XB-CLONE-3355388
Clone Name: IMAGE:7756513
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Gene:
prdx5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154896 [+]
CTTCGGCTCCCCGTCTCATTCCTCCTCCTGTCACCTCTGCGAGCGGTTGGAAGTTTCGTGAGCAGAACAAGAGCCATGTCTGTAAAGGTTGGAGATCAAC
TTCCTAATGTGACGGTGTATGAGGGGGGGCCTGGGAACAAGGTCAGCATCAGAGATGTGTTTGCTAACAAGAAGGGGGTGCTGTTTGGTGTCCCGGGGGC
GTTCACTCCTGGCTGCTCCAAGACTCATCTGCCGGGGTACGTGGCTCAAGCAGCCGAGCTGAAATCCCGCGGTGCAGCAGTTATTGCCTGCATCTCAGTC
AATGACATCTTTGTGATGAGCGAGTGGGCAAAGGCATATGATGCAGAAGGGAAGGTTTGCATGCTGGCAGATCCCTGTGGAGATTTTGCCAAGGCATGTG
GGCTTCTTCTGGACAAAAAGGAGTTATCAGAATTATTTGGAAACCAGCGTTGCAAAAGATTTTCTATGGTAGTTGAAGATGGAAAAGTTAAAGCCATTAA
TGTAGAGGAAGATGGGACTGGCCTGACCTGCAGCCTCGCAGGGAACATCATGTCTCAGCTTTAAGAGACAACTGCATTGTCATCAAATAAATGTGACTGC
TCTCTGTTAGAGGAAAACACTATGAACACTCTTTGCCTATGTTACAGAAATATGAAATAAATAATAGATATATGAACAAAAAAAAAAAAAAAAAA
5' EST: DR873379 [+]catcgattcgcttcggctccccgtctcattcctcctcctgtcacctctgcgagcggttggaagtttcgtgagcagaacaagagccatgtctgtaaaggtt
ggagatcaacttcctaatgtgacggtgtatgagggggggcctgggaacaaggtcagcatcagagatgtgtttgctaacaagaagggggtgctgtttggtg
tcccgggggcgttcactcctggctgctccaagactcatctgccggggtacgtggctcaagcagccgagctgaaatcccgcggtgcagcagttattgcctg
catctcagtcaatgacatctttgtgatgagcgagtgggcaaaggcatatgatgcagaagggaaggtttgcatgctggcagatccctgtggagattttgcc
aaggcatgtgggcttcttctggacaaaaaggagttatcagaattatttggaaaccagcgttgcaaaagattttctatggtagttgaagatggaaaagtta
aagccattaatgtagaggaagatgggactggcctgacctgcagcctcgcagggaacatcatgtctcagctttaagagacaactgcattgtcatcaaataa
atgtgactgctctctgttagaggaaaacactatgaacactctttgcctatgttacagaaatatgaaataaataatagatatatgaacaaaaaaaaaaaaa
aaaaa
3' EST: DR873378 [+]gttcatatatctattatttatttcatatttctgtaacataggcaaagagtgttcatagtgttttcctctaacagagagcagtcacatttatttgatgaca
atgcagttgtctcttaaagctgagacatgatgttccctgcgaggctgcaggtcaggccagtcccatcttcctctacattaatggctttaacttttccatc
ttcaactaccatagaaaatcttttgcaacgctggtttccaaataattctgataactcctttttgtccagaagaagcccacatgccttggcaaaatctcca
cagggatctgccagcatgcaaaccttcccttctgcatcatatgcctttgcccactcgctcatcacaaagatgtcattgactgagatgcaggcaataactg
ctgcaccgcgggatttcagctcggctgcttgagccacgtaccccggcagatgagtcttggagcagccaggagtgaacgcccccgggacaccaaacagcac
ccccttcttgttagcaaacacatctctgatgctgaccttgttcccaggccccccctcatacaccgtcacattaggaagttgatctccaacctttacagac
atggctcttgttctgctcacgaaacttccaaccgctcgcagaggtgacaggaggaggaatgagacggggagccgaag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: