XB-CLONE-3369689
Clone Name: IMAGE:7833380
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Gene:
LOC100494099
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121611 [+]
CGGCAACTGGCGGCACAGATGAAGCTGCTTCTCTCTCTCCTTTTGGCCCTGACCTTGGCCTCCTGCCAAGGAAACCAAATTAAATGCAAAGACCCCAGTG
AAGAGTATCTGAAGAACAAACTCTTCCGCCATGCACCCGATGCTCAAATACTGCTCTTCAAGCCTGATATCAGCCCAGACCAGGAGTTAAGGACATGCCC
CAAAAGTGTGAACCACAGCTCAACTCTCATCCAAGAACGAAGCATCTCACCATGGTCGTACAGAATCAACGAAAATGTAAACCGCTACCCAAAACAGATA
TTGGAGGCCTACTGCCTATGCAAAGGCTGCATTTCATCTCACAACAAGGGGCAAACCACAGTGGTCAGCGTGCCATTCGATAAAGAGGTGCCCGTCTTGC
ACAAGACCCCCAAGTGTAAGAAAGGGCGCTTTGTCTACAAGCTTCGGTTCATACGGATAGCCCAACTGTGCATCTGTCGGTTTCACTGAGATGGATGGGG
GGGCTGTTTGGGCCTCAGTGTAATTGAAATGCCAGGGTCTGTTTTGAATCCCAGTCCAGATCTGCTCCGGAGCTTCTCTTTTAGACAGCCTCTAAGGAAA
TAGTTTGATCTAAGCAGAAAAGCTACTGTGCTGATTAATAAAATCTTAAGCATTTCTGTACCTAAAACACTGTTTATGCATATAACACCCCAATAGGACG
GACTCAAGAAAGGGTGGGACTTGCTGAGAAATAGGTGGAGCTTGAGAAAGGCCATGGCTGGGTATAATAGTTGTGGTTTTGGGTGTGGCCTAAAAATTCC
CAATATTGTTATAATGATGGCCTGAAATGAAAACGAGAGCAATAACTCTCATCACGCTGCACCAATGCTGGCTGCTCACAATGGAACTGGTTAAACCCAT
ATCTTCATACAATTTGTATAGCTAAAAGGCCAGTCTGTAGCGCTACATGCTTGTACATAAATAGTGTGGAGGTTTCTTTTTTTAATGTAAAATAAATTGT
CCCTGAGTGTTCCACTTCAGTATACACAGGGGAACTGTACCTCCCATAAAGATGTTTATTATATGTAAAATCATTTGTAGGCACTATTGAGAACTTTTAC
ATTTTTGGTCAAGTTGATATATTTTTGTATGTTTTGTAATATATATATATATATCTTTGCTTAAATTTATAAATGACTCATTGTGAATTTGAAAAAAAAA
AAAAAAAAA
5' EST: DR854059 [+]cggcaactggcggcacagatgaagctgcttctctctctccttttggccctgaccttggcctcctgccaaggaaaccaaattaaatgcaaagaccccagtg
aagagtatctgaagaacaaactcttccgccatgcacccgatgctcaaatactgctcttcaagcctgatatcagcccagaccaggagttaaggacatgccc
caaaagtgtgaaccacagctcaactctcatccaagaacgaagcatctcaccatggtcgtacagaatcaacgaaaatgtaaaccgctacccaaaacagata
ttggaggcctactgcctatgcaaaggctgcatttcatctcacaacaaggggcaaaccacagtggtcagcgtgccattcgataaagaggtgcccgtcttgc
acaagacccccaagtgtaagaaagggcgctttgtctacaagcttcggttcatacggatagcccaactgtgcatctgtcggtttcactgagatggatgggg
gggctgtttgggcctcagtgtaattgaaatgccagggtctgttttgaatcccagtccagatctgctccggagcttctcttttagacagcctctaaggaaa
tagtttgatctaagcagaaaagctactgtgctgattaataaaatcttaagcatttctgtacctaaaacactgtttatgcatataacaccccaataggacg
gactcaagaaagggtgggacttgctgagaaataggtggagcttgagaaaggccatggctgggtataatagttgtggttttgggtgtggcctanaaattcc
caatattgttataatgatggcctgaaatgaaacgagagcaataactctcatcacgctgcaccaatgctggctgct
3' EST: DR854058 [+]caaattcacaatgagtcatttataaatttaagcaaagatatatatatatatattacaaaacatacaaaaatatatcaacttgaccaaaaatgtaaaagtt
ctcaatagtgcctacaaatgattttacatataataaacatctttatgggaggtacagttcccctgtgtatactgaagtggaacactcagggacaatttat
tttacattaaaaaaagaaacctccacactatttatgtacaagcatgtagcgctacagactggccttttagctatacaaattgtatgaagatatgggttta
accagttccattgtgagcagccagcattggtgcagcgtgatgagagttattgctctcgttttcatttcaggccatcattataacaatattgggaattttt
aggccacacccaaaaccacaactattatacccagccatggcctttctcaagctccacctatttctcagcaagtcccaccctttcttgagtccgtcctatt
ggggtgttatatgcataaacagtgttttaggtacagaaatgcttaagattttattaatcagcacagtagcttttctgcttagatcaaactatttccttag
aggctgtctaaaagagaagctccggagcagatctggactgggattcaaaacagaccctggcatttcaattacactgaggcccaaacagcccccccatcca
tctcagtgaaaccgacagatgcacagttgggctatccgtatgaaccgaagcttgtagacaaagcgccctttcttacacttgggggtcttgtgcaagacgg
gcacctctttatcgaatggcacgctgaccactgtggtttgccccttgntgtg
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 978 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: stomach | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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