XB-CLONE-3398567
Clone Name: IMAGE:7644682
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Gene:
apbb2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161628 [+]
gcactgacagttggacgcacacacattcaagccgttcattagccgagatgagctaaatctccatttgaatacagacagcaaaatagctgcacaaaggtcc
agggtttgatctgtttggagaagcactttgtctgctcccttgagccagttgtcatgttctgaccatccacaatgggcaggcgatatcctggtgcaaccta
ccgaccaagcagttcatatttctaaccctaaccaatatcctgatccattgctgacgcccttctgatccatgtctgaagtcctgcaagtcgattcaagtgt
ggaaacacttccagtttttatggcaagccacagcgccacggacattgtgaatcgaaatgcagttagcccaccaaataccttgaacttgcgctcttcccac
aatgaactattgaatacagatataaagcacgtggaaacgaagagcataactccaccaaaatgcaggaaaaaatatgcactgacaaatatccaggcagcta
tgggcttgtctgaccctgcatctcagcctctcctgggaaacagttctccaaacataaagtttgtaaagaatggggaaaatcagctgaggaaagctgctga
aaatgggaatgggaatcaagaccaaaataaaaatatgagtttagaagatactgtcaacagaatctcagagaaactagaagggaaagaagagatcccagat
gactcagaaagttctgaaattctaccattaattcctagaagaaccaagagttttctcaattactatgcagacttggatctctccattgacctggagcaaa
gcaatgggcatgtagtagaggaggaagactccacatggattaatattcaagaacctgatattcttactaatggggatttgctgccacctaaacagaacaa
tatctctagtcctgaggatagtcagggccctactgtatcatccagcccagagactaagaaagaccacccaaaaactggtgcaaaaactgactgtgctcta
caccgaatccagaacctggctccaagtgatgaagattcaagttggaccacattgtcccaagacagcgcatcccctgtatctccagatgagacagcagata
tatggagtgaccactcgtttcaaactgatccagatcttccacctggctggaagaaaatcaatgatatcgctggcatctactactggcatataccaactgg
agcaactcagtgggaacgcccggagtcagcacctacagatctcccagcctcacgcaaaggatcactcagctctatcacatcaacacccacaccagaaaat
gagaaacagccttggagtgattttgctgttctgaaggggggaaagattaatgttgacatttggaaggatttgcatgcggccacagtcaacccagatccaa
gcctaaaggagtttgaaggagcgacattacgctacgcctcactaaagctaagaaatgtgccgcagtctgatgaagatgattcctccagcattaacagtga
ccctgaagccaagtgctttgcagtgcgctctttgggctgggtagagatggcagaggaagacttggccccagggaaaagcagcgttgctgtgaacaattgt
atcaggcagttatcatacagcaagaatgacatccgagacactgttggcatttggggagagggtaaagacatgtacttgatcttggagaatgacatgctaa
acctggtggaccccatggaccgcacactgcttcattctcagcccattgttagtattagagtctggggcgttggacgggacaacggacgggagagggactt
tgcttatgttgcaagagacaaggatacaagaatattaaaatgtcacgtgttccgatgtgacacaccagctaaggcaattgctacaagtcttcacgaaatc
tgttctaagattatggcagagaggaaaaatgccaaagctacggcttgcagctctatgcaggagaggccccacgccagcctagatgtcccgttgcaagtag
aatttccaacaccaaagacagaacttgtgcagaagtttcatgttcagtatatgggaatgacggcagtctctaaacctattggtattgatatcctgaatga
agccattgaaagtcttttgaacccatcagtcaaggaaagctggactccagttatcatgaatgtagctgatgcaacagtgacagtgataaaagagaaggat
gaagaggtcatagtggaatgcagagttcgcttcctgtcctttatgggtgttggaaaggacattcatacgtttgcctttatcatggatactgggagtcagc
atttcgagacacacgtgttttggtgtgagcccaatgcaggaagcgtctccgaagccgtccaggctgcatgcatgttgcggtaccagaagtgcctggttgc
cagacctccctctcagaaggcacagcctccacctcccccgtcagactctgtgacccggagagttacaactagcgttaaacgcggggttttgtcccttatt
gacactttgaaacagaaacgtcccgttgctgacatgccataattgcaagcgaagggctgaattgtgaacactcgagtataatatcgtaactggaattaca
gtagcgcactcaaggacagcaccgcagtacggccatctttctgctgctttgtctccagagaacttaccctttcccaacgttcgacgagcatgttcttgtt
ctccgacaccatggcgtactgcaaaaagaagcatgaattcaaatatatatctactcataacttttccttttccctgtcagtggttgtcattacatgcatt
ggatagtctgtttcagattgtaaatacgatgaacatttcatgacgagatgagattttctgcactctcctagtagtgaatgccacggagtacttccatgtt
aaggattactcggcatccccctagttcactgcctcatttcatcgctgaaaatgtaataattcattgctgtgatcatgtccacagtggaggtactgttacc
tgatggaaggtgttgccaaagtcactccctggaatccctctaaccagaccccatggcacttaatgattttcatacccaaagaatttgatttctatgaatt
gagggtttcaccttataagcagctttccgggatgttgtaggcagcgctaatctgagccagtttgctctttctctattgggagcttttgacccgcttccca
tttctagtctgtgtttctacaaatatggttgccaagtctagtagcccttacaaacgggaagctgattgaatgtgtacagtaggggaaccccacagcaaca
tttagcccccccaaaacttggtgcaaaaagatatggcagctgcctctaggagttccacagcactttgcgtattatttattggccttccatgctgctcagg
gcagtgtaaccacagaagcttcagagacacagggtccccttattgttacaacctttaatttgagatcagatgcttattttcataaatgatctgtgtattc
atttcagtatgccatttgtactgctgtatgtttgcttgtgtcagtgaccctagcaaccagaagtctatcaaaatgagaactattccaaaaatattaaata
aggaagatcccccagaattctaagggtaccactgcctatggcatattaaaggtgacattcgtgtgcaaggttttacctttaaacgttttatgaaaatcag
atttacctggagaaataggatatatatatttaaatgttctaaaatacatggaatggttttcagttaaacaacttgttgcaccgtagctgtatgattccta
tttcccatggagtaaagggcaagtctgtaggaataccccagttttagactgatacaagtagtttatctgtgctgtacttagtttagcccaaacacaacag
tcctggctcagcgggtttatgtacataagcactcacattatactcattatttttttagatgcataaaaatctgaagagaatgaaactaatattatactat
ggctaaatgcacacagacatgttgtccttgcccagggctgtactaagacccaaggctgtgttgtaaacgctgcagtaaactacagctccaggaagctggg
acatgtagttcaagaacatctggagggcacagtaggtgctaataggggcatcctgttctctcgtacttgtgggcaaaatgcaaagttgcacagtgaagtt
ggtggctgctcaacatactgtcagaggttccacaccctatcagaggttattgttcagctagaacattgataccaaaaactgacctagtactggggagcgg
agctggagtttacttgtttaaattccatgcaaccttgtgaccacaaatccaccagaatttcctaaggcagaatcatggtgctcacaagatttgagccaga
tgctgctgttcctgttatagacaggaagtgtttgagagttgtatggtatggagccatgaatgcctgcaggcaatagacacgcatgttggtcactaccaga
ggacataaatgccagtgcgggaaggggtacattatcccctatatcaataggatacatggttgtgctgaaatagaaattgaatagatctcatgctacagat
ggtaaatatgcccacactgtccttctgttgatgccacataggtcagtagccctattgctttatggcaaacttttgctggttgttagtgcattgtatgtgt
acattatacagggactggcagaaacactatggcactggtaacaaaggaaagtataactgtctttctatagtgtgttggaaatctgtagggagagggacgt
ttggtgtggccttaatacgaatacaggccccctaatgtctgagttggaggaggaaagtcatagtaattcccaaggaaccacttagattgtatgtccagca
acgcaatatctcagagcatcctgtgagaagttgtgtgtgaatttgatgcctatgtattttttgcagttattcagcattctgtcctgtcttatacatgagg
ccccatttggttactgtaactgcccttctattgcgaatggtaatatagtcccacaaattgtcaccagaaaccccttatatagtctcttatatagtaggca
gtaactataaagtgggacattgggagtgattggctgcttgtgagcagtctgggcttagtctttataaaaggaatctgggaatctgtaataattcctggat
atcaccggtatctgcattcagtcaatcaataatccatacattttataagggagggaaatgaatagttttaccttgtcaggggctgtacctgctgaaaaga
ctttctatgattaatggtctcccttcccctggcccccgtgtattggcccccatgtattggcccccgtgtattggccgctcttcactgtttgtgtttgcag
ccaagtgtccttgaaatgagggcaatacactcactgctgtcttctacccgattctactgagaggagacaagctggagagggaaatgctttatactcccat
tatcttacatgggaatgtttaaataggggatacttattgttaattagtacttttccatgtgttcttaatatattggtaatatgatgactttcatattagc
caaatgaagccatcagtgggacatgtctgtgcccattctgtctgccagctggtacaagaaacaatcactcagtcagacaatttggcttctgatctgagag
gctacaagtcatattaagctagctggatcttactagtcacttatgggaatgtccaaatggctcccctaatgggttcagtggccctacattaacttaagtg
cctcgtcccagacatattgtcagttatttcggtcagaatgtaaacaccaccagagaattcagcttgtgaccggcatgatgtaaacgctgccactctgagc
attccccagctaagctgacagacaatgaacagaccctaatacaccgtccagccacaacttccagtcccctcgtgtacatgtccttagttattaacctaga
gctgctaactacaggcagtatggggccgatacccagaagccaggctagaactcatccaaccgcgtggccacttactggcaccctgaaaaatcatgtctcc
atgttgcatggccatttacagctattagggctcacattccgccatagtcatctgtgtttgttttggaaagcgctgcgggaaaaatagggcctattgatcc
cttaatgacttgtaattacccaaacaccagttggacacagcagtttaatttgcagagtgtagcccccagtgatcggtgatactctccctgctctggtgca
tcatgggtatcactatcagtgtagcaggatgggtccagaatgaaacaggaaccctaaaagtgtggcagcaacatattcctagagcactttgtgtttcatg
taagagtcagacattacgggcccttcctatgtgcaacatggtgccagcattaggtgccaatagtagggcacaggatctggctcatattggggcccttcct
tcattgcccaggggctctcatttccataactctggcatcagacgaaatggcggaagtgatctatgggaaaaggaacatcctttgcagatattttgtttta
aaaatagctttgcatattttaacttaaaagattattttgtaaaaccaattgtaattaaaaaagtcacttttttttacggccaaaaagtatgtatgtggta
tgtgtgactaaatgccaacgtgttagtaagagttcatttatctatgcattatttgtatgcagcggctctataaacagccatgttcactaaaaaaaaaaaa
aaa
5' EST: CX801337 [+]cactgacagttggacgcacacacattcaagccgttcattagccgagatgagctaaatctccatttgaatacagacagcaaaatagctgcacaaaggtcca
gggtttgatctgtttggagaagcactttgtctgctcccttgagccagttgtcatgttctgaccatccacaatgggcaggcgatatcctggtgcaacctac
cgaccaagcagttcatatttctaaccctaaccaatatcctgatccattgctgacgcccttctgatccatgtctgaagtcctgcaagtcgattcaagtgtg
gaaacacttccagtttttatggcaagccacagcgccacggacattgtgaatcgaaatgcagttagcccaccaaataccttgaacttgcgctcttcccaca
atgaactattgaatacagatataaagcacgtggaaacgaagagcataactccaccaaaatgcaggaaaaaatatgcactgacaaatatccaggcagctat
gggcttgtctgaccctgcatctcagcctctcctgggaaacagttctccaaacataaagtttgtaaagaatggggaaaatcagctgaggaaagctgctgaa
aatgggaatgggaatcaagaccaaaataaaaatatgagtttagaagatactgtcaacagaatctcagagaaactagaagggaaagaagagatcccagatg
actcagaaagttctgaaattctaccattaattcctagaagaaccaagagttttctcaattactatgcagacttggatctctccattgacctggagcaaag
caatgggcatgtagt
3' EST: CX801336 [+]tttttttttttttttagtgaacatggctgtttatagagccgctgcatacaaataatgcatagataaatgaactcttactaacacgttggcatttagtcac
acataccacatacatactttttggccgtaaaaaaaagtgacttttttaattacaattggttttacaaaataatcttttaagttaaaatatgcaaagctat
ttttaaaacaaaatatctgcaaaggatgttccttttcccatagatcacttccgccatttcgtctgatgccagagttatggaaatgagagcccctgggcaa
tgaaggaagggccccaatatgagccagatcctgtgccctactattggcacctaatgctggcaccatgttgcacataggaagggcccgtaatgtctgactc
ttacatgaaacacaaagtgctctaggaatatgttgctgccacacttttagggttcctgtttcattctggacccatcctgctacactgatagtgataccca
tgatgcaccagagcagggagagtatcaccgatcactgggggctacactctgcaaattaaactgctgtgtccaactggtgtttgggtaattacaagtcatt
aagggatcaataggccctatttttcccgcagcgctttccaaaacaaacacagatgactatggcggaatgtgagccctaatagctgtaaatggccatgcaa
catggagacatgatttttcagggtgccagtaagtggccacgcggttggatgagttctagcctggcttctgggtatcggccccatactgcctgtagttagc
agctctaggttaataactaaggacatgtacacgaggggactggaagttgtggctggaccgtgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: