XB-CLONE-3354315
Clone Name: IMAGE:7757041
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR874088 [+]
agaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggtatcaccattgacatctc
cctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgatcactggtacctctcaggct
gactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgccctccttgcctacactc
tgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagccagaaaagatatgaggaaatcgttaaggaagtcagcac
atacatcaagaagattggttacaaccctgatactgttgcctttgtacctatttctggatggaacggtgacaacatgcttgagcccagcgccaatatgcct
tggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactaccctgctggaagctcttgactgcattttgccaccaagtcgcccaactg
ataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggtactgtaccagttggtcgtgtggagactggtgtccttaagccaggcat
ggtggttacttttgcccctgttaatgtaacaactgaagttaaatctgttgaaatgcaccatgaagcccttagcgaggccatgcccggtgacaatgt
3' EST: DR874087 [+]gaaggctttaactttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaacactaataacttaaa
aactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatg
gccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctga
gcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgac
caagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtc
ttccagtttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatcca
gcaccaatctggcctgggtggttcaggatgataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccac
gacggacgtccttcacagaaacgtttttcacgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaac
ttcagttgttacattaacaggggcaaaagtaaccaccatgcctgggcttaaggacaccagtctccacacgac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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