XB-CLONE-3393303
Clone Name: IMAGE:7649258
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Gene:
ugt8
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121238 [+]
AAAACATTCCTCAGTCATGGTGGGTTAAATGGTATTTTTGAGGCTATGTACCATGGAGTGCCTGTAGTGGGGATCCCACTGTTTGGTGACCATTATGATA
CCATGACTCGAGTACAAGCCAAAGGTATGGGAATTCTTCTTGAGTGGAAGAAAATGACAGAGGATACTCTGTTTGCATCGCTAACTGAAGTTATTCATAA
TCAAAGTTACCAACAACAGGCAGTAAAATTGTCTACAATTCACAAAGACCAGCCGGGCCATCCAGTGAGCAGGGCAGTATATTGGATCAATTACATACTT
CGACACAATGGAGCTGACCATCTGCGTGCTGCTATTCACAACGTCTCTTTCTATCAGTACTTTTTACTAGATGTAGTATTACTTTTACTTTTTGTTGTTA
CTATAGCCTGGTACTTCATTTCAATGCTAGCGAAATACATGCGGAAAAGCATGAGAAGCAAGGAACACGTCGGCCTCAATGGGTACTATCAAAATGGCAC
AGCAAATGGCAAATATAGGAAAAATGGGCACATAAAACATGAAAAGAAGGTGAAATGAGCCTGGGGAGAATTGAGTTAAAGTATGAGGTTAAATTAGTTG
ATATTAATTCTTTTACAAATGCTGGAGAAGTCTTGTTTGCATTCTTGGAAGCAATTAAGTCAGCAGGCGTGAGTCATGAGAATACTGTTTTGCTACACTT
TTCAAGCAAAGTTTTTCTTTTTTTCATGTGGCTCCTTCTGACCTCATAAATCCAAGCCAATGTACTTTAGACTCTCCTAACACACGAAAAGCTTAAAGCG
GGGTTTCCATTAACTCTTCTAAGCACTCCCTCACTACTACATACCATACCATACTAAGTAATGAATTTGATTTCCTTAACAAGGAAATGTTTATTCTGCT
TCCTTTTGTTTAACAGAGATGTGTTATTGCTTCCAAAAAATGAACCAAACACAAACCTAATTTTGTTACAATATATATATATATTTTTATGTGAAATGAT
ATTTCACATATTTCTCTTGAGTACACTCTTTAGAAAAACAGAAATGTCATTATAAATATAGTATCAATAATTTCTTTTTCCAGGAAGCCCCTAAACGTAT
GTTAACCACAGATCCAAAATATTTTCTAATGGTATTTTTTGCTAAATGTTTGATATTTTTGAAATTTTTTCCTGTTTTCTGGAATTAGGATATAATGGTA
ATATTAACTTTCTCATTGTAAATAATTGGCAACGTATTGTATTTTAAGGAAATACAATTATTTTTTAGCTTTAGCACAATGCTTCATTGTGACATCAGGT
GCTGTTCCATATGGAAACATTAGTGCAGGATACTTTCAGGAGTACACAATCCATCAGTTATTTGATGTTGCAACCCTAAACGGTTTTGTTCTTCACATGT
TAAATATCTTGGTATTGTTAATAGCTTTGATTTTGACAGGGGAAGCAAGAAAGTCTTGTTGAGTAATCCATTGTACAATATATAGTTAATATTTTGCATT
TTGTTTTCTTCAGTTTTTGTACCACTTAAAAACCTGTATGCTTTTTAATTTTGAAGAATGCCAGTTCTTGTAAAAGCAGTTCAAAAAAGGTTCTACAATG
AAACCTGCTGTTTTCATAAATATTAATGTATTCTGATCTCATATGCAGTTATTTTTCTTAGATACCATTTGTCGCACTAAATGTAAGGCAGGCATTCATG
CAGCCCACATTGTGACATCTCCTCATGCCTGATATAATGAAGGCTGACTTTATTTGATATCAAGGAGCCACATCATTTATGCTCAGTTTTTGATTTCATA
ATCCCTCCCAATTATTGTAACTGAGACTGAGCATATATAGGGAGATTTGATTATAGAAACAAAAATAAGATTAGAACAGTTAAAGTACTATGGTAGAAAC
ACGAGCTGTATTCTTTCCCTGTGTTTACTGCAAATACCTGGAATTTTAGCTGTACATATTTTGCATGATGCATGTAGATAGTGAGGATAACTTATTTATT
AATGAAATGGAAAGAGACAGTGTTATTTCCTTCAAGAGCTACATGATAATATGTAATTGAATATCCAGGTACTTAAACTAAACGTGTACAATAAATATAT
GTGATAACCTTCTGTTGTTTGTCTTCCTGTTAAATTTAAATTACAGGGTTTCCCCCATTTATATCTACACATTAGAATGTCTGAGAATAATTTATTTTAT
TTTTGGAATGTTTTACTGATATGATATACAGAGAGAACCACATCCTTGTTTACAGGTGAAGCATTGGTCTATTTACGATCAATAGGAAACAAAGAAAGTA
ATTCTGTGCATTTGTAAAGCTAAAGTCTGTCCTACTTTGCCTCAAAGGTTAAAAATGACTGGAATTGACTGGAGTGTTTCATAATGCAATAAAACAGGCC
TTTAAAAATTAGGGTGATGGGTCAGTCAGAGTGACTCAGTGATATATTTGTGTGTTAGATATGAAGGAGACATCCTGCAGTAAACATGTAGCAATGACTG
GAGGAAGAGAGGTGGCTCATGGCGTGAAAAAGCTGACAGTATTGAACGTGTATTTGTTATTTAAGAAGACCTTACTCATTTTCACAGTAGTTGTTTATAT
AATACTGGACAATGAACGGAATGTGTAAAGGCACTAATACCAGTGCCATATGTTTATATACAAACGTACACATTAATCACAAATATACACACATCTCACT
AATCTGAATGATTACTGACTTCTCAGACTGTACTTGTATGTCGATGTACAGTTTTTTCTGAATCCATGCACCAACGTTTTTTGTTAACAATTAAAAACCT
ATTGATTGAAAAACACATGAGATGGAAATGTATAGATTCTTGCTATCTTCATTTGTCTGACAGCCATGATGGAAAAGTAAACAGGAACAAAGTATCGTTT
TACTGTGTACATCATTGGATGGTTTTAAATACAGTATTGGCAAAAGTTTTAATCTTGTAACTAATATGGATATCTTTTATGAATAACTGTAAATCATGGA
ATCTTAGAGAACACACTACGGACACTGGACTCATTCACTTAATACAAAAGTTTTATTGCAATACAATACCATAATAATAATAATATTGGGAAAGGGAATA
TATGGTTTACCTTAATGCCTTAACATCCAGTTTAGATGTTTCTCTAAAAAACATGAATTTTGTTTCTCTGAATCATGCTTTTATGTTTGTTTAAATATTT
GAAATAAAACCCCTTTAAATATAAAAAAAAAAAAAAA
5' EST: CX809657 [+]aaaacattcctcagtcatggtgggttaaatggtatttttgaggctatgtaccatggagtgcctgtagtggggatcccactgtttggtgaccattatgata
ccatgactcgagtacaagccaaaggtatgggaattcttcttgagtggaagaaaatgacagaggatactctgtttgcatcgctaactgaagttattcataa
tcaaagttaccaacaacaggcagtaaaattgtctacaattcacaaagaccagccgggccatccagtgagcagggcagtatattggatcaattacatactt
cgacacaatggagctgaccatctgcgtgctgctattcacaacgtctctttctatcagtactttttactagatgtagtattacttttactttttgttgtta
ctatagcctggtacttcatttcaatgctagcgaaatacatgcggaaaagcatgagaagcaaggaacacgtcggcctcaatgggtactatcaaaatggcac
agcaaatggcaaatataggaaaaatgggcacataaaacatgaaaagaaggtgaaatgagcctggggagaattgagttaaagtatgaggttaaattagttg
atattaattcttttacaaatgctggagaagtcttgtttgcattcttggaagcaattaagtcagcaggcgtgagtcatgagaatactgttttgctacactt
ttcaagcaaagtttttctttttttcatgtggctccttctgacctcataaatccaagccaatgtactttagactctcctaacacacgaaaagcttaaagcg
gggtttccattaactcttctaagcactccctcactactacataccataccatactaagtaatgaatttgattt
3' EST: CX809656 [+]aaaggggttttatttcaaatatttaaacaaacataaaagcatgattcagagaaacaaaattcatgttttttagagaaacatctaaactggatgttaaggc
attaaggtaaaccatatattccctttcccaatattattattattatggtattgtattgcaataaaacttttgtattaagtgaatgagtccagtgtccgta
gtgtgttctctaagattccatgatttacagttattcataaaagatatccatattagttacaagattaaaacttttgccaatactgtatttaaaaccatcc
aatgatgtacacagtaaaacgatactttgttcctgtttacttttccatcatggctgtcagacaaatgaagatagcaagaatctatacatttccatctcat
gtgtttttcaatcaataggtttttaattgttaacaaaaaacgttggtgcatggattcagaaaaaactgtacatcgacatacaagtacagtctgagaagtc
agtaatcattcagattagtgagatgtgtgtatatttgtgattaatgtgtacgtttgtatataaacatatggcactggtattagtgcctttacacattccg
ttcattgtccagtattatataaacaactactgtgaaaatgagtaaggtcttcttaaataacaaatacacgttcaatactgtcagctttttcacgccatga
gccacctctcttcctccagtcattgctacatgtttactgcaggatgtctccttcatatctaacacacaaatatatcactgagtcactctgactgacccat
caccctaattt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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