XB-CLONE-3393214
Clone Name: IMAGE:7649711
|
Gene:
pi4kb
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC135973 [+]
GTGAGAGGAGCTCGGGATGGGGCTGGGACCGGCCTCTGTGACCGCTGAACCCGGAGACGTAATGGAGCTCTGACTTGAGGCCTGAGCCTGAGATGGGAGA
CACCATGGTGGAACCAGTACCTGCGAAGCTCTCCGACCCGACCCTGGTGTTGCGGGGCAATGGAGGGAGTCCCCTGTGTGTGATTACGGAGGGCGTGGGG
GAGGCCCAGATGGTTATAGACCCGGACGTGGCGGAGAAGGCCTGCCAGGATGTGCTGGACAAGGTTAAGCTGATTCGCGGTTCCTCGGCAGAGAGCTTGG
ACAAGATAGATGGTAGCGACACTGGCGATGGCGGTTCCCTGGCCAATGGGGACGCGGGGCCCAGACACAGCGAGAGCTGCGGGCCCCCTGTCAGCGCCTC
CAGGATTACTGAGGAGGAGGAGTCACTGATAGACATAAACAGTGTAAAGAGTGCCCGCCGGAGGCAGAAGAACAACTCGGCCAAGCAGTCGTGGCTGCTG
CGGCTCTTCGAGTGCAAGCTGTTCGACGTTTCCATGGCCATTTCGTACCTGTACAACTCCAAGGAGCCGGGGGTGCAGGCCTACATCGGCAACCGCCTCT
TCTGTTTCCGCTACGAGGACGTGGACTTTTACCTGCCGCAGCTGCTCAACATGTACATCCACATGGACGAGGACGTGGGCGATGCCATCAAACCCTACGT
GGTCCATCGCTGCCGGCAGAGCATCAACTTCTCCCTGCAGTGCGCGTGGCTCCTCGGCGCTTACTCCTCCGACATGCACATTTCCACCCAGCGCCACTCC
CGCGGCACCAAGCTGCGCAAGCTGATCCTCTCCGACGAGCTAAAGCCCGCCCACAAGAAGCGAGAGATCCCGCCCCTCAGCCTGGCCCCAGACACGGGCC
TCTCGCCTTCCAAGAGGACTCACCAAAGGTCCAAATCCGACGCCACCGTCAGCATTAGCCTCAGCAGCAACCTGAAGCGGACGTCCAGCAACCCCAAAGT
GGAGAACGACGACGAGCCGGTGCGCCTCGCTCCCGAGAGGGAATTCATCAAGTCTCTCATGGCCATCGGAAAGCGCTTGGCCACCCTTCCCACCAAGGAG
CAGAAGACCCAAAGGCTCATCTCTGAACTGTCCCTACTGAACCACAAGCTGCCCGCCCGGGTCTGGCTTCCCACTGCCGGCTTCGACCACCACGTGGTGA
GGGTGCCACACACGCAGGCCGTGGTACTGAACTCCAAGGATAAGGCTCCCTATCTGATCTACGTGGAGGTTCTGGAATGTGAGAACTTTGAGACGTCTCT
GGTCCCGGTCCGTATCCCTGAGAATCGGATCCGCAGCACCCGCTCTGTAGAGAACCTCCCCGAGTGCGGCATCACCCACGAGCAGCGCGCCAGCAGCTTC
ACCACTGTCCCTAATTACGATAACGATGACGAGGCCTGGTCTGTGGATGATATTGGGGAGCTGCAGGTGGAGTTGCCGGAACTTCACACCAACAGCTGTG
ACAACATCTCCCAGTTTTCGGTGGACAGTATCACGAGCCAGGAGAGCAAGGACCCCGTGTTTATAGCCGCCGGCGACATCCGGAGGCGCCTCTCAGAGCA
GTTGGCACACACCCCGACTACATTCCGGCGGGACCCTGAAGACCCCTCAGCTGTGGCCCTTAAGGAACCTTGGCAGGAGAAAGTCAGGAGGATTAGGGAG
GGCTCCCCCTATGGGCACTTCCCAAACTGGCGGCTGCTGTCGGTCATAGTGAAGTGCGGGGACGACCTGCGCCAGGAGCTGTTGGCCTATCAGGTGCTAA
AACAGCTACAGTCCATCTGGGAGAGTGAGCGCGTGCCGCTGTGGATCCGCCCGTACAAGATCCTGGTGATCTCGGCAGACAGCGGCATGATTGAGCCCGT
GGTGAACGCCGTGTCCATCCATCAGGTGAAGAAGCAGTCGCAGCTGTCGTTGCTGGATTACTTCCTGCAAGAACACGGCAGCTGCACCACCGAAGCCTTC
CTCACAGCGCAGCGCAACTTTGTGCAGAGCTGCGCCGGCTACTGCCTGGTGTGTTACCTGCTGCAGGTCAAGGACCGGCACAATGGCAACATCCTGCTGG
ACGCCGAGGGCCACATTATCCACATCGATTTCGGCTTCATTCTGTCCAGCTCCCCCCGCAACCTCGGCTTTGAGACCTCCGCCTTCAAGCTCACCTCTGA
GTTTGTGGATGTGATGGGGGGGCTCAACGGGGATATGTTCAACTACTACAAGATGCTGATGCTGCAGGGACTCATCGCTGCCCGGAAACACATGGACAGG
GTGGTGCAGATAGTGGAAATCATGCAGCAAGGTTCCCAGCTGCCCTGCTTCCATGGCTCTAGTACAATCCGGAACCTGAAGGAGCGCTTCCACATGAACA
TGACGGAGGAGCAGCTGCAGGTCCTGGTGGAGCAGATGGTGGACGGCAGCATGAGGTCCATAACCACCAAACTGTACGACGGGTTCCAGTACCTGACCAA
CGGCATCATGTGACCGCCGCCCGGATCCGCCACGGTTTCCCATTAACTAAACTATTTTTGTAACTGTTGAGCGGGGACATAATGAGCCCCCACAGCACAT
TGTACAGGATCATGGCTTAGAATGAGGTTTCTCCTTTGGGGGGTGCTGGTGGGTTGCCCTGTCCCTCCCTGGCTGCAGCACTAGGCCACGGTGACCACTA
TGCAGGCAGTTGCCCCCGCCCCCACCGATTCATTCAGTATTTCCACTCAGTGTTTGGCCCATACCATCATGCTCCTCCCACCAGCCCCTTTCTACTATAT
CCCTTCCCCCATGTGACTCTTTCATTCCACTTCTCCCTCATGTGATTATGCTCCTCCCACTTAGCCCTGCCTAAGTCCCATTCCTCCCCCATGTGATCTT
GCTCCTCCCACTTAGCCCTGCCTAAGTCCCATTCCTCCCCCATGTGATCTTGCTCCTCCCACTTAGCCCTGCCTAAGTCCCATTCCTCCCCCATGTGATC
TTGCTCCTCCCACTAGGCCCTGCCTATGTCACATTGTCCCCCATGTGATCATGCTCCTCCCACCAGGCCCCTGGTCTTTGTCACATTGCTCCTCCCACTG
TGTGATTATACTCTTTCCTCTAGCCCCGCCCCCATTATCATGCTTCTGCCCCAGCTCACGTGTCTCCCATAATGCCCCTCTGTGATCATGCTTGTCCCAC
CCAATCCGCTTACTGCTCTAATGTGCACCATGTGGTTATTTCCCTCCCACGAGGTCCAGTCTTTGCCATAACACTCCCCCATGTGCTCTTGCTCCTCCCA
CCAAGGCGGGGGCAGTTCTCCAGACCTCATGACATACTTTCTTACTCTGTGGAAGGGGTTAATGCTAAACTGGAGGGGAGGGTGATGCAGATTAACCCAT
TGGGCTCCCACTGGGCTCCAGGGATCTTTATATTGTAATGTGATATCTGCTAGCCCCGCCTACCTGTTGGCCCCACCTACATACCTACATGCATCTAATT
GCCCCATAGCGCCGAGCTCTTCTGCACACACCCCACTCCCCAAAGGGTTTTTATAGTCTCTAATGCACCACTGTTAGCTCCCTGCTTCCTTCCCTGCCCC
CTGCTGGGCAAGCAGCTGTATGGCAGCCCCGGCCGCCCTGCCTGTGACTAGGAGGGGGCAAGTTCTTCCTGTATATGGAATCCCCTCTGATTATTCTCTT
TTATTTATATGATTTTATTAGGAAATGTATTGCTCTCTGTAACGCTCTGCTGCCCCCTGTTGGGGGCCCCAAGGGCAGCCTTTATAGGGCCCCTCTCCCT
GTGCCCCCAGCAGACACTGATTCTGTGGCAACCGGACTGTAGTTGCACCGGGGCTTGGGGTAAATGGAATGTGTTGGGGCAGAAAGACTTTTACCTCAGA
GAGGAAGTGGCCCCCCTGCTGATTTAGGGGCGGGGAGAACATGGGACTCCTGTTTATTAAGGGGGGGGGGGGACTTTTTAAAAAAAAAATGATAACGTCT
GAGCGCTTTCATGTTTATTTCTATTAAAAGTGATAATTCCCAAAAAAAAAAAAAAAA
5' EST: CX809814 [+]tgagaggagctcgggatggggctgggaccggcctctgtgaccgctgaacccggagacgtaatggagctctgacttgaggcctgagcctgagatgggagac
accatggtggaaccagtacctgcgaagctctccgacccgaccctggtgttgcggggcaatggagggagtcccctgtgtgtgattacggagggcgtggggg
aggcccagatggttatagacccggacgtggcggagaaggcctgccaggatgtgctggacaaggttaagctgattcgcggttcctcggcagagagcttgga
caagatagatggtagcgacactggcgatggcggttccctggccaatggggacgcggggcccagacacagcgagagctgcgggccccctgtcagcgcctcc
aggattactgaggaggaggagtcactgatagacataaacagtgtaaagagtgcccgccggaggcagaagaacaactcggccaagcagtcgtggctgctgc
ggctcttcgagtgcaagctgttcgacgtttccatggccatttcgtacctgtacaactccaaggagccgggggtgcaggcctacatcggcaaccgcctctt
ctgtttccgctacgaggacgtggacttttacctgccgcagctgctcaacatgtacatccacatggacgaggacgtgggcgatgccatcaaaccctacgtg
gtccatcgctgccggcagagcatcaacttctccctgcagtgcgcgtggctcctcggcgcttactcctccgacatgcacatttccacccagcgccactccc
gcggca
3' EST: CX809813 [+]ttttttttttttttttgggaattatcacttttaatagaaataaacatgaaagcgctcagacgttatcattttttttttaaaaagtcccccccccccctta
ataaacaggagtcccatgttctccccgcccctaaatcagcaggggggccacttcctctctgaggtaaaagtctttctgccccaacacattccatttaccc
caagccccggtgcaactacagtccggttgccacagaatcagtgtctgctgggggcacagggagaggggccctataaaggctgcccttggggcccccaaca
gggggcagcagagcgttacagagagcaatacatttcctaataaaatcatataaataaaagagaataatcagaggggattccatatacaggaagaacttgc
cccctcctagtcacaggcagggcggccggggctgccatacagctgcttgcccagcagggggcagggaaggaagcagggagctaacagtggtgcattagag
actataaaaaccctttggggagtggggtgtgtgcagaagaactcggcgctatggggcaattagatgcatgtaggtatgtaggtggggccaacaggtaggc
ggggctagcagatatcacattacaatataaagatccctggagcccagtgggagcccaatgggttaatctgcatcaccctcccctccagtttagcattaac
cccttccacagagtaagaaagtatgtcatgaggtctggagaactgcccc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: