XB-CLONE-3352347
Clone Name: IMAGE:7757605
|
Gene:
pdcd5
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DR875390 [+]
ttttttttaacaacacaaaatgtaacctttattgtctttgcatgtaatggcacataatccttacggagtcaaatatgtttttaaatgtatgtcacattca
cacattctcagaaaaacaaatctaaaacagttcctgaatttttataacaaaagtggaaaattatgtatgctgttcaaataatttaacatataaacggttt
gataaccttacacgaggaccttttaaaagaaggtccgcttgctttctttttgcttaccaaaaaatgttagatttagaaaaaggcaatgtctttttcagtc
atttttgtaacgtaaaggatttcagtgcctgtcttaaaatgcatgcagtgcttgaaagctcagtcacatcaaaggcctcgtgtttaacatctcagtaatt
gattattcagtcatcgtcttcatctgaatccatcacttttcttctgttaaactttactgtagtcttcttttctgtctgctgactcactttttccaaaatt
tctattaatccttcctctgataatttgccaccaagctgtccaaatcttgccatttgtataagataattctcaacagctttggctttttcaggtttaacga
gtgccagattattcaacctagcccgtgctgcctggctgagaacttgagctaaaatgttatttctcatgtcatcttctctttgtttggcttcttgttgaga
ctgatcatttacagcatccccatgtttactctgcagctctgccatgcgctgcctgcgaatagcctccagctccgggtccgccattgcgctccggtcctg
3' EST: DR875389 [+]ggcgagaggcaggaccggagcgcaatggcggacccggagctggaggctattcgcaggcagcgcatggcagagctgcagagtaaacatggggatgctgtaa
atgatcagtctcaacaagaagccaaacaaagagaagatgacatgagaaataacattttagctcaagttctcagccaggcagcacgggctaggttgaataa
tctggcactcgttaaacctgaaaaagccaaagctgttgagaattatcttatacaaatggcaagatttggacagcttggtggcaaattatcagaggaagga
ttaatagaaattttggaaaaagtgagtcagcagacagaaaagaagactacagtaaagtttaacagaagaaaagtgatggattcagatgaagacgatgact
gaataatcaattactgagatgttaaacacgaggcctttgatgtgactgagctttcaagcactgcatgcattttaagacaggcactgaaatcctttacgtt
acaaaaatgactgaaaaagacattgcctttttctaaatctaacattttttggtaagcaaaaagaaagcaagcggaccttcttttaaaaggtcctcgtgta
aggttatcaaaccgtttatatgttaaattatttgaacagcatacataattttccacttttgttataaaaattcaggaactgttttagatttgtttttctg
agaatgtgtgaatgtgacatacatttaaaaacatatttgactccgtaaggattatgtgccattacatgcaaagacaataaaggttacattttgtgttggt
aaaaaaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: