XB-CLONE-3354318
Clone Name: IMAGE:7756993
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR874086 [+]
ttttttgttcacaagtttaattgtaaaaccaggaaagtttaaaaaaaaaaaaccatataagaacatagcgacacaagaatgatcagggctaatacacaac
acacatttacttctcagtgatgctgtgactgggtgcactgttctacaaggtttcatttaatacaccctgacactatctagcaccccgagcagaagcctag
tgtgatgccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatcatagaactaac
tggaacacagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattcacttattgaata
gaaggctacaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagttggagcctata
aaggttgctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaagcaggtgatcc
ctcaccaggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccaggactgaaccatatcaccctgatgatggggagtag
gtgatatgactgtgtttaggtccccagcccagtaaagtcacatgacgaanagaggtgttctgatttggtccagccaaagcctaaggacctgtaagggttt
ggattttagaa
3' EST: DR874085 [+]gcgagaggcgccacagggtgccccagttaagaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcccaccaggtccca
atcaaatccaaaacataccaggttatgcaggagtgtgaacaatctggaacaacagccccttctgtgttcagccagaacaggacaggcggagaaacagcct
ttgacaagccaaaggaaggcccagcgaaaagtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatttttgttcccacc
ttctactcattgtgctgcgcacatcatgcagagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagttactgtaagacttt
atttcattgctcctatggtcgggctgtgctgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctcttcagttattaagt
caacaaaggagccaaatatgattttttagatttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaacccttacaggtc
cttaggctttggctggaccaaatcagaacacctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcacctactccccatcatc
agggtgatatggttcagtcctggcatttttcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgagggatcacctgcttta
attgttgcattanatgtaaaattaggactttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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