XB-CLONE-3379685
Clone Name: IMAGE:7829288
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Gene:
gtf2e1.2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122072 [+]
GAACATTGGCACTGAGTGAAACCCAGCAGGCTTCATGGATAGCAGTTCTTTCTATACCTAAGTACATATTGGATCTGATAAATGAGCATAGTAGGAAGCT
ATTGTATTTTCTGATTATACAGACTGTAGGGGCCACCAGATTATTAACATGGGAGACCAAGAAACCATGACTGAGGTTCCTGCAGCCCTGAAGCGCCTTG
CCAAGTATATGGTCCGTGGCTTTTATGGGCTTGAGTACTCCCTGACCCTCGATGTTCTGATCCGATATCCATGTGTGAAAGAGGACGACATTGGTCTCCT
TCTGAAGTTCGAAAAGAAGCAGCTTCGGACCATCCTTAATACTCTGAAGGCAGACAAGTTCATCAAGTGCCGCATGAGAGTGGAGACCGGCCCAAATGGC
AAGAGCACCAGGCATAATTATTATTACATTAATTACAAAGTTCTGGTAGATGTAGTCAAGTACAAACTGGACCATGTTCGGCGTAAAATAGAATCCGATG
AAAGGGATTCCACCACAAGAGCTTCATTTAAGTGCCCTGGATGCCTCAGCACTTACTCGGACCTGGAGGTCAACCAGCTCTTTGATCCATTCACAGAACT
TTTCCGCTGCACATATTGCAATGTTGAAGTTGAGGAGGATTCTTCCTCCCTGCCCAAAAGAGATGCCAGGACTTTACTAGCCCGGTTCAACGAGCAGATC
GAGCCCATATTTGTGCTGCTTCAAGAAACAGAAGATATTATCCTTCCATGCGAACTGCTTGAACCCCAGCCTACAGAGATCCCAGAGCTGTGCGGAAGCT
TTGACCAACAGTCTAGTTCACTAGCACTGGATCTCCAAGGACAACAAGGAAAGTGGGCCAACAAAAGCTCTGTAGGCAATATGTACGTACAGAACGTGAC
CATAAATGTGCGAGAATCTGACTTTAAGAAAAAAGGCAAAGAGAGGAAGGTAAAGGAGCAGCCGGTGTGGATGAAGGATAGCACTGTGCATGGGAGCCCC
CCAGAGGAATCCAGTGCTAGTTTCAAAACTGAAGCCCCGCTGATTGAGGATGAGAATGCAAACCTGAAGGAAGATAACCCAGATAATGAAGTGATCAGAA
CTCTTCTAATCCATGAGATGAAATCCATGTCTGGGCCAGCTGTCAATTCCTTTCCTAAAAGTGATTCAGGGAGCGATACCAGCGAATCCGACGAAGAGAA
AAAAAGCACAAAACCGGCACCGGGCGAATCGCACAGCAATGCAGAACAGGAGGAGGAGTCGGAGACCGTCGATCCGGTGGTTATGGTCAGTGGGCAGCCT
CATGTCTATAGTGAGGTGAGCCAGAACCCAAGCCTGGTATCTTTCATGACAGAGGAGGAGAGGGAAGCCTATATCACAGTGGGACAGAAAATGTTCCAGT
CCGTTTTTGAATGACACTAAGTGGATTTGGTAAAGCCGTCAAAAGGCTGCTGAACATATTGATTTTTGACTGATTTTTTGAGTATAAAAATATATTATTT
TCATTTTAGCTGGTTTGGTCAGTGTGGCTACACACTGCATCACAAAGCTCACGTTATGTTTGGAATTACATACAAGGGCTCTGCCATTTCTCTGCCAATA
TTTGCCAACCAACAGCTCTGCACTCTGGACTGTGGCCTGAGATAAAATGGCCGACAGATGAACCTTCAGTTTTTAGTTCCTGCTACATTTTGGAAGGTAA
AGAACTAGAGGAACAGTGTTGGTTTTGTGGTGCCTATGTATTGTAAATAAATGTGCACATACAGCATTAACAAAAAAAAAAAAAAAAAA
5' EST: DR846719 [+]gaacattggcactgagtgaaacccagcaggcttcatggatagcagttctttctatacctaagtacatattggatctgataaatgagcatagtaggaagct
attgtattttctgattatacagactgtaggggccaccagattattaacatgggagaccaagaaaccatgactgaggttcctgcagccctgaagcgccttg
ccaagtatatggtccgtggcttttatgggcttgagtactccctgaccctcgatgttctgatccgatatccatgtgtgaaagaggacgacattggtctcct
tctgaagttcgaaaagaagcagcttcggaccatccttaatactctgaaggcagacaagttcatcaagtgccgcatgagagtggagaccggcccaaatggc
aagagcaccaggcataattattattacattaattacaaagttctggtagatgtagtcaagtacaaactggaccatgttcggcgtaaaatagaatccgatg
aaagggattccaccacaagagcttcatttaagtgccctggatgcctcagcacttactcggacctggaggtcaaccagctctttgatccattcacagaact
tttccgctgcacatattgcaatgttgaagttgaggaggattcttcctccctgcccaaaagagatgccaggactttactagcccggttcaacgagcagatc
gagcccatatttgtgctgcttcaaganacagaagatattatccttccatgcgaactgcttgaaccccagcctacagagatcccagagctgtgcggaagct
ttgaccaacagtctagttcactagcactggatctccaaggacacaaggaaagtgggcca
3' EST: DR846718 [+]gttaatgctgtatgtgcacatttatttacaatacataggcaccacaaaaccaacactgttcctctagttctttaccttccaaaatgtagcaggaactaaa
aactgaaggttcatctgtcggccattttatctcaggccacagtccagagtgcagagctgttggttggcaaatattggcagagaaatggcagagcccttgt
atgtaattccaaacataacgtgagctttgtgatgcagtgtgtagccacactgaccaaaccagctaaaatgaaaataatatatttttatactcaaaaaatc
agtcaaaaatcaatatgttcagcagccttttgacggctttaccaaatccacttagtgtcattcaaaaacggactggaacattttctgtcccactgtgata
taggcttccctctcctcctctgtcatgaaagataccaggcttgggttctggctcacctcactatagacatgaggctgcccactgaccataaccaccggat
cgacggtctccgactcctcctcctgttctgcattgctgtgcgattcgcccggtgccggttttgtgctttttttctcttcgtcggattcgctggtatcgct
ccctgaatcacttttaggaaaggaattgacagctggcccagacatggatttcatctcatggattagaagagttctgatcacttcattatctgggttatct
tccttcaggtttgcattctcatcctcaatcagcggggcttcagttttgaaactagcactggattcctctggggggctcccatgcacagtgctatccttca
tccacaccggctgctcctttaccttcctctctttgccttttttcttaaagtcagattctcgcacatttat
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: ovary | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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