XB-CLONE-3382937
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Clone Name: IMAGE:7828092
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Gene:
eif1ad
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121518 
[+]
GTTCCAGCTATTGGAGTACTTTATATCTTTTCGCAGTTTTCTTTATATTAGTTAATTGGGTTATAAAAGCGCTCCGACCTGTGACGCTGTGGTAGTAGTG
CAATTACACATGGCATAGATGCTGAAGAGTCTCAGTTTTACCAGGCTTACATACACAGGCATCTTTTTATGGCTTTGTATCACTGAGATAAAATAATCAC
AACAATGTCAAAAGCCACAAAGCGTAAACATGTGGTTAAGGAGGTTCTTGGTGATTATGTGCAGCCCACTGAGCACCAGCGTATAGTTAAGGTTTTAGGA
AGCCCAGGTAACAATCTCCATGAGGTTGAAACATCTGAAGGGGAACGATTTCTGGCTAGCATGCCAACAAAATTCAGGAAGAACATTTGGATAAAAAGAG
GTGATTTTCTTATTGTTGATCCAATAGCCGAAGGAGAAAAGGTCAAAGCCGAAATAGCTTTCATCCTGTACAAGGATCACCAAAGACTTCTACAAAAGGA
GGGGCTCTGGCCAGAAGGCTTCACGCAAGACAAAACAGGAGTTGTTACCAAGGAGAATGAAAACAATGGAATCCAGAGCACAGAGGCACTGAGCACAGCA
CAAGCAAAGGAACAAGGGGAGGATAGCGAAACAGATGACGACAGCGGCCTGTTTGTTAATACTAATCGTGTACATTATGAAGACAGTGAGGAGGAGTCGG
AAAGCGAAGAAGAGTCAGAGAGTGAAGAAGATGAAGAAAACTGAGTGCAGAAGGCTTAAACTTTGGGGGATAAATGACGCAGTTATCATAATAACTGGAA
AACTACTGGTCTCTAAAACCAGTAAGGACTAATAAAATGTCAAATATTTGTATTTTGTTCTTTTGAGGATGTAAAATTTGCTTGTACTGAATAAAATGTT
TTCTTTATGCAAAAAAAAA
5' EST: DR843825 [+]tccagctattggagtactttatatcttttcgcagttttctttatattagttaattgggttataaaagcgctccgacctgtgacgctgtggtagtagtgca
attacacatggcatagatgctgaagagtctcagttttaccaggcttacatacacaggcatctttttatggctttgtatcactgagataaaataatcacaa
caatgtcaaaagccacaaagcgtaaacatgtggttaaggaggttcttggtgattatgtgcagcccactgagcaccagcgtatagttaaggttttaggaag
cccaggtaacaatctccatgaggttgaaacatctgaaggggaacgatttctggctagcatgccaacaaaattcaggaagaacatttggataaaaagaggt
gattttcttattgttgatccaatagccgaaggagaaaaggtcaaagccgaaatagctttcatcctgtacaaggatcaccaaagacttctacaaaaggagg
ggctctggccagaaggcttcacgcaagacaaaacaggagttgttaccaaggagaatgaaaacaatggaatccagagcacagaggcactgagcacagcaca
agcaaaggaacaaggggaggatagcgaaacagatgacgacagcggcctgtttgttaatactaatcgtgtacattatgaagacagtgaggaggagtcggaa
agcgaagaagagtcagagagtgaagaagatgaagaaaactgagtgcagaaggcttaaactttgggggataaatgacgcagttatcataataactggaaaa
ctactggtctctaaaaccagtaaggactaataaaatgtcaaatatttgtatttttgtcttttgaggatgtaaaatttgcttgtactgaat
3' EST: DR843824 [+]tttttttttgcataaagaaaacattttattcagtacaagcaaattttacatcctcaaaagaacaaaatacaaatatttgacattttattagtccttactg
gttttagagaccagtagttttccagttattatgataactgcgtcatttatcccccaaagtttaagccttctgcactcagttttcttcatcttcttcactc
tctgactcttcttcgctttccgactcctcctcactgtcttcataatgtacacgattagtattaacaaacaggccgctgtcgtcatctgtttcgctatcct
ccccttgttcctttgcttgtgctgtgctcagtgcctctgtgctctggattccattgttttcattctccttggtaacaactcctgttttgtcttgcgtgaa
gccttctggccagagcccctccttttgtagaagtctttggtgatccttgtacaggatgaaagctatttcggctttgaccttttctccttcggctattgga
tcaacaataagaaaatcacctctttttatccaaatgttcttcctgaattttgttggcatgctagccagaaatcgttccccttcagatgtttcaacctcat
ggagattgttacctgggcttcctaaaaccttaactatacgctggtgctcagtgggctgcacataatcaccaagaacctccttaaccacatgtttacgctt
tgtggcttttgacattgttgtgattattttatctcagtgatacaaagccataaaaagatgcctgtgtatgtaagcctggtaaaactgagactcttcagca
tctatgccatgtgtaattgcactactaccacagcgtcacaggtcggagcgcttttataac
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: ovary | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
