactttgaaggttctgttgggcctcccaccaatcagatcacaggagccagcgatgtcactgtgaatgagagttcagtgggaatagacccctcg
gtccgcactgtaggagtgctacaagtcatccaagatgcagaatctctaccgcaggttggggaagttagctatacagcaagcagtgaacaagagccagctg
tcagcagtgactcagacatctccttgatcatggcagtagatgtaggactttcagatgtggatctttctactgaccaagactgtgacgaggcaaaatcatg
agctggagcattgttgctgtctgtaggaaagtaataggagaagaggacaaaagaccagctctcctggagtttggacaaatccaaaaattataaaccaatc
accaccaccataaatctccagcgcactccatcctggtctgatcatgaaaagcaatagcgcccaatgcctgctatgtgctgcacagtttctttctgttggg
ctcccggttgtgttgaacagggagcagagagctccttggttcctcacctgtgcaaggcttccagatgcaggctttcctggtgtggatggtagaacaaatg
gaacgcaaatgaaatctatgatgctgtttaaaaaaaaaaaagaaagcataaaattatatttttttgtttatccagttttaatcagtatattcagtcccca
gtatatgggcgcatcattctatgatccattttatttcaagttcccggcttttcattgtttggcaagtaaacaaggagaggtcataagtcaatactgtgcc
ctaatctgaagacagctatggttactgcttgggcagccttcccagatgcactcaagatgaaatgtcttttagcaacactgcctagcttcatgtgacctgc
cattctccactgactgcataaacaatgtttgctatttcattacatagcagtgtaaggccaacatggtcacgttggcaggatcagccctgattttagactt
caggagaatcatatatgtataaaatatcttccaacctttaaaactgtcagtggatgcccataattaggagcaattaagaaagatgcataaaaacctaaat
ttctgcccaatcccctcaatcacttgcaccccagttgaaaagggaaagctggtgttcttactatacagtagaaaggattcatatattatgcacggttttc
ttccaaactatgtgcatatggactgttgccagataacaccatttcagtatgaccatcatttaccaaccatggcctgttgtgtagaagtgaataggacaaa
ataagaattgactcctgtgtgagggtatacattagatgaacacatgacaggtcatgtgaacctttggtgaaacctgttgacattgtgaacaccaagccca
catccagatctgcccaaagccttcctattttgcaataaaccccatccattttatgtcttgaaatctaggactggcctgggagcaccaggacttttggtag
gtacagattaatttaactgctgatttgaagtatgctgtattggtgcagagtgcaaaaaacacagtgcaccacgtttccaagcatgctatagcaattgcac
ctgctgatatcatgggcaaatcttgtttgaatggtgtgcatgtgagagcaaggttaggccaccacaaatggggcagcacattgttgtatataatatgggt
gctcacttgcacaccacttactagcattccattccataggtgcaaggtgcagcaggagccttgtttttaatttcacctcaaagcaggtataaagtacaag
agtcatatgcctaatgcgccttgcactaagagcatagttaggcacaagtgttttaactatcaccggtttgcaatcagtgcgcctaccctggggtgttttt
ccaaggtaagaacctttcctcaagcagcacctttgcctcacctgttaactttaagaaaagttaacagaaagtcagctcttctagtgcagaaaatgccatt
gcactcccttcactagagatgtgcccagcattggggatgctccagaaatgtccctgttttatagaaaataagtcctttggtgtcagtaccccactgcagc
cacttcctctgtctcccgttattaaacaatgcagattggctataggcagcatgcacatggttaaagccctcatttccctacaattaataccagatgaaca
tcagggggcacaggggaatgacaggagttgcagtgagggttaacaaatatttttatagaaacagtggggtataagtgagtaaaaggatttgcttttattt
tagacttctctgtgtgattttaccttcctggcagattctcctggttctttgttgcgaggctactgtgaaacctatgaaagtgtttctgatcagccactca
tgaacaaaaacatgtttgttttaagcattttataatatggcaactcttactcttacacacacacacaagtaatgtttattagacctagtttcctattagg
ctccttagcattggcaaagaatattatttgtaggtttgatgcaaacattacctctcctgcacagaaagccagcggcccccattagagcagtgatgtgttg
ctttctgtgcgtttgtaagcatgtagtgacgaaggctacaggaatgttggtgggaagaagcaattgcattaatgtcattcacaggaactggctggtatgt
taaaggcatatgcacttgtttataagggtgaattgcataatacttgcttgtttgtcccagattcccaaccggtacatgaaaatatatccaaacacatttt
ttgacctttaatataattttaaaaacaaaatgtatgcagttagctccaaagtagtttcactgtttttccattcatttcactatgtatatttgtctttaat
gcatgtatgttatgcaacatactgtacgtaaacagacatttctaacctggaaaactctgactacttgtgaactacaattctaagcgcttccttatagcaa
gggtattctgggagttgtaatttaacaactggtagataaacatttggacatccctgattttaaacctcattgcctatctccaaatccagaaacccttata
gatatgtgtgttaacatgctgagggctgtgcatagcatctgtgtggggaggtttttttgacagtaggtcagaaggaggcttggtgcttccattgcacaca
catcgttccatcccttccttacctttgttttagctgtaccattctaagagacatgggctcagcagtgcatttagatctaactcactggaggagagaagag
ccctagtgatacaattaccatcatatactgagcagccacagaaagctataatgtgctgtgtattcggtatctaaggaacagtgcattaaccattagaata
ttactgaaagcgtgggcttgtttataaaggcactatattttcttgttccatacattattggtatattagctttcttaaagaatagttaaatatacgccag
acctaacaaatatctgttaggttactaggtcagttactcaaaaaagccagccagccagcccacccctgcacaaaggacaagtgatgtgtgcaaaattgtg
ggtcatggatcacccttgaccagttgtgtgccctctgttccctgggtttccccatgattgttgggtctgcccctgtataattatgccaatgaagtcagct
ttagttattctttcacaccttgaaccagtgcagcaacaatattttggatatacaacagatataagtgcactggaatgtataaggtgtctttcaggcatgg
tgtgttcccaaccagtgaattacacaggtagagtgagttttacatgatcataaaggaaccaaacccatgggtcttattccttgtccattggatacctaag
caaaaaaactccttaacgtgactgatgattgacatttaaaagggggatatatattatagtgtgtaagccaacatcaccttgcccatagcaaccaatgaaa
tctttgcttttgatttctaacttgtaggtgactgtttaaatctaattgctgattggttactatgggcttacacactataataaatatgccccttactgtg
aaacacacagagagggctgctgcttattggtcaaaggcaacactgggtaagtcagagaaacatatttatcaaaggtggaagtaaaaagctttgtaatact
ctgaattcaatttcattcataatatagggctagattcagttcagtgagagaaagtgatcttatggtttaacatgtgaaaactcatggatgagattaagtt
tgaggagaaaatcatttctcctaattgagtttttccaatagaatgtgacaacatttttttacgtgacaaattgcgacaatagattttctgaattgaattg
ctttgcatttcacattgaatctcattcatgagttttcacataaacctttttctcactgaattgaatctggccccttgtgtgagaatttcacttgtttttt
accagtctccctgctcccaactgtgtctgactagggaagaatactgcatgctgggagcaggaagaggtaaccttcccaaccttgctgggcaataacatgg
gcaactttatttagcaaaatgcatgggagctagtgatacaacatgctcctttgcaaatacattaagatgttgtcaagctcattttgacatttgacattaa
tggtcagctgtggccttactcatacactttgtttgtgtgtatctgatagtgttgtttaacggtgctgtgaagcaatgctgctagggtccttctcacatgt
aactgacacaatgcatagattgctgctataaataccaacgatatcctctctagtttctcattctggaagatgccacagataccagtgtatcacgcttcat
aggatacagtgctcagatatgcacttaagcacattgctgtttatagggctatggtacaatggagtagcaccggaaacttaacaccacccctggagaaatc
aatggtgggtggcaggtgatgtgcttccccagcagctggaatccaccagcagagaaaaatgcccttgtgctattagccttaaatgattcctataacaggc
cattgcttattgtccttctcataaacaataccaatatcactacaaggaaggtcttgttttagactctactgttatttttatttcagaatgcctgggatcc
aaatagtctacatgagatgaatttcatgctgtaacagggaaggtctcattgtaacaggtctttcccactctttgactcataaaggttttgtgttgggctc
agtattcagccaaacatttggggaacatttggtatttggctgaatcctaacaatcagtttggtgcatccatcatagttgtcttaaatgccttctgtttcc
aacccaatgttatatttcatgcctctagatagtgttgtactataccctcaactgtccttgttagggaatggtgggagttgtagcttaacaatagctgatg
gcatacgtgttagctgttatttctgttttatcttcttaagggctttatgtacatttacagcatgtgttgcctttctgtaagtcgggtatttaggtccttg
tttagtcttgtatagcattggtattgtttcagctgaatgtgtcatgaaaggccatgcagaaatacttacatagatctatgtgtgatgctttctttatgaa
aggagctgccatgtagcatgcagggcataggaaacatttgcctctgttattgctttacccctatccgaggatgtggagaaagatgcttctctctggtgaa
aaatatcacttacgtactgtatgagaaagcctttctatgcatgtatgtatgtacagtatacacatgccagtgttgtaccattctctattatttccatatg
tttcatttatgtttcatattaaaaattatttttttaaatcaaaaaaaaaaaaaaa
5' EST: CX795525 [+]agcagggggaaagaaagatggcaatgtctctcatccaagcatgccgcagccttgctctctccacctggctgctgtccttctgcttcgtccatttgctttg
cctggattttaccgtggccgagaaggaggagtggtacacagccttcgtcaacatcacctacaccgatccactaagccaccagtggaggacagagaagagt
gagtgcgggaggtacggggagcactcactcaagcaggatgcctggggcttggcggtgatgccctcttccccgcaggacaggctggcctgtgacccggcca
ccaagttctcagtgccagccaatgccagccgatgggtagcacttattcccaaggggaactgcacatacagggataaaataaagcatgccgccttgcagaa
tgcttctgctgtgctcatttataacgtgggctccagtaatgccaatgaaaccatcaccatgccacatccaggtattgaagatattgtagcaataatgatt
ccagagcctaaaggaagagaaatagccaccttactggaaaggaacattaatgtgacaatatacatcacaattggaacacggaacttgcaaaaatatgtca
gtcggacatcagtggtgtttgtttcaatatccttcattgtcctgatgatcatttctctcgcttggcttgtgttctactacatccagagatttagatatgc
caatgcacgagacaggaaccagaggcgacttggagatgctgctaaaaaagccatcagcaaattgcaagtcagaaccatcaagaaaggtgacaaggagaca
gagccagagtttgataattgcgctgtttgcattgaaagnta
3' EST: CX795524 [+]ttttttttcttttttgatttaaaaaaataatttttaatatgaaacataaatgataatatggaaataatagagaatggtacaacactggcatgtgtatact
gtacatacatacatgcatagaaaggctttctcatacagtacgtaagtgatatttttcaccagagagaagcatctttctccacatcctcggataggggtaa
agcaataacagaggcaaatgtttcctatgccctgcatgctacatggcagctcctttcataaagaaagcatcacacatagatctatgtaagtatttctgca
tggcctttcatgacacattcagctgaaacaataccaatgctatacaagactaaacaaggacctaaatacccgacttacagaaaggcaacacatgctgtaa
atgtacataaagcccttaagaagataaaacagaaataacagctaacacgtatgccatcagctattgttaagctacaactcccaccattccctaacaagga
cagttgagggtatagtacaacactatctagaggcatgaaatataacattgggttggaaacagaaggcatttaagacaactatgatggatgcaccaaactg
attgttaggattcagccaaataccaaatgttccccaaatgtttggctgaatactgagcccaacacaaaacctttatgagtcaaagagtgggaaagacctg
ttacaatgagaccttccctgttacagcatgaaattcatctcatgtagactatttggatcccaggcattctgaaataaaataacagtagagtctaaaacaa
gaccttccttgtagtgatattggtattgtttatg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: