XB-CLONE-3352581
Clone Name: IMAGE:7757392
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Gene:
klhl21
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR875236 [+]
atcaatattttaatggcttcattttaaagtatacaatggggaaaatacaataaacaaaatccaaaaagtcaaatgttctacaaaaacacacaaacaaaac
agctttagtaagaaattccacaaaccccacagtgctacagtgacgtgtgcaactacttcattaaggggcacagtctttgtagggattggaacccttttac
tcactcccaattctttttttttcttcttctcaccctctgctaaattctcccagttgtaccctgggaaaaatatgaatttagtgaagaaacggcaaacagg
atcctgaataaagcagatggaaatgtgcctggtggtcaggttactgcagtgctaactggaggaagtgcagctcttatgtcagtagataaggtaatggtat
aaagcaggtatgtgcagcatgtgtctctgatgctgttatttaacaagcatttgtttgtctgcaaattccaggttatagtttcggttaataattagaaacc
aggtcaccaattgggactgagccaccagtgcttaagccagaagacattatgggcctatttctaaacaatcaatgttaagttaaagatgaatggtgacctg
tcaccatgtagggtcttgctgggctatcctttgagtgtcagtgacactgcacatgctcagtgtgctccaaacagctgttaaaaagctaagcttaggggcc
agtagtaaaataaaggttacatctgtgttagactgtaccagact
3' EST: DR875235 [+]tggctcacaggctgatcagatgtttactgtacgggattggcccattgaggattgaacctcagactcctgatgtgtctgcatctggctgcactgccacctt
tgcaagtctggtacagtctaacacagatgtaacctttattttactactggcccctaagcttagctttttaacagctgtttggagcacactgagcatgtgc
agtgtcactgacactcaaaggatagcccagcaagaccctacatggtgacaggtcaccattcatctttaacttaacattgattgtttagaaataggcccat
aatgtcttctggcttaagcactggtggctcagtcccaattggtgacctggtttctaattattaaccgaaactataacctggaatttgcagacaaacaaat
gcttgttaaataacagcatcagagacacatgctgcacatacctgctttataccattaccttatctactgacataagagctgcacttcctccagttagcac
tgcagtaacctgaccaccaggcacatttccatctgctttattcaggatcctgtttgccgtttcttcactaaattcatatttttcccagggtacaactggg
agaatttagcagagggtgagaagaagaaaaaaaaagaattgggagtgagtaaaagggttccaatccctacaaagactgtgccccttaatgaagtagttgc
acacgtcactgtagcactgtggggtttgtggaatttcttactaaagctgttttgtttgtgtgtttttgtagaacatttgactttttggattttgtttatt
gtattttcccattgtatactttaaaatgaagccattaaaatattgat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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