XB-CLONE-3355168
Clone Name: IMAGE:7756485
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR873524 [+]
ctttgctgtaactccgacgctgcccgagctctgagggcaaaggagaaccaagccatggccttaccgacccagcaggctgatgagttgcgcctctaccagc
aaactctgctccaagatggactcaaggacatgctggaccacgacaactttatagactgtgttctaaagatccagggcaaggagtttccctgccaccgcct
ggtgctggccgcctgtagcccttacttccgagccatgttcctttccaacctggaggagggcaagaagaaggaaattgacctagaagatgtcgatccagac
gtgatggggaagatcctccattatatctacacttctgagatcgagatcacagagaagaacgtccaggacatattctcggtggccaacatgttccagatcc
cctcgatattcacagtgtgtgtgtctttcctccagaagaagctctgccttagcaactgtctggccatattccgcctgggcttgctgttggattgtccccg
tctggccgtctccgccagggactttgtatgcgaccgattccacctgattgccagagacgaagagttctacgacctttcccccgatgagctgattgccgtt
atctccagcgactccctcaacattgagaaggaagaagaagtctttgaggtggtcctgaaatgggcgttgaaagagaaagaaaaacgcgccaaggcgttgc
ccatcatctttgagagcattcgcttccgccttataccccaagactatataaagaacaaggtggagaagcacgagttggtcaagtccgaacaggaactctt
gaaaaaactccagatggtcaaagacgcccaa
3' EST: DR873523 [+]gaaagcagatttttgtatctttattttcctttatcatgtaaaaatctcagtagaattagcactgattttgtacgttgagtttgaagattcaaatataaaa
atccttgtgcaaccataaatatattgtttcctgtacaaacagaggcctatggggataccggtgttgccccgacagccaattaaaagcattagcggctgac
ctttattgcaggaagtccgtgctggctgggaattctgggagttttagtatagctatagataaggaaccgaagatccttactttgataggtatcggggaat
ttaggtacctgccctggtccagtaacccatggcaaccaattgctcattttcattgctggaattgtaccagaccagtttactggactgcaggcaatttttt
gaaaatcatttagcaactgggtgtgattcagaccagtctaggagagctccgattggggttgtaaggattttttgggggtaaaatgctgcctcaggtgagg
aatggttgcatatgttgagatggggctttggtttcctacaaatagggatgcaccaaatccattattttggggtttgaccgaacaacaaattctttgtgaa
agattcatccgaatactgaactgaacccaaatcggccagacacttggatttggccaaatcctgctgaacctgatcctggattcagtgcatccctaccaat
gatataatctgtgggtatatgtaccataatatcagcttgaactgcctctgtggctaggatggctactgttgggcattgtggccgcacacgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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