XB-CLONE-3353997
Clone Name: IMAGE:7756880
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR874298 [+]
cttctgtgttcagccagaacaggacaggcggagaaacagctctttgacaagccaaaggaaggcccagcgaaaagtgtctttggttaatggcaaaagaaca
ttcaaataactcggctgcccagcaagtcatttttgttcccaccttctactcattgtgctgcgcacatcatgcagagtcttacactatatccgcgccagtc
atttcactgtctgttatctgtttctaagttactgtaagactttatttcattgctcctatggtcgggctgtgctgcattatctgatatactttcagtagag
acccatttatatgttttctaaactggctcttcagttattaagtcaacaaaggagccaaatatgattttttagatttcctatatataaaatggaggaagtg
aatacagaatttgtattttctaaaatccaaacccttacaggtccttaggctttggctggaccaaatcagaacacctcttttcgtcatgtgactttactgg
gctggggacctaaacacagtcatatcacctactccccatcatcagggtgatatggttcagtcctggcatttttcctatgtcaacatattaaggtggattc
aggactagaccatttcctgcctggtgagggatcacctgctttaattgttgcattaaatgtaaaattaggacttttcccgttaggtactcatccctaacaa
actaagggctgataatctgagcaacctttataggctccaactgtagaataaaaacggcgtccatatctncagtagccagtaatgatattagaagacagaa
taaagagaaagttgaccttgtagccttctattcaat
3' EST: DR874297 [+]gttcacaagtttaattgtaaaaccaggaaagtttaaaaaaaacaaaccatataagaacatagcgacacaagaatgatcagggctaatacacaacacacat
ttacttctcagtgatgctgtgactgggtgcactgttctacaaggtttcatttaatacaccctgacactatctagcaccccgagcagaagcctagtgtgat
gccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatcatagaactaactggaac
acagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattcacttattgaatagaaggc
tacaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagttggagcctataaaggtt
gctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaagcaggtgatccctcacc
aggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccaggactgaaccatatcaccctgatgatggggagtaggtgata
tgactgtgtttaggtccccagcccagtaaagtcacatgacgaanagaggtgttctgatttggtccagccaaagcctaaggacctgtaagggtttgga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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