XB-CLONE-3355303
Clone Name: IMAGE:7756569
|
Gene:
rps6
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DR873435 [+]
ccactgggctgccaaaagctcatcgaagtggaggatgagcgcaagctgcgtaccttctatgagaagcgcatggctacagaggttgctgcagatcccttgg
gtgatgagtggaagggatatgtcgttcgcatcagcggtggaaatgataagcaaggctttcccatgaaacagggagtgctaactcatggccgtgttcgtct
tctgttgagcaagggtcattcctgttatcgccccaggaggactggtgaacgcaagcgcaagtctgttcgtgggtgtattgtggatgctaacctgagtgtc
ctgaacttggttattgttaggaaaggcgagaaggatattcctggacttacagacaacactgttcctcgtcgcctgggtcccaaaagagccagcagaatcc
gcaaactgttcaacttgtcaaaagaagatgatgtgcgtcaatatgtagtgaggaagcctctggctaaggaggggaagaagcccaggaccaaggcccctaa
aatccagcgtctagtgaccccgagagttctgcagcacaagcgcagacgtattgctttgaagaagcagcgcactcagaagaataaggaagaggcatcagag
tatgctaaacttctggctaagagaacaaaggaagccaaggaaaaacgccaggagcaaattgccaagaggcgcagactgtcttctttgagagcctccacat
ccaaatctgaatcgagtcagaaataaaactccatcatgtaaaaataaatacattttgttgtaaaaaaaaaaaaaaaaaa
3' EST: DR873434 [+]acacaaaatgtatttatttttacatgatggagttttatttctgactcgattcagatttggatgtggaggctctcaaagaagacagtctgcgcctcttggc
aatttgctcctggcgtttttccttggcttcctttgttctcttagccagaagtttagcatactctgatgcctcttccttattcttctgagtgcgctgcttc
ttcaaagcaatacgtctgcgcttgtgctgcagaactctcggggtcactagacgctggattttaggggccttggtcctgggcttcttcccctccttagcca
gaggcttcctcactacatattgacgcacatcatcttcttttgacaagttgaacagtttgcggattctgctggctcttttgggacccaggcgacgaggaac
agtgttgtctgtaagtccaggaatatccttctcgcctttcctaacaataaccaagttcaggacactcaggttagcatccacaatacacccacgaacagac
ttgcgcttgcgttcaccagtcctcctggggcgataacaggaatgacccttgctcaacagaagacgaacacggccatgagttagcactccctgtttcatgg
gaaagccttgcttatcatttccaccgctgatgcgaacgacatatcccttccactcatcacccaagggatctgcagcaacctctgtagccatgcgcttctc
atagaaggtacgcagcttgcgctcatcctccacttcgatgagcttttggcagccagtgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: