XB-CLONE-3353432
Clone Name: IMAGE:7757219
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR874673 [+]
ctctttgctgtaactccgacgctgcccgagctctgagggcaaaggagaaccaagccatggccttaccgacccagcaggctgatgagttgcgcctctacca
gcaaactctgctccaagatggactcaaggacatgctggaccacgacaactttatagactgtgttctaaagatccagggcaaggagtttccctgccaccgc
ctggtgctggccgcctgtagcccttacttccgagccatgttcctttccaacctggaggagggcaagaagaaggaaattgacctagaagatgtcgatccag
acgtgatggggaagatcctccattatatctacacttctgagatcgagatcacagagaagaacgtccaggacatattctcggtggccaacatgttccagat
cccctcgatattcacagtgtgtgtgtctttcctccagaagaagctctgccttagcaactgtctggccatattccgcctgggcttgctgttggattgtccc
cgtctggccgtctccgccagggactttgtatgcgaccgattccacctgattgccagagacgaagagttctacgacctttcccccgatgagctgattgccg
ttatctccagcgactccctcaacattgagaaggaagaagaagtctttgaggtggtcctgaaatgggcgttgaaagagaaagaaaaacgcgccaaggcgtt
gcccatcatctttgagagcattcgcttccgccttataccccaagactatataaagaacaaggtggagaagcacgagttggtcaagtccgacaaggaactc
ttgaagaaactccagatggtcaaaga
3' EST: DR874672 [+]gagcagatttttgtatctttattttcctttatcatgtaaaaatctcagtagaattagcactgattttgtacgttgagtttgaagattcaaatataaaaat
ccttgtgcaaccataaatatattgtttcctgtacaaacagaggcctatggggataccggtgttgccccgacagccaattaaaagcattagcggctgacct
ttattgcaggaagtccgtgctggctgggaattctgggagttttagtatagctatagataaggaaccgaagatccttactttgataggtatcggggaattt
aggtacctgccctggtccagtaacccatggcaaccaattgctcattttcattgctggaattgtaccagaccagtttactggactgcaggcaattttttga
aaatcatttagcaactgggtgtgattcagaccagtctaggagagctccgattggggttgtaaggattttttgggggtaaaatgctgcctcaggtgaggaa
tggttgcatatgttgagatggggctttggtttcctacaaatagggatgcaccaaatccattattttggggtttgaccgaacaacaaattctttgtgaaag
attcatccgaatactgaactgaacccaaatcggccagacacttggatttggccaaatcctgctgaacctgatcctggattcagtgcatccctaccaatga
tataatctgtgggtatatgtaccataatatcagcttgaactgcctctgtggctaggatggctactgttgggcattgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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