XB-CLONE-3354532
Clone Name: IMAGE:7756735
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Gene:
arrdc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR873945 [+]
ggatccatcgattcgctcgagttttttttttttttctagacaattgtacattctagctgaaattgtcaaatgcctgacatcacccagcatgttgcaggct
ttgcttgcccagagcacagaaaccaaactgttaagggctaagcatttcacacaaccaaagttaatgtttacagtacagtcagtctggttcatagtttgct
tagcaggtgctgagctaaagaatgttctgcctttttcattctttaaacattgtagagcagatattttccccctgtaatttctacagtgcctgcatacaac
caaagattgaactgggatctaaaaaagaaaaaagtttacttttattatgacatctgcttgacctatggaatagctatacacacagtatctgccttaattt
gttattccatgccatggcagcagtagccttaaagcaaacctttttttttttttttttaagtcctcttaagggagaaaaaaaaaagatacatattaattat
gagttgaaagtgaactttataaaattgtaatagtgtaagctttgtaagtttatatttcaagcacattgatttattatttattcaatctgtaaaatcacct
tatattttttcagtattaatgaaagaggattgctttgaaagcggaatttggtgctacagtgccttttcattctattaagtgaccttacatataagtcagg
actttaaactctgagatgggattactcatgaccctcgggctgttgaaaaaatacttcagtgggaaatttccagatattccctgtattccctgtaaaacaa
aaaaaaaa
3' EST: DR873944 [+]gttttacagggaatacagggaatatctggaaatttcccactgaagtattttttcaacagcccgagggtcatgagtaatcccatctcagagtttaaagtcc
tgacttatatgtaaggtcacttaatagaatgaaaaggcactgtagcaccaaattccgctttcaaagcaatcctctttcattaatactgaaaaaatataag
gtgattttacagattgaataaataataaatcaatgtgcttgaaatataaacttacaaagcttacactattacaattttataaagttcactttcaactcat
aattaatatgtatcttttttttttctcccttaagaggacttaaaaaaaaaaaaaaaaaggtttgctttaaggctactgctgccatggcatggaataacaa
attaaggcagatactgtgtgtatagctattccataggtcaagcagatgtcataataaaagtaaacttttttcttttttagatcccagttcaatctttggt
tgtatgcaggcactgtagaaattacagggggaaaatatctgctctacaatgtttaaagaatgaaaaaggcagaacattctttagctcagcacctgctaag
caaactatgaaccagactgactgtactgtaaacattaactttggttgtgtgaaatgcttagcccttaacagtttggtttctgtgctctgggcaagcaaag
cctgcaacatgctgggtgatgtcaggcatttgacaatttcagctagaatgtacaattgtctag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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