XB-CLONE-3389910
Clone Name: IMAGE:7652026
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Gene:
psip1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121355 [+]
CGAGAAGCTGGACGTATACACTGTGATCCGGGACTGCGTGCTGTGTTATGAAAATCGGCACTGTCCCGCTTTTGCGGGAGTGTTGAGCAATCTGGGTAGA
ACTTGCTTGAAATTGTTTATCATCTGGTTCCTCTCTGCAAAGTCCCTTCTGCTGCTCGAATCTTGTCAAGAGAAACATGACGCGTGATTTTAAACCTGGA
GATTTGATCTTTGCAAAAATGAAAGGTTACCCACACTGGCCAGCTAGGGTGGATGAACTTCCTGATGGGGCTGTGAAACCTCCTACAAATAAAATGCCAA
TATTCTTTTTTGGCACTCATGAGACAGCTTTTTTGGGACCAAAGGACATTTTCCCTTACGATGAAAATAAGGAGAAGTATGCCAAACCTAATAAGCGAAA
GGGCTTTAACGAGGGGCTTTGGGAAATCGAGAACAACCCTAAGGTTAAATTCTCAACTCCACAGAATATCTTGGCAATCACTAATGCTGCTTTAAGAGAG
GCAGCAGAAGCGCAAATGCAAGAAGATCTAGAGGAAAATGAGGAGAAGGTGACAAAGAGAAGGAAATCAACCACATCAAAGATTTTAGGTAAGCATTCTG
ATTCATCTTCCAGTGGACTTGAGTCTCCAGAGAAAGATATGAACGCCACAAAGGAAGACGCAAGCATCTCTGATCAGCTTGGCAGTGAAGATGCCGGTAT
CATAAAAGATTCACCTGTTGTAGTTAAAGGCAGAAGAGGTAGAAAGAGAAAGGTTGATATACTGGCTGAGTCTGCGCAAGAAATAGCGGCTGCACCAGTA
AGTCCACCAGTACAGAGCCCTAAAAGAGGCAGACCAGCCACAGGAGAAGTTAAGGTTCCAAAGCCAAGAGGAAGGCCAAGATTAATAAAACCCCCTCCCT
CACCAGAACGAGGAATAACAAGTGAAGAAGATCAGAGTAAGAAAAAATCTCCAGAAAGAAAACCAAAAAAAACAGACACTTCAGAAAAAGGATGAGGTCT
TGAAGGATGAAGAAGAAACCCCAAGGAAAGAGACAGACAAAAAGGAGGCAAGAGTGGAGGCCGAGACCAAAAAGAAAGTTGCAGCAAAGACTGATTCTCC
TGCTCAGTCAGATTCTGATGATGATGAGCAAGAAGATGGAAAGAAGGGCAAAGCTGGCAAACTCTCCCAGGTGGCTCAAAAGCGCAGCCTAAAGGCTCAA
CAGGAAAAGGATATTCCTGACAAAAAGCGCAAACAGGAGGAAACTGTTGACATGGAAAGCCAGTCTAAGGATGAGGTGAAGAAAATTGATGTTAAAAAAC
TGGACAAGAAAAAAGAAACATCCACAGATGCCAGACTTCAAAGGATACATGCAGAAATTAAGAGTTCTTTAAAGATTGACCACTTGGATGTAAACAGATG
CATCAGTGCTTTGGATGAGCTGGCTTCTCTCCAAGTAAGCATGCAGCAAGCTCAAAAGCACACAGACATGATAACTACCCTGAAAAAAATTCGCAGGTTC
AAAGCCAGCCAAGTGATTATGGATAAAGCCACCATGCTGTACAACAAATTTAAGAATATGTTCCTAGTTGGAGAAGGCGATTCTGTCATAACTCAAGTCC
TAAATAAATCTCTAGCAGAGCAAAAACAGCATGAGGAAGCCAATAAAGCCAAGGAACCTGGAAAGAGAGGACCTCACAAGAAGGTGGAAAAAGAGCAAAA
TCCAGACACAAATACTTTTAATGGTGACCAGGAATCTCAAGACCAAGCACAACAAAATGGAGACAGCAGTGAGGAGACTGAGGAGAAGGAAGATAACACT
TTAAAGCGCTCTGTAAGTGCATCAAAAGGCAACGAGGCCAGCAAACAAACCAGAGATGATTCTGCCCCGGACAACTAACGCAGCAGACCCCTGCCCTCAG
ACCAGGCTCATGTAGTAGTTTTAAGATTGCACATTTTACACTGCTGAGAATTTTAACTCATTTTATAAAAGCAAACAGTATTTTGAGGTCTTTGGAGATG
AGACATTTTTATGTTTTCCTGAAGTAGTGCTGATCTATATGTCCACTAGCTGTTTGAAGCATTATATGCTGTTTTATTAGCAAAATTGTTGTAATGGACC
TTAAAAAAAGCAAAACCAACGCAGATTTATGCATTTCCCTTCCATTTATTTCAAATTAGTGTGTGTGGATGTGCATGCGCATGCTGCAGTAGGTTGCTTC
TCTTCCGCTCATACAGGACGCCCTGTAAGTCTACCTGGTACAGTGTAACATGCCAAGCCAGTATTTATTTGGAACAAAGTGCACAAAATAAGTGCTTTTG
TACTTACAAAGATGACTAAAACTAAACTAATCCCAATACACCCAGATTATCTCTAGCACATTATCGGTGCGGTAGAGTTTCAGTTTAATGAAACTCTGAT
GGGAGTCACTTTTAAGGATGAGTTATGGCCATCTGTGATAGTCATGAAGGCCATTAACAGGGCATTCTATACAAGTGACATTAGTTTGCTTTACCCATGA
GCTTGCAAGCCACATACTGATATGGTATTAGTTGTCCAGCCACTGTGGTGTTCAACCGTTATAGTGAACATGTGGCAGATAGGCATGAGTCCAGGGATCT
TACATGCCAAGCCCATGTTCTAACCTTCTCAGGTTTTACTTCCTACCCTAATTAGAAATACATCAGGCAACTAGGATCCCATACAGACTGGCCTAGGCAT
TTAAATGTCATGAACTTCTGCCTTTTTCCTTAATAAAGAAGGTGGCCAACATTCATTATGTATATCAAAACCATATGGAAGCCTACCAGGTGGTTGTGCT
TTCCCTTATATTAAATGAGAACATTATTATATATGTATAAATCACCAACATATTCTGCAGGATCAAATATACAATACTTGGGGTCATTTATCAACACTGG
GCAAGTTTACACATGGACGTTAACTCATGGCAACAAATCAAATTGCATTCATTGTTCTACCCGCAGGTGTGTCTGAAAAAGCCACTCACTGATTAATTGC
TACCAGTTACTGCCAATGGGCAAATTTACCCACTGTTGATAAATGAGCCACAGAGAGGTACGAAAGCTCAGTAGAGCTTAGAATATGAAAGGAGAAAGGG
GGTATGAGACAAAAGGGGTGCACTTACTCTTTACAATGTGCCATTCCATATATTTTTCTTTTTTCAAAAGGAAGCCCATAGTGTTGCCAGCGATTATTTT
CTGCATTCAGAAAATACACCATTGTTTGCCTCTGCCTAAAAACTTGCTCTTTACATTCTTTCAGCCAGGTCTGTTTTTCAGTGCAAGTCTAGTATTTCTA
ACATATAAATGTGACTTTACAGCCCTATGGGAATATATGTATATGTTTTAACTGGCTTTATTTTTATCAGCCCTCATTAATGCCTTTGTGGATCTGCAAG
GTACTGTACTGAAGGCTTCTCTGGGCTACACTGATGGTTTGTTGTCTGCTAAAGTCCTAATACTTTACCAGTATTCTTGTTTTCATTGTTGTATTTGTGC
AGATTGTGAAGAAAAGGCAGTATATTTTTTATATAGCAGTGATCAAATTGTGTGTCTTATTTTGCTTGTGTTTTTGTAATTCAGAATCTTGTTTTCTGTT
TTGTATGATGGTCATATGCTGTGCAAAGTAAGAATGCTTTGTAAATAGGCTCCGTGAAACAGATTCGTTGTGCTTTTGTATTGAAGTCTCAAATAAAACT
GTAAAGAGTTCAAAAAAAAAAAAAAA
5' EST: CX814929 [+]cgagaagctggacgtatacactgtgatccgggactgcgtgctgtgttatgaaaatcggcactgtcccgcttttgcgggagtgttgagcaatctgggtaga
acttgcttgaaattgtttatcatctggttcctctctgcaaagtcccttctgctgctcgaatcttgtcaagagaaacatgacgcgtgattttaaacctgga
gatttgatctttgcaaaaatgaaaggttacccacactggccagctagggtggatgaacttcctgatggggctgtgaaacctcctacaaataaaatgccaa
tattcttttttggcactcatgagacagcttttttgggaccaaaggacattttcccttacgatgaaaataaggagaagtatgccaaacctaataagcgaaa
gggctttaacgaggggctttgggaaatcgagaacaaccctaaggttaaattctcaactccacagaatatcttggcaatcactaatgctgctttaagagag
gcagcagaagcgcaaatgcaagaagatctagaggaaaatgaggagaaggtgacaaagagaaggaaatcaaccacatcaaagattttaggtaagcattctg
attcatcttccagtggacttgagtctccagagaaagatatgaacgccacaaaggaagacgcaagcatctctgatcagcttggcagtgaagatgccggtat
cataaaagattcacctgttgtagttaaaggcagaagaggtagaaagagaaaggttgatatactggctgagtctgcgcaagaaatagcggctg
3' EST: CX814928 [+]tttttttttttttttgaactctttacagttttatttgagacttcaatacaaaagcacaacgaatctgtttcacggagcctatttacaaagcattcttact
ttgcacagcatatgaccatcatacaaaacagaaaacaagattctgaattacaaaaacacaagcaaaataagacacacaatttgatcactgctatataaaa
aatatactgccttttcttcacaatctgcacaaatacaacaatgaaaacaagaatactggtaaagtattaggactttagcagacaacaaaccatcagtgta
gcccagagaagccttcagtacagtaccttgcagatccacaaaggcattaatgagggctgataaaaataaagccagttaaaacatatacatatattcccat
agggctgtaaagtcacatttatatgttagaaatactagacttgcactgaaaaacagacctggctgaaagaatgtaaagagcaagtttttaggcagaggca
aacaatggtgtattttctgaatgcagaaaataatcgctggcaacactatgggcttccttttgaaaaaagaaaaatatatggaatggcacattgtaaagag
taagtgcaccccttttgtctcataccccctttctcctttcatattctaagctctactgagctttcgtacctctctgtggctcatttatcaacagtgggta
aatttgcccattggcagtaactggtagcaattaatcagtgagtggctttttcagacacacctgcgggtagaacaatgaatgcaatttgatttgttgccat
gagttaacgtccatgtgtaaacttg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: