XB-CLONE-3384832
Clone Name: IMAGE:7805967
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Gene:
csde1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR842563 [+]
tcgattcaattcggccgaggctttattctatttttcattttttttgccattaaacagaaaaactggagccaagtatcattttataaaggtaatgtgagtc
tccaaaaaaaaaaaaaaacaaaaaaaaaaaaatactttccctctttccgtagaaagtggcttgatcttttttgcttaaaataaaaagggcaacttaattt
aatattttatgcgagcgggtaatggaccgaaacaaataaaatagggctggggggagggtagtttaaaaaaaaaaattatatattaggtaccagctttcta
aatttatcttgatgcgaatgaccaatataactatcaaaagactaaatcttatgataaatcggtgctgtaaacaaaaacaaaaaaaaaaacaatgtttcag
gcacatttttcttccttttaaatccattaatgtttcaaaagctgcagtgtcaaagcagcaaaaacaaaaaaacaaaacaaccgtgcattttcgtattaat
ttaacacacatggcacatttcaggcttgttgtactctgcacttccaaagtcacttgttaacacaaaaaccaaaataaattttgaagaaatgcattcaaaa
actaacagcgtgtgaatgcagagttaattaatcccctctgaaaatgtgaatgtttggctgtcactggaattagaaaatgcttcattttttatcattgctt
agggaacactgcataggatatattccaccatatctatgtggtcctttgatagaaaaataaagcatagaccaatgctgaataaaaaggaattgctatgtta
aatctaaggcaaagtaaaaaaaaaaatgaaaaaaaattcagtaatggaggtagaatatatgctaaacagtatgaaagtgcagaaatctgtacgttaaatc
acattaccaccaaatctgtcatga
3' EST: DR842562 [+]ttggcttcattaactacgaagttggtgacagtaagaaacttttcttccacgtgaaggaggtccaggatggtgttgaactccaagctggggatgaagttga
attctctgtaattctgaatcagcgcacaggaaagtgcagtggttgcaatgttacacttataagcaaaggccctgaaatggtggcagctcctcgccccgac
agattagtaagcaggttaaagagcataacactggatgatgccagtgccccacgtctcatggttttacgtcagcctagaggtcccgataacacaaagggat
ttggtgcagagagaaagatccgtcaagctggggttatcgactgatttccatcttcaaaagcacattacctatttaagtatgatcaaagtaatgcggtctg
tgaggagaaacaacatctttctttatagcccggaaattccacgaatcttatttagcataaggactgcaatcttttaaacatctgccatcttactttcaaa
ccaggaaaccctttttaaattatgcccatttgcaactgaagagttaactgtaagccttatagtatatcatttgtttgttttcatatttttttttttttca
agctttctttcaaatgatgcatcttatcgcaacaattgttttttccttttcatgacagatttggtggtaatgtgatttaacgtacagatttctgcacttt
catactgtttagcatatattctacctccattactgaattttttttcatttttttttttactttgcc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1905 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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