XB-CLONE-3355267
Clone Name: IMAGE:7756381
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Gene:
hsp30d
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR873459 [+]
aatctctccagctcttctctgacaagaagctccaggaaatcagcagaagatgtttcctgtcagcctcctacagccctcacactcccctctgtgtccctgc
agccagcctgcactcacactctggccagccactcgcctcatccttggccagctgggagatgacattctgagcatgaggaatgacatggagaggagaatgc
agtgtgtgaatgaggcttatcggctcctctctcaggacatggatatgagaagaataacagaccagagccgacagcccagagccgcagaaactgaggggac
ctctcccagctcaggcaaagatgggaaggatcactttgagctgacgctggatgtgagggacttttctcctcatgaactaacagtgaaaatgcagggaagg
agagtgattgtgacaggaaaacatgagaggaaaagtgacagtgaggatgggagctacgtccatgaatacagacagtggaagagagaagctgaactgccag
aaggtgtgaatcctgagcaagttgtgtgctccttatccaaggacgggcacctacatattcaggctcctcggctggcactgccacctgccccagagagacc
cattcctatcagcatggatccggcccccagagatgcacaggaaatcccacccgatgcccagaacagcaatgcagatggcgagcaacagatggactgaaat
ctccctgataacataaacctttatatgtctaaatattgatattattattatattttaatatgatttttagcaaatcaaaatgcatatattttatagtgac
aaaatatagtct
3' EST: DR873458 [+]ggaggcttttaatttgctttattaaaaaaaaaagactatattttgtcactataaaatatatgcattttgatttgctaaaaatcatattaaaatataataa
taatatcaatatttagacatataaaggtttatgttatcagggagatttcagtccatctgttgctcgccatctgcattgctgttctgggcatcgggtggga
tttcctgtgcatctctgggggccggatccatgctgataggaatgggtctctctggggcaggtggcagtgccagccgaggagcctgaatatgtaggtgccc
gtccttggataaggagcacacaacttgctcaggattcacaccttctggcagttcagcttctctcttccactgtctgtattcatggacgtagctcccatcc
tcactgtcacttttcctctcatgttttcctgtcacaatcactctccttccctgcattttcactgttagttcatgaggagaaaagtccctcacatccagcg
tcagctcaaagtgatccttcccatctttgcctgagctgggagaggtcccctcagtttctgcggctctgggctgtcggctctggtctgttattcttctcat
atccatgtcctgagagaggagccgataagcctcattcacacactgcattctcctctccatgtcattcctcatgctcagaatgtcatctcccagctggcca
aggatgaggcgagtggctggccagagtgtgagtgcaggctggctgcagggacacagaggggagtgtgagggctgtaggaggctgacaggaaacatcttct
gctgatttcctggagcttcttgtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: