XB-CLONE-3353897
Clone Name: IMAGE:7756746
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Gene:
wwc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR874364 [+]
caggataacaaaacctttaacatttgctgactgtgttgggaatgagctacctttgggatgggaaattgttcgcgatcaacagataggcgtatactacatg
gaccacattaaccaacttacacaggttgaggacccacgagagcagtggagacgagaacaggaacgtatgctgaaagaatacttggtagtagcccaggagg
ctcttaatgccaagaaggagatttaccaaattaagcagcagcgttttgaattggcacaagaagagtttcaacagttgcatgatctctgtgaagatgacag
tcgttcttatgtcagctccttttctggattctcagcaaacacaaagtatgatccgtaccaaattaaggctgaaattgcaaaccggcgagacaggctttcc
aggttaaaacgagagctgacagtgatgaagcaagaactgcaatacaaagaaaaaggagtggaaaccttacaagagattgaccgcaaaatgtccagtgctc
acaccaactataaacttgatgaagcacaagcaataatgagtgaattaagaacattacgccaagcaatttgcatgggagaaaaggaaaggcgtgaccttat
gcagagcctagcaaagctgactgatggattcagaaacagctgttgtatttcagactctctatacggcgctcagcacaattcaggctccctaactgactcc
tgtttacctcagcaatactgtgatgcctgctcgcaaactgataacatcggagagtttggctttgatgataaatccagattggcagatagagtcaggctta
actggcagtatgaggaagccaaaaaaagagtttccagcatacagcatcagctggccctcttgataat
3' EST: DR874363 [+]gaaaaatgcaatgccattttaatcagttttacaaatctggatttgataatattgcagtacataagagagttggtgcttagcaataagatttacatacaca
ttaaagagaagcggcaaaaaagctactaaaaccaactgcaaaacagtatttcagttcaggtaccagaaacaaagccagctggacaaggtgaattcatctg
tataaagaacaccggaaagcagaaactcagtctaaagacatcttaaggcaggtattttccttcaaggaaaacagagcactttaaataaattacactccag
ggaattcccctgaactggctttgtttctgatttccagcctttattaattcacataaaacagtgtcagcactgtacacagacaagacagttgatctataaa
ataggttgttcttcaaatgccaatgttacaacaaggaaaaaccaagccttgctgcagtcattttataaaatatataattatagatatttatatatgcata
tatgtttttgtttttattttttacaaaaaatagcttttgaaacatttatgagataaaagtaacttagtctaataacttcatggagataacacaaacatca
atactcaatgtttctaatgaaacatgtcatacatcgtcagcgggcaaaggaggtatgttgattcttggtcttgtgaagaatgctatcttctccctgaagg
tctgcacttgaaatggttccttctgattctgcccacgcagatggaatatctctttggatgccttcttaagcattttctctgcagcttgttcctgatgatg
ttcaagtttggtctgtctcgtctgtctttctgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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