XB-CLONE-3383543
Clone Name: IMAGE:7827751
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Gene:
mvb12a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC155409 [+]
CGGAACTGCGGGCTGGCTTGGGGAAAGCTCTACTCTTGGGTGATTGGGAGTTTTGTGATCAAAATAAGATGGAGAACACTTCTACACCCATCACTGGATT
AGCCTGGATATCTAGCGTCACTCTCTGTCCCAGGACTTACAATATGATTGTGGCAACAGTGGAAGGAGCATCTGCTAACTTTCTTAAGGGGTTTAATCAG
AAATCAGCTGTGTACCTTGGTTACAGCACAGCCCTAGAGGGTCTGGAGCAAGTTATCACTGACATTCAAATACTGAATGAAAAGACTGTGCTGCCTGTTG
GGTATGCTTTCATTGGGGAACACCTAGACCCAAAGATCTCTGTTCCAAAGAAAAAGAGGCTGTGTGTAAAGCAGATGCCTATGCATACTGCAGAAACTGC
TGTCTGTGAGATTAAATTTTCCATGAAAAGCAAGCATATTGGGGCTCCTTTTGAACGTATTGGTGAAATTAGTGGACTGGTATTTTGGTGCAGAAAAGGT
AGTGTACCTTCTCCCAAACCAACGCCAAAACCTCGAAACATTACTAGTGGCATTAAAGGGCTTTCTCTCGATATCAACACCCCAGTCCAGCCAGTAAGAC
GAGCAAGTGCTCCAGTTCCAGATGCAGGGCAAGTTGTTCCACAGAGTAATGGTAAATGGCCATTATCAGAGAATGCTATCTACGATTCTTCAAATATTTA
TGGCATATCTGCTATAGATGGGATTCCTTTTACAATTCACCCAATGTTTGAGAATACGGTTAACAATACTTCGGTGGTCACTTCAGACTTCAGAGATCTA
CATATTAAAACACTGTCTGAGATTGAAAGTGAGTACAATTATGGATTTGTGGTCGAAAGGACAGCAGCGGCTCGTGTTCCCCCACCTTTGCGTTAAGAAG
ACAGAGTTGGCCTTTTGGACTTCATGAGAGAACTGGAAGATTTTGGTATAACTTAATGACAATAATAGAACCTGCGCAGGAAAGAAGATATCTACAGAAA
AAGATTTCTGCAGATTTTGTCAATGATTCCCAAAACACATGGAACAGTACAGCTCTGATAACATGTAATTCCATTAGCTGGACTAATGATGATGACATTT
TGATAATTTAAAAAATTTTAGCCCTCCTTTAAAGGGGTCATTCGCCTTAACGTTTACTATGATGTAGATGGTGATAGTCTAAGACAATTCACAGTTGGTC
TTAAATTTTTACATTTTTTGACATTTTTTTTGTTTTTGAATGAGCTTTTTGTTCAGCATGTCTTCAGTTTTGGATTTTGGGGTCCAGTTTAACCCGGTAA
CCAGGCAGTCATTTGAATGAGAAACTGGAAGATTAGGAGAGGGCCCAAGAGCTATGCAAAAAAAAAATTGTAGCCTCACTGTTCAGTAGCTTTTTGTCTG
CCAGAATCGGCAACCCCTTGCAACTTGATTCAATGAATATGACAACTTATTTGAAAATTAAAATAATAAGAAAATAGTGAAGACCATTTACAAAGTTACT
AACATTCTGTAACATACTAAAATTTGCCGGAAAGGTAGATGTGAATCTGTTGTATCCACTGTGTCTTGTACTACAGCGATTTCCATGAGGGACAGATTTA
TGAACATATTTATTTTAATTTTTAATGTGATTCAAGTTTTTTGTGCTAAAACTCACAAACTCAAATGATCACTTCAGATCAAGTATTTATTAAGCTCAGT
AACTCGGATATTGCAAGGTCATGTAGAAGTCAACATTTAAACTATTTTTTAATTCGAGTTTTTCAAATTTTTCTCAAGGTTGTAAACTTACAAATTCAAG
TGATACTATTTTTTTTTCAGTTGAATGTTTCATATTCTGATTTTTTGAATAAGCAAACATCATTTTTTTTTTTAAATTGTGAGTTTATTCAAAGTAGATC
TCTAAAACCTCACAAGAAGCCATATATTCGTGAAAACTAAAATCTCTTCATGCAGGATGGTCACTAGTCTGTTTTGGTTTTTTTTCTATTTAGTATTTGT
CTTACTAAGAGCATAATAATGTGTAAATATGTGGAGCATAATCTTGTCTCATTTCATGTAATTACAAATTGCTGATTATAATATGAAGAAATGACATACC
TGCCTGAAATGGTACTATATAATTAAATGCCTATTCACATCCTAAAAAAAAAAAAAAAAAA
5' EST: DR843424 [+]ccgaggcggaactgcgggctggcttggggaaagctctactcttgggtgattgggagttttgtgatcaaaataagatggagaacacttctacacccatcac
tggattagcctggatatctagcgtcactctctgtcccaggacttacaatatgattgtggcaacagtggaaggagcatctgctaactttcttaaggggttt
aatcagaaatcagctgtgtaccttggttacagcacagccctagagggtctggagcaagttatcactgacattcaaatactgaatgaaaagactgtgctgc
ctgttgggtatgctttcattggggaacacctagacccaaagatctctgttccaaagaaaaagaggctgtgtgtaaagcagatgcctatgcatactgcaga
aactgctgtctgtgagattaaattttccatgaaaagcaagcatattggggctccttttgaacgtattggtgaaattagtggactggtattttggtgcaga
aaaggtagtgtaccttctcccaaaccaacgccaaaacctcgaaacattactagtggcattaaagggctttctctcgatatcaacaccccagtccagccag
taagacgagcaagtgctccagttccagatgcagggcaagttgttccacagagtaatggtaaatggccattatcagagaatgctatctacgattcttcaaa
tatttatggcatatctgctatagatgggattccttttacaattcacccaatgtttgagaatacggttaacaatacttcggtggtcacttcagacttcaga
gatctacatattaaaacactgtctgagattgaaagtgagtacaattatggatttgtggtcgaaaggacagcagcggctcgtgtt
3' EST: DR843423 [+]aggatgtgaataggcatttaattatatagtaccatttcaggcaggtatgtcatttcttcatattataatcagcaatttgtaattacatgaaatgagacaa
gattatgctccacatatttacacattattatgctcttagtaagacaaatactaaatagaaaaaaaaccaaaacagactagtgaccatcctgcatgaagag
attttagttttcacgaatatatggcttcttgtgaggttttagagatctactttgaataaactcacaatttaaaaaaaaaaatgatgtttgcttattcaaa
aaatcagaatatgaaacattcaactgaaaaaaaaatagtatcacttgaatttgtaagtttacaaccttgagaaaaatttgaaaaactcgaattaaaaaat
agtttaaatgttgacttctacatgaccttgcaatatccgagttactgagcttaataaatacttgatctgaagtgatcatttgagtttgtgagttttagca
caaaaaacttgaatcacattaaaaattaaaataaatatgttcataaatctgtccctcatggaaatcgctgtagtacaagacacagtggatacaacagatt
cacatctacctttccggcaaattttagtatgttacagaatgttagtaactttgtaaatggtcttcactattttcttattattttaattttcaaataagtt
gtcatattcattgaatcaagttgcaaggggttgccgattctggcagacaaaaagctactgaacagtgaggctacaattttttttttgcatagctc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: ovary | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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