XB-CLONE-3352046
Clone Name: IMAGE:7757631
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Gene:
mvd
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR875589 [+]
acagcaggtgtctgggaatttccggggacaagaaatgaagaaagtgacgtgcactgcacctgttaacatagctgtcatcaaatactggggtaaacgaaac
gaggaacttattttgcctataaactcctccttaagtgtcacactgcaccaagatcagttaaaaacaacaacatctgctgctgcaagtcgggagtttacag
aagaccggatttggttgaatggcaaggaagagaacatcagccaccctcgtttgcagtcctgtctacgtgagattcgtcgtctagcaagaaaaaggaggaa
tgaagaaggtgatgaaaatgtctcaaggatcttaaatgacaaggttcacatatgttcagtcaataattttcccacagcagctggactggcatcatctgca
gctggatatgcttgccttgtgtacaccctggcaaagctttatggagttgaaggagagctgtcagagattgcacggcaggggtctggaagtgcctgcagga
gcatgtatgggggatttgtgcagtgggtaatgggagaaagggatgatgggaaagacagcttagccaaacaagtggaacctgaaagtcactggccggagct
acgagttctaatactagtggctactgcagagaagaagcatgtaggcagcacagctggtatgcagacaagtgtggagactagtccattgcttaagcttaga
gcagacttagttgtgcctgaacggatggaggctatgatcgagtcaatcagaaaaaaggattttaaggcttttggcgaaactgacatgaaggacagcaatc
agttccatgccacctgtctggacacatatcctcctatcttttaccttaacagtgtgtctcagcgtgtcattag
3' EST: DR875588 [+]ccttgcacgtaatttttattaatcattaactggcatattctgtaatgacaaaacatgaggtaatctgccaatacacaattttacaaaattttcaactatg
atattaatatctataaaattacaaacctcaatagtcaaaacatgttttaaactaagtcatgctggggtgaagcaaaaaaacaaaacaaaaaacaaacctt
attacaagtgtcagtataatgcaataccataaaagggttggaaatgtattgaattctttataacagtgatccccaaccccttgaatgttgctcccagttg
cctcaaagcaagttttggttgcataaaaaccaggtgtacttccaaatagagccttctgtagtctgacaatccacaaaaatagccagtaacagccccacgg
ggacttttattctgcttgcgttacttccaaactctttttacatttgaatgtggcttatgggtaaaaatagttgaagacccctgtttaaaagaataagtaa
acacccttttttaaaatcgctaaaattactgtgcacagtccccagaacaacacaccctcctccctgcattcagacccattctgtttctctagtacaaaca
gcaggaattacttccttgtctagtatgaagctctgtccactcttgccctccgagcactccttctctctctcacaatgatgacctgaaagaggtgcccata
gagtctgcctaattgattttaattggaacaagagcagtgagcattcccagtaggcacctctttgaggtcatcacagcaaaaaaggggagtgcccagaaag
caaaacagacagagcctcactctaattcatgaagtaact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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