XB-CLONE-3377901
Clone Name: IMAGE:7830305
|
Gene:
hmgb3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DR847902 [+]
acttagcgaacaatacagagtgaagatggctaaaggtgaccctaagaagccgaaaggcaagatgtctgcctatgcatattttgtgcagacatgccgtgaa
gaacacaaaaagaaaaatcctgaaatccctgtgaactttgcagagttttccaagaagtgctctgagagatggaagaccatgtcggctaaagagaaatcaa
agtttgacgatctggcaaaggcagacaaagttcggtatgacagagaaatgaaagactttggacctgtaaagaaaggcaagaagaagaaagatccaaatgc
ccctaaaaggccaccatctggctttttcctcttctgctcagaattccgtccaaaaataaaatctacaaaccctggtatttccattggtgatgtggcaaag
aagctaggtgagatgtggaataaccttaacgatggtgagaagcagccatacaacaacaaggcagctaaactcaaggaaaaatatgaaaaggatgttgctg
attacaaatctaaaggaaagtttgactgtgctaaaggagccccaaaattagcacggaaaaaagaggaagacgaggatgatgatgatgaagatgaggagga
ggatgaagaggacgaagaagatgatgatgattaaagttgtacattctgtccatgtgaataccatagagtaggggaaccgaagtcaatgactccatcttan
ataaggtggtcaacttgccctcattaggtttaattgcaaaatttggttatggaatgtttcataatgatgctcttgggattgtaactggtttacattaagt
agcagtgcgtgttaacatgagcaatatgaaactgtcaaagctgtacatatttccaaacattttttaaag
3' EST: DR847901 [+]tttttttttttttttcagaacagtcttaacaatgtaatactgttttatttttgcttcaaaaacatttcagcattctaaacataaaaaacataacaatgca
aatataagtacagaacgtttttgaacttgcagatcctgtactcactcgctcttcacttcagtcagtaaagtagtgtctacagtctttgtttaaaaactac
cgcacttaactaaaaaataaacccataaatgtttctcatgccagctgaaccccttccgacaactaatggcagaatgctaatctcactatatacagaaaga
caactttaagctaaatagacgcccaatgtagtgtaagttgatttcatgtgcatgctttgggctagaaggacaaccccctttgaaaagggtgacctagtcc
agggaagtttgtaaaccctgaggtaccaagagctccaagaaatatgcaccataggaatttgtctagtttacagattacagatatgtacaagtcataaccc
aggattttctaatcaataaccattaagtaacaccttaaataaaaaaatgcttgaaacatttttaaatgctttgttacactaacagcaaagtgcacagagt
gaggagaacactagagcgctttttttctttaaaaaatgtttggaaatatgtacagctttgacagtttcatattgctcatgttaacacgcactgctactta
atgtaaaccagttacaatcccaagagcatcattatgaaacattccataaccaaattttgcaattaaacctaatgagggcaagttgaccaccttatttaag
atggagtcattgacttcggttcccctactctatggtattcacatggacagaatgtacaactttaatcatcatcatcttcttcgtcctcttcatcctcctc
ctcatcttcatcatcatcatcctcgtcttcctct
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: ovary | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: