XB-CLONE-3395874
Clone Name: IMAGE:7647245
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Gene:
phactr1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168492 [+]
tagatgccctcctcttcggtctccttcctgcaccccttccttcccctttggcttttggtggtctctctatattagctcgccgggcgaccctgttcaccgt
tcatccattaatggcggcatcttcagaagaggagactgaccggcgacccatccgaagagtccggtccaaaagtgacaccccttatctggccgaggccagg
atctccctgaacctagagacagctgaagaggtggaaaggctggccgctatgcgttccgagtctctagtcccagggacccacactccaccaatcagacgga
ggagtaaatttgccactttaggaagattgttcaagccttggaagtggaggaaaaagaaaagtgaaaagttcaagcagacatctgcagctctggaaaggaa
gatatctatgagacaaagcagagaagaactcatcaagagaggggtcttgaaagaaatatatgacaaagatggcgacttacataatgaagacggattgatg
gagaatggccaagctgttagcagcggctcctcaatgcttcccatcataacagagctagaatcgacttctatggcaggagatacttgtgcatatgaagtgc
ttccaacctctgaactcatggatgggacagtatctgaagatctgtcgtctgagccaggatcactgtctcaagatccatcatacaagccagccatgctact
gcccccaaaaaaaactgtgggtttcccagtagaccaagaggacacccctgtgaaacaaataacactccttaagcaaccccctgccctgcctcccaaacct
atatccagaattgccaatcatatagcagactccggtgcacctgtgaaactgccttgtatgccagggaaaatgtctcctccactacctccaaagaaagtca
tgatttgcgtgccattaggagggacagactttccacatggtccatattccagccagaagagctcccagcatcatcatactgtgctaccatcccagttagc
agctcatcaacatcagttgcagtatggtagccagcatttgtctacaggatccagctccatacccattcacccatccattcctcctgggtgccgagtgatt
gaagagctgaataagacactcgccatgacaatgcagaggttggaaagctctggtttgcacggaggggagagtatgacaaaatcaggactcagcggctatt
ccgacatgagacaagtgccaacagttgttattgaatgtgaagatgacaaggaaaatgtgcctcatgagacaagctatgatgacgcttcgtgcctctactc
tagagaggacgatgaagatgatgatgaagatgatgatgaagatgatgatagctcactatacacaaattctctggcattaaaggtcttacgaaaagattca
ttggcaataaagttaagcaacagaccctccaagagagaactggaagaaaagaatatccttcccatgcagacggatgaagagaggctggaaacaaggcaac
agattgggaccaagcttacaaggcggttaagccaaaggccaacagcagaagagctggaacaaagaaatatccttaaacctcgaaatgagcaagaggaaca
agaagaaaagcgagagattaagagaagactgacacgcaagcttagtcaaaggcccacagtggaagaactacgagagaaaaaaatccttatacgtttcagc
gattatgtggaggtggcagatgcacaggactacgatagaagagcagacaaaccatggactcgactaacagccgctgacaaagcagctattcgtaaagaac
ttaatgagttcaaaagtacagaaatggaagtccatgaattaagtaggcatttaacaaggtttcacaggccctagcattcagttcagtacttgaatactta
cctattgaaaaaagggaaccacaagtgctgggatacttcatttctcttttacttaaatgtaaatcttcagaactgccttcttttatcaagagaagaaaaa
cagcgtttgcatactctacattgatgacccccatcttcactaaaccttcaggaactgaaggggtgtctaatggatcgtgtcatcagccagcctgtggatc
tgtcttgtctgtacgaactcagcagatgttgttcttcctcgcatctttggacatcagcatctcttgtctcgctgttgtttgtgattcaaaggatagaaca
tgcaaaagattccagagctgattaagtaaaacagacctgtgaagcttacatcagccgtgatgcatcaaagccaaatctacgtaggcttgttagtaagcaa
aaagagagagaacgttgcactagttttcctctgtgaagcattcctggtttataagggtcacatcttccatgcctgtgactggttgttgatttttcagcac
attgtgtcaaatggaatatgtgaaagcctgtcaaaaaaaccaagagggttgagcaaatgtggctgaaagggcaaatgttaatgtagctttgagacatttg
tcaccaacctccttcaatttctcatcttctcagtggtagagtaatcagggtcagtgaattaaaactcatgtcaagcactcattgaacagatacttcagag
ctgcaaatcatacgtcagactaatgtggccccagtcccattaccggaagtctgcagaactctgtatatgattatttaacctgtactgtgcatttcatcgt
ctctccatataacagtcttgcttattctggggccactcctttgcttttttttctcataagcatactgtatgtttgtatcaaaggggcagacttgtcctgt
gatcttttcataccagcctccttacatttctgttcttcaggttctgattgtgtcagtctttgggaatttcatgtcattatattgtgcctgttctgtgacc
gttaagaagcatggctcctatgatgatcagtggtgcttttatacacacctttctgtctggtagagttcaacgctgaaattctatcggcaagagaaagaaa
agcaatagtcactgagcctgtccggattagatggaataacagggagatttctctaccttctattaatgccaacacatgagccacaacttgggatctctta
tttatttctttggaaataaatagaatttcctgttcttccattacctttaatttcattgccataattgttttaacaatgagccttgattcagtgaaaggca
cggtcatttaaggaaccaaccaccgttttgtgttaaaaaattaaagtgccactaaaaataatattgtggtgttgcagggtatatagatccaggttagaag
atgaggtttaagcaaaaagaataatattttaacaaaataaaaagcctccagggaatatattcattgataaactcattgcattcttcacagatgaatgcga
atgctaatacaaaatggcaggttggacaatatttcaaaaagtgtgcgtgtgtgagaatttccagtgtgtgtttagtgatgttcatttctgtgttgtgtgc
tttggaaaacaaatgcaggttgctatttattgcccctcagattcctagtgttagtactgcaggaacactcaccagttatgaaccagccctcgtcgcattt
tgcacttgtgtgtatgtagtggcggctgactagggctgctgctgccacataaaactatatttagccgaaaccttttatcttatggaaccaaacatttcaa
cgtggctgctctgatattgtttgaactcgagttttgtttggaaaagggcccaactattatggtagtgacattatgaactgactgcttaggccagggctct
tccactgaaggctttaggccaggggtggccagactttgtccccgggagatccacttttgactcctccctcaagacgcacgtcgcgtacgtaagcatttcc
attaaacgtgctgcacttccgtatccccaggctttgacgcagtctgcccgaaattcacgggggatcgacttgtggaccgcgatcgacgtattggccaccc
ctgctttaggctgtggctggactagtggtattgctgtgaggtgcttgaagtgctagttcagagatagctgcagaattctaggtgaaacaacaatggttct
gagcgcacccctagattcgtagggggaaaagttatttattaaacctgcttttgtaaatctgagccctacttgtattcatgtataaatctggtattttgag
ataataatgggaatattgttccacctgcttcggaataagtgatggggtgggaaagaggtggtttagcacaaataaagaataataaagctgataggtatca
tacccaagcgatatacatgctatttccagtctgtgctcggggtgtcacagaattctactctctgggtagaactaaggaaaatgaaactaaatgaaaggat
gatgtgtgtttattcattctagtaccttctgccgattcagctctgaagggagaattttggtcaggcgccttctatggcgcccgatcaaaattttctaacc
tggccgatcgacgagccgaccgatttcagcagcttcctgcgacatcggtcggctcgctgacataccatacacgcaccgattatcgtacgaaacgaggttt
cgtacgataatatcggtgcgtgtatggccacctttactggggaagcagcaggtcagtaacgagctgagatgtaccaacaataaaatttggactgtgtaac
aggaatgtcagccaggaatgataaggttaagcagataagtgtctctctgaccaagaataaacagagtaaaggcttctgtagattgcttctgtaggactct
ggtagcactgggtaaaggcaggaagcaagcctgacctggttacaattagtttgatccctaaattcaattctgtcttgctagcactcttaaatatatgaga
aatgctgtttctgtttgcagcaagcagaaaaaaaggctgatatgtccagctggtttaattcctttacacccatgagcactcctttgtttataatttgcat
ttcagtgtagaaatctgtgataaatataccagcaagggacttaaatcataaaaatatggatggtcacacagattgcttaccaacagatgcttctcttggt
acctcatgccttccttggcttggcagggacaatttttgaacttccagtatcaaatactcaaattgataaatgtccctgttgttgggtaagcaaacagaag
aagtacctgccttttttacccccccccctaactttgtgctctagtcacatgcctcttctgcctacccctagtccctgccctggctgtcacactatatact
acaaaaggctacacaatatcattattgcttggatgcaattttgctaagtttaatattgagccttcataacccctgttattgcatttagtggatttagcca
ctagttcaattctgtctctaaactgagccatgtgttttcctaatatttctctgtaatgttacatatttttaaatatagcagggagtggtgtgattataat
tgatgcctaaggaaatgtctgagcacctaaacttgttttttttctttattagaaaggcaagtttatttggcactggtgtgtagttgtgagcagattgtgt
aaccttgggaataaccttgatgctagaggttacacagaagaaagggtactttttccacactagcccctaccattcctctgctgtttaaatacatttaata
cgtaaagggcaataagaattacaaaatctttttgtagcctcaaattcagtgttgagcaagcactgcagggaggatggtggctacagtctgcccgtgagta
gcccgaagtggacattttgggaaactgatgaatccattatcctgtaggtgtgggttgcttttaagtgaggcacaagatgagcaaacttccgtatgagtga
ctgatgggagggttttattgtcattgttcattttaaggtcactatttttatttgtatcgatttgtgttttactaagggagacatgaagcacttatatgtt
ctgcggaggtaagctggatgaactatttcagtgaataaagaataatctataaaaaaaaaaaaaaa
5' EST: CX805589 [+]tagatgccctcctcttcggtctccttcctgcaccccttccttcccctttggcttttggtggtctctctatattagctcgccgggcgaccctgttcaccgt
tcatccattaatggcggcatcttcagaagaggagactgaccggcgacccatccgaagagtccggtccaaaagtgacaccccttatctggccgaggccagg
atctccctgaacctagagacagctgaagaggtggaaaggctggccgctatgcgttccgagtctctagtcccagggacccacactccaccaatcagacgga
ggagtaaatttgccactttaggaagattgttcaagccttggaagtggaggaaaaagaaaagtgaaaagttcaagcagacatctgcagctctggaaaggaa
gatatctatgagacaaagcagagaagaactcatcaagagaggggtcttgaaagaaatatatgacaaagatggcgacttacataatgaagacggattgatg
gagaatggccaagctgttagcagcggctcctcaatgcttcccatcataacagagctagaatcgacttctatggcaggagatacttgtgcatatgaagtgc
ttccaacctctgaactcatggatgggacagtatctgaagatctgtcgtctgagccaggatcactgtctcaagatccatcatacaagccagccatgctact
gcccccaaaaaaaactgtgggtttcccagtagaccaagaggacacccctgtgaaacaaataacactccttaagc
3' EST: CX805588 [+]tttttttttttttttatagattattctttattcactgaaatagttcatccagcttacctccgcagaacatataagtgcttcatgtctcccttagtaaaac
acaaatcgatacaaataaaaatagtgaccttaaaatgaacaatgacaataaaaccctcccatcagtcactcatacggaagtttgctcatcttgtgcctca
cttaaaagcaacccacacctacaggataatggattcatcagtttcccaaaatgtccacttcgggctactcacgggcagactgtagccaccatcctccctg
cagtgcttgctcaacactgaatttgaggctacaaaaagattttgtaattcttattgccctttacgtattaaatgtatttaaacagcagaggaatggtagg
ggctagtgtggaaaaagtaccctttcttctgtgtaacctctagcatcaaggttattcccaaggttacacaatctgctcacaactacacaccagtgccaaa
taaacttgcctttctaataaagaaaaaaaacaagtttaggtgctcagacatttccttaggcatcaattataatcacaccactccctgctatatttaaaaa
tatgtaacattacagagaaatattaggaaaacacatggctcagtttagagacagaattgaactagtggctaaatccactaaatgcaataacaggggttat
gaaggctcaatattaaacttagcaaaattgcatccaagcaataatgatattgtgtagccttttgtagtatatagtgtgacagccagggcagggactaggg
gtaggc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: