XB-CLONE-3352362
Clone Name: IMAGE:7757747
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Gene:
psmb7
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR875380 [+]
catcgattcgtccgctcggaggttttgatttcagcaactgcaggagaaataccctcttgaccaatgaatttactcagaaagggcacaagctacccgcagc
tcgcaaaacagggaccacaattgctggaatagtgtttaaggatggagttatacttggagctgacacaagagcaacagaaggaatgattgttgctgataag
aactgctcaaagatccactatatagctccaaatgtctattgctgtggtgctgggactgcagctgatacagaaatgacaacgcagatgatctcttccaaca
tggagctacattctctgtccacgggacgtttatccagggtagcaactgccaaccgaatgctgaagcagatgctatttaggtatcaaggttatattggtgc
tgcgctggtactgggtggagtggactgtactgggccccatctgtacagcatttaccctcatggatccactgacaagcttccctacgttactatgggctct
ggttcgctggctgccatggctgtttttgaggatcgctacaaacctgacatggaggaagaagaagccaagcagctggtacgcgatgccatcgccgctggga
tctttaatgacctgggctccggcagcaacattgatctatgcgtaataacaaaaaataaggtggattatatccgtccccacgagttggccaacaagaaagg
cgtgaggacaggaaattataaatacaagaaaggtaccacggggattctgtcggagaagattaccaacttcaacttggatgtgatggaagaatccgtgcaa
actatggatatttcctaagtctcttaagtaaatgtattggcatctttttgcatccccgtgtagcccacattatcatgttaagcattttctgcgtttatct
atgatccattaaaaaaa
3' EST: DR875379 [+]gcgagaggctttttttttaagttttgttttttttaatggatcataagataacgcagaaaatgcttaacatgataatgtgggctaacacgggatgcaaaaa
gatgccaatacatttacttaagagacttaggaaatatccatagtttgcacggattcttccatcacatccaagttgaagttggtaatcttctccgacagaa
tccccgtggtacctttcttgtatttataatttcctgtcctcacgcctttcttgttggccaactcgtggggacggatataatccaccttattttttgttat
tacgcatagatcaatgttgctgccggagcccaggtcattaaagatcccagcggcgatggcatcgcgtaccagctgcttggcttcttcttcctccatgtca
ggtttgtagcgatcctcaaaaacagccatggcagccagcgaaccagagcccatagtaacgtagggaagcttgtcagtggatccatgagggtaaatgctgt
acagatggggcccagtacagtccactccacccagtaccagcgcagcaccaatataaccttgatacctaaatagcatctgcttcagcattcggttggcagt
tgctaccctggataaacgtcccgtggacagagaatgtagctccatgttggaagagatcatctgcgttgtcatttctgtatcagctgcagtcccagcacca
cagcaatagacatttggagctatatagtggatctttgagcagttcttatcagcaacaatcattccttctgttgctcttgtgtcagctccaagtataactc
catccttaaacactattccagcaattgtggtccctgttttgcgagctgcgggtagcttgtgccctttctgagtaaattcattggtcaag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: