XB-CLONE-3352016
Clone Name: IMAGE:7757729
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Gene:
spop
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR875609 [+]
ttcatcaattaccatgcctctgatgtcatggaaacctctggatggaagtctatggtggtctctcacccacacctggtagcagaagcataccgctcgctag
catccgcccagtgcccctttctgggccctccacgcaaacgcctgaagcaatcctaaagctgcttagtggatagcccaacgtttctcctggagccactcta
cttttcctcaaagcatgagagacattggtgggggagactgattggaggttcctgtctttacctcctccacattgtgtgatttgccaccctactccccatg
ccgcgttggctggactgagcctcctctctcccatatttctgagtgaggagcaggggccatagtggatgtgccctgaactgcggaacaaaattttatattt
tatggatggccggtgtgtttaagactgggactttcctgttatttatagggacataaagcagctttaattagccagttgcattttttctgtcccaaaggac
attggagtagagaaatttaagggctgagcaatttccgcagctctcctcatcttcccccccgcccccaaaaaggacagagttggaggcgggcacagtttat
accatttactctgggatgttttacttttttttgatatgcctccttcaaagtttgttgttaatccttccacgttcttttacttctcctgtccatcttgcct
tttagttcttacagatatccctaagcctaaaggtccttcactgatgcttcaaagccgttagatttattttgattttcgagaatataataattcctatttt
gtaaaaaaaaaaaaaaaaaaaaa
3' EST: DR875608 [+]acaaaataggaattattatattctcgaaaatcaaaataaatctaacggctttgaagcatcagtgaaggacctttaggcttagggatatctgtaagaacta
aaaggcaagatggacaggagaagtaaaagaacgtggaaggattaacaacaaactttgaaggaggcatatcaaaaaaaagtaaaacatcccagagtaaatg
gtataaactgtgcccgcctccaactctgtcctttttgggggcgggggggaagatgaggagagctgcggaaattgctcagcccttaaatttctctactcca
atgtcctttgggacagaaaaaatgcaactggctaattaaagctgctttatgtccctataaataacaggaaagtcccagtcttaaacacaccggccatcca
taaaatataaaattttgttccgcagttcagggcacatccactatggcccctgctcctcactcagaaatatgggagagaggaggctcagtccagccaacgc
ggcatggggagtagggtggcaaatcacacaatgtggaggaggtaaagacaggaacctccaatcagtctcccccaccaatgtctctcatgctttgaggaaa
agtagagtggctccaggagaaacgttgggctatccactaagcagctttaggattgcttcaggcgtttgcgtggagggcccagaaaggggcactgggcgga
tgctagcgagcggtatgcttctgctaccaggtgtgggtgagagaccaccatagacttccatccagaggtttccatgacatcagaggcatggtaattgatg
aagtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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