XB-CLONE-3354464
Clone Name: IMAGE:7756693
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Gene:
hsp30c
,
hsp30d
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR873990 [+]
cttctctgacaagaagctccaggaaatcagcagaagatgtttcctgtcagcctcctacagccctcacactcccctctgtgtccctgcagccagcctgcac
tcacactctggccagccactcgcctcatccttggccagctgggagatgacattctgagcatgaggaatgacatggagaggagaatgcagtgtgtgaatga
ggcttatcggctcctctctcaggacatggatatgagaagaataacagaccagagccgacagcccagagccgcagaaactgaggggacctctcccagctca
ggcaaagatgggaaggatcactttgagctgacgctggatgtgagggacttttctcctcatgaactaacagtgaaaatgcagggaaggagagtgattgtga
caggaaaacatgagaggaaaagtgacagtgaggatgggagctacgtccatgaatacagacagtggaagagagaagctgaactgccagaaggtgtgaatcc
tgagcaagttgtgtgctccttatccaaggacgggcacctacatattcaggctcctcggctggcactgccacctgccccagagagacccattcctatcagc
atggatccggcccccagagatgcacaggaaatcccacccgatgcccagaacagcaatgcagatggcgagcaacagatggactgaaatctccctgataaca
taaacctttatatgtctaaatattgatattattattatattttaatatgatttttagcaaatcaaaatgcatatattttatagtgaccaaatatagtctt
ttttttttaataagcaaattaaaatatg
3' EST: DR873989 [+]catattttaatttgctttattaaaaaaaaaagactatattttgtcactataaaatatatgcattttgatttgctaaaaatcatattaaaatataataata
atatcaatatttagacatataaaggtttatgttatcagggagatttcagtccatctgttgctcgccatctgcattgctgttctgggcatcgggtgggatt
tcctgtgcatctctgggggccggatccatgctgataggaatgggtctctctggggcaggtggcagtgccagccgaggagcctgaatatgtaggtgcccgt
ccttggataaggagcacacaacttgctcaggattcacaccttctggcagttcagcttctctcttccactgtctgtattcatggacgtagctcccatcctc
actgtcacttttcctctcatgttttcctgtcacaatcactctccttccctgcattttcactgttagttcatgaggagaaaagtccctcacatccagcgtc
agctcaaagtgatccttcccatctttgcctgagctgggagaggtcccctcagtttctgcggctctgggctgtcggctctggtctgttattcttctcatat
ccatgtcctgagagaggagccgataagcctcattcacacactgcattctcctctccatgtcattcctcatgctcagaatgtcatctcccagctggccaag
gatgaggcgagtggctggccagagtgtgagtgcaggctggctgcagggacac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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