XB-CLONE-3391687
Clone Name: IMAGE:7650730
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Gene:
arhgap33
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC133722
[+]
GACATGGAGTCGGAGGCAGTAGGGGTGGCTAAGGAGGTTTGGGGCGGCTGATTTCCCAGAGAGAAGGATGGAAGGGAATTCTCCCACCCCAGTGACACAG
GAATCCTGATATCCACTGTCTGAATTGGGACAATGTACTGAGACAGGCTGTCAGGGAAAGAGACATACAGAGAGCGTCTGGGAAAGCGTTCCAGGGAGAC
GCCTGGCTGGGCACTATGGGAAGGCAGTGCGGCGAGGAGACCATGGCTGGGGGCAAAATAAGGGGCACCTAGGGGGCAACTGGGACCCACTGGAACCCAA
GAGCTCACACAAAGGCAGTTAGTGGGACGGCGCGGAATCACCAGGGAATTGTGTGGATTTTGTCGCCTTCCAACATCGTGGGAGATGGAGTCCAGCGAGG
GATAATTACACCATCATCCTCTGTGTGAGGCAACAAAATGGTGGCACGTGGCACGGACACTCTGGATTCAGCCAATGAAAGTCGCCTCCTTTCCTCCGCA
GCCAATCCCGGTGTTAAAGGAAGACCAAGCAAAAGGATCACGCGAGGTCCTTTCGTAAAATTGGCAGATTGTGCCCATTATCACTATGAGAATGTCAACT
ACGGACCAATTCAGCTCGGCCTGTCTGATTCCCGCACTGAGGATGCCGGTAGCACCGAGGGGGATGTCATCTATATTGTACAGGTCACATGTCAGGGACG
TTCCTGGTCCGTTCTGCGCAGTTATGAGGATTTCCGGGTGTTGGACTCCAACCTTCACCGTTGTATCTTCGACCGGCGCTTCTCCCAACTGCTGGAGCTG
CCCCCCCGCTCTGAGCTGCCCGCCCAAGGCCAGCTCCTCGCCCCTCTGCTCTCTCGGTATCTGAAAGGTCTCACCGGAATTGTTGACAGTAACATCAACT
GTGGCCCCATACTGAACTGGATGGAGATTGATAACCATGGCAACCGGCTGTTGGTAAACGAAGAAGCGTCCATCAACGTCCCTGCCATTGCCGCTGCCCA
CGTGATCAAGCGCTACACAGCACAAGCCCCCGACGAGCTGTCGTTTGAGGTTGGGGATATTGTGTCGGTGATTGACATGCCCCCCAGGGAGGACACCAGC
TGGTGGAGAGGGAAACACAGCTTTCAGGTGGGTTTCTTCCCCAGTGAGTGTGTGCAGCTCATCACAGACAGTAAGAGCCCCTCTCCCACAGGAGTGTGTC
GGCGGCACGGGAAGCTGGCCGGTCTCTTGCGCAGTTTCATGGCGTCTCGGCCCAGCAAGCAGCGCCTGAGGCAACGGGGCATCTTGCGAGAGAGGGTATT
TGGCACCGACCTGGGGGAGCACTTGCTGAATTCCGGAGAGGAGGTTCCCAGGGTGCTGCTGTGTTGCGCTCAGTTCATAGAGAAGTTTGGGGTCGTGGAT
GGGATTTATCGCTTGTCGGGGGTCTCTTCCAACATCCAGAAACTGAGGCACGAGTTCGATAGTGAGCGGATCCCGGATCTCTCTCGGGACACCTTCCTGC
AGGACGTGCACTGTGTCAGCTCTCTGTGCAAGTTGTACTTCCGCGAGCTCCCCAACCCGCTGCTCACCTACCGCCTGTACCAGCCCTTTACTGAAGCCAT
GTCAGCAGCCACCGAGGAGGACAAACTCATCCGTGTACATGACCTCATCCAGCAACTCCCACCCCCGCACTACAGGACACTCGAGTACCTGATGAAACAT
TTGTCCCAGTTAAGCACCCACAGTGACCGTACGGGGATGCACGCCCGCAATCTGGCCATCATATGGGCTCCCAACCTGCTGCGCTCACGGGACATGGAGT
CAGTGGGGACGCCGGGGGCGGACGCGTTCCGCGAGGTGCGAGTGCAGTCAGTGCTGGTGGAGTTTCTGCTGTGCAACGTGGAGACCCTCTTCAGTGACAC
CTTTACTTCTATTGGTCGTTGCGGCCCAATTAGGCCTCGCTCCCTGTTGGGCGTCTGTCCATCAGGGCGTCTGGTAAGCTTGCAGGAAGCTCAGGCTCGG
AGCCAGAGCCAAAGAGAAGCTGCCGAGAAGCTGGGCGCAGAGAAGGCATCTCCCACCAAGGCCGAGAGGGGGCTGGAGAGCAGTGAGGAAAGGAAGGCCG
CGCGTGGCAGAAAGAAATCGGGAGGCGGCAGCAGCTGGAGAACTTTCTTTGCAATTGGGAAAAACTCCTCACACAAGAAGTCTCGCCCCGGGGGCATAGG
GAATGCGGCAGCCCACCCAAGAGTCTCGGACACGGTCACTCTGCGCTCGGCTAAAAGTGAGGAGTCTCTGTGCTCTCACACCAGCGGAACCGGATTGCAG
AGGTCGTCACGGCTCCGCCGGCCCAGATCCACAAGCGACGGCCTCACCACCCGGCTGCCACACAGTCGCTCTGTGGAGAGTTTGGGGCCCTCCTCCCCTT
CCCCTCCCTGTGTGCCGTCTCCCCCAGCTTCAGCCCGGCCCCAGTCTGCCTGGATAGAGGAGGAGCTTGACCTGTCCCCCCCTCTCCCTGACACAGATGG
GTTGGGCTTTGACCCCCTGTCCTTCCGCCTCTCTTTCTCTGACCATGAAGAAGCCAAGTCCCCCATTATAGTGAGGGCGGAGATAACGAGTCCGGCGCTG
GCTAAAGCCCTCCCCCGAGGAGTCAGCCTGTCCCCCACAGCTGGCTGCTCCCCCTCCCCTACACACAGCCCCCCAGAGAGACTGCTTGTGGATGGGCCAA
GCCAGGGGCCACGTGACCTGTCCCCCCCACCCCAGATGCTGTCCCTCCTGCTCCAGTCCTGCCAGCAGCACCTCAGTGATAAGAGTGTGAGAGAGGTGAA
AGGGAAACTGACTGGGGCCCCCGGCCAGGAAGAGCCCCCCAATAGTCTGTCTCCCCAGGAGTCTGCGCAGCCTCCTTCACTGTCTCTTCTGCGCCAGCTC
CCCCCTCCTCCTCCTCCTAAAGATCCGGCGCGGCTGATGGCCCTGCGGCTGGCAGAGAGTGCCCAACGTGCCTTGCAGGCCTCCATCTCGATAGGCACAG
TCCCAGCCAAGGTGTCCCAGCGCTCTGCGCCCATGAACCCTCCCACCTCCGCATTCCGACGCTCTCTTTCCATGGAGTGCAGTGAGAGCGCTGTGCCGAG
GGAGCTGTACTCTGATCTCCGCCCCCCTGCACTGAGCACCAATGACAAGAGCGTCGGACAGCACCGGCCTTTGAGACTGCCCGCCCACAATACTCTGGCC
CCCAGTTCCTCTCCAGCCGATGTCAGGAAGCCGACGCCTACAGGAATCCCACCTACTCGTGCCCCCCGTCTTCACACGCGTTCAGGAAGCCTCCCTGCTC
CTCCGAGCCTGTGCCCCCCCAGAATCCGACCTCCCCCATACCGATTCCCCGAGGAAGGACCCCGGCTGCCCCACCAATGTTCAGCTCCACCTTGGCCGGA
ACCAGAAGGTCTTTACTATGAGATTGTGGGGGACCCTCCTCATATGGCCCCTCTTAATACGTCGTATGGTCTGATCCAGCCGCCGTGGCCTCATGTGGGC
TCCTCCCAGGCCCATTCGGGTTTTGGGAACTGGTACGGAGCAAGTCATGGTACTGACCCAGAGGTGGTGGCCTATTCACGCTGGGACCGGGGCCTCCATC
CTCAGCTTCGGCCTTACATGGTTGGGGGGGGAGTCCACTATCCGTACGTGAGGACCCCGGAAAGTGTCGGGAAACGAGAGGAACCGATATACGTCAACCT
TCCTCTCAAGGGGAGGGAGCAGCACGACGGAACCAATGACGTCGCAGGGTTGAAACCCGAGTTGCCCCAGAAACAGCGGCCCCCCAATCCCCCAAGCATG
GCGTATCACGAGAGCATCCGAGGAGTAGCCCAAAGGCAAGTGAGACACCCCCACTTACTGCGGAGAGAAGGTTCCCTTGGATACTTCCGCCCCCCTTCGT
CTCACAAAGCAATGTGGGGGACGCAGCATAAATTCCCCCAGGGCAGAGAGAACCCCATCTTTGCTTACGAGGCTCCGACAGGAGCGGGACAGAGGATGAG
ATACGTACACCCTCATTGCCTGCTCTCCGAGGAGGGGACATTATACGGGAACCCGGAACAAAGAGGGGTCCAACACCGCTCAGCCACCCCTGTGGGGCCC
CCCGGGTTCCTCAGAGCCCCCTGGACAGTCCACTCAGAGGGACAGACGAGGAGTTACTGCTGAGGATGAATGAGATTAAACAAGTGAATTTGGTCATTGG
GGCCCCCACACACTGGGCCACATCTGTTATAATGGCGGATATGGTGGTAACCTGTGCTCCTCCCTATTGCTGAGGGGCCCTCGATCAAATGGCGGTTTGG
TGTAACCACGTGGGCTGAAGCCGAGGCTCTATGTCCCTTTACTTCCCTGTCTACTGTGCTTCATAGTGTCCGGAGCTTACACTCATTATTGGTTGCTAAT
AGTCGTTGCTAGGGGTGGTAAAAACCTAATCAAAGTTGGGGTCTGTTGGAATGGGAAATACCAATATGAGGGCAAATGCCTGGAAATTTCACCCAGACCA
GGAAGCAGCGGTTTCGTCGCTGAGTAGTGATGTCATCACTTTTACGTGACATCAGCTTGGGCAGAACCATGGGACAAGTGACGTAGGGGGGGTTTGTCTG
GCCCTAGTGTTGGATCTGGGGCCTGAAGGAAGTAGATTAGCACTTCTGGGGCTTATGCCCACCTACAGAACAGGAAAATATTAGAGGGCCCCTGGGCAAT
ACAGGACATCTGGGACCCCAAACTTAGGCGGGGGGGGGGAGGTCCAATCTGGTGATGGGATACTGAGATCTGTAGTCTGGCAAAGCTGAAGGCCATATCC
TGAAGCATATATGTAGCCAATAGAATATGCTCTTTTTCTCCCATTGGGTCCTGGGCAATATCCTAAACTTGGCCTTGCTGGCCAGTACTGGTAGCTAGTG
GCCAAGGGATGCTCTAGGCATGTATTTGGGTCTGGTAGCCAGTACTGGTAGCTAGTGGTCAAGGGATGCTCTAGGCATGTATTTGGGTTGGCTGGCCAGT
ACTGGTAGCTAGTGGTCAAGGGATGCTCTAGGCTTGTATTTGGGTCGGCTGGCCAGTACTGGGAGCTAGTGGTCAAGGGATGCTCTGAGCATGTATTTGG
GTCGGGTGGCCAGTACTGGGAGCTAGTGGCCAAGGGATGCTCTGAGCATGTATTTGGATCGGCTGGCCAGTACTGGTAGCTAGTGGCCAAGGGATTCTCT
GAGCATGTATTTGGATCGGCTGGTCAGTACTGGTAGCTAGTGGCCAAGGGATGCTCTAGGTGTGTATTTGGGTCTGGTAGCTAGTGGCCAGGGGATGCTC
TAGGCGTGTATTTGGGTCTGGTAGCCAGTATTGGTAGCTAGTGGCCAAGGGATGCTCTGAGCATGTATTTGGATCGGCTGGCCAGTACTGGGAGCTAGTG
GCCACGGGATGCTCTAGGCGTGTATTTGGGTCTGGTAGCCAGTACTGGTAGCTAGTGGCCAAGGGATGCTCTAGGCGTGTATTTGGGTCTGGTAGCCAGT
ACTGGTAGCTAGTGGCCAAGGGATGCTCTGAGCATGTATTTGGATCGGCTGGCCAGTACTGGTAGCTAGTGGCCAAGGGATGCTCTAGGCGTGTATTTGA
GCCTCTGTATTTAGAGGCTATTTAGCCTTGGGCAGGATCTTGGCTTCTGACATAGCCCCCCAGCACTTAGACAGACCTACTCTCTGGCACCCAAGCTACA
CGGCGGCCCTTCACCCCCCCGTTGCCTGTAAGTGCACTCAGTAGCCTAATGAGACTGCGCTCTTGCCATTCAGTTGAATTATTGCGCGCCAGTGGCATTG
GGGGAGAGCATTGCTGGCACTTAGAGATGCCACTTAGTGCACTGGTCTAATTGCTCTGGCAGAGAAAACCTCTCGTTCAGGTTCTGTTTAACAGAGACAG
TTGCTGCCAATGAGACAGCCTCCAGGCAATGGGGGGCGCCCTACAATTCATGGGTATTTTCCATGTAATCCTCGTATTACTAAAAAAAAAAAAAAA
5' EST: CX812223
[+]catggagtcggaggcagtaggggtggctaaggaggtttggggcggctgatttcccagagagaaggatggaagggaattctcccaccccagtgacacagga
atcctgatatccactgtctgaattgggacaatgtactgagacaggctgtcagggaaagagacatacagagagcgtctgggaaagcgttccagggagacgc
ctggctgggcactatgggaaggcagtgcggcgaggagaccatggctgggggcaaaataaggggcacctagggggcaactgggacccactggaacccaaga
gctcacacaaaggcagttagtgggacggcgcggaatcaccagggaattgtgtggattttgtcgccttccaacatcgtgggagatggagtccagcgaggga
taattacaccatcatcctctgtgtgaggcaacaaaatggtggcacgtggcacggacactctggattcagccaatgaaagtcgcctcctttcctccgcagc
caatcccggtgttaaaggaagaccaagcaaaaggatcacgcgaggtcctttcgtaaaattggcagattgtgcccattatcactatgagaatgtcaactac
ggaccaattcagctcggcctgtctgattcccgcactgaggatgccggtagcaccgagggggatgtcatctatattgtacaggtcacatgtcagggacgtt
cctggtccgttctgcgcagttatgaggatttccgggtgttggactccaaccttcaccgttgtatcttcgaccggcgcttctcccaactgctggagctgcc
cccccgctctgagctgcccgcccaaggccagctcctcgccctctgctctctc
3' EST: CX812222
[+]tttttttttttttttagtaatacgaggattacatggaaaatacccatgaattgtagggcgccccccattgcctggaggctgtctcattggcagcaactgt
ctctgttaaacagaacctgaacgagaggttttctctgccagagcaattagaccagtgcactaagtggcatctctaagtgccagcaatgctctcccccaat
gccactggcgcgcaataattcaactgaatggcaagagcgcagtctcattaggctactgagtgcacttacaggcaacgggggggtgaagggccgccgtgta
gcttgggtgccagagagtaggtctgtctaagtgctggggggctatgtcagaagccaagatcctgcccaaggctaaatagcctctaaatacagaggctcaa
atacacgcctagagcatcccttggccactagctaccagtactggccagccgatccaaatacatgctcagagcatcccttggccactagctaccagtactg
gctaccagacccaaatacacgcctagagcatcccttggccactagctaccagtactggctaccagacccaaatacacgcctagagcatcccgtggccact
agctcccagtactggccagccgatccaaatacatgctcagagcatcccttggccactagctaccaatactggctaccagacccaaatacacgcctagagc
atcccctggccactagctaccagacccaaatacacacctagagcatcccttggccactagctaccagtactgaccagccgatccaaatacatgctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: