XB-CLONE-3351835
Clone Name: IMAGE:7757698
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Gene:
atp2a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR875729 [+]
tccatcgattcgtttgagtctcctgtacccatgaccatggctctgtctgtgctggtcaccattgaaatgtgcaatgccctcaacagtctgtcagagaacc
agtctctggtcaggatgcccccatgggtgaacatctggctgttgggctctatctgcttgtccatgtccctccacttccttatcctctatgttgagccact
tccaatgatcttcaagttgactcctctgaacttcactcagtggtttgttgtcctgaagatttccatccctgtcatcctgttggatgaactgttgaaattt
gttgctcgtaactacctggaagcataatctttaccactccaccaccttgttcctgcctaagagaaaaaaagtaaagtaaagggaactcaactttgtactg
attacaaaaatcagatctcagcccaaatcctcatttttacttttctgcccccccaataactcatcttcttctacttcatcactgccaaccctttcccccc
aacgccccgctccagagccttccaacttgggcgtctcccccaaatctgccccccaagggtgatggaattgtcgggctgttgttctgttagccaagtgcct
taatcttccattcacgaccctttttataaagcgattcaccccccccccccataaccgcaccaatatactgtacatatagaaccggtctccccaatccgga
catctcagagctaaatcatagtcgatctcggcgagag
3' EST: DR875728 [+]ttttttgagagtttaaattattctttaattttaataaatagctataagagtttcctcgtagatttctcgcgtccgtatctctgctacaagcggcaactcc
ctcgcggggctggagagggatcaacaacgccacactgcactttaacaccgaccgtgcgttcttctctctcgccgagatcgactatgatttagctctgaga
tgtccggattggggagaccggttctatatgtacagtatattggtgcggttatggggggggggggtgaatcgctttataaaaagggtcgtgaatggaagat
taaggcacttggctaacagaacaacagcccgacaattccatcacccttggggggcagatttgggggagacgcccaagttggaaggctctggagcggggcg
ttggggggaaagggttggcagtgatgaagtagaagaagatgagttattgggggggcagaaaagtaaaaatgaggatttgggctgagatctgatttttgta
atcagtacaaagttgagttccctttactttactttttttctcttaggcaggaacaaggtggtggagtggtaaagattatgcttccaggtagttacgagca
acaaatttcaacagttcatccaacaggatgacagggatggaaatcttcaggacaacaaaccactgagtgaagttcagaggagtcaacttgaagatcattg
gaagtggctcaacatagaggataaggaagtggagggacatggacaagcagatagagcccaacagccagatgttcacccctgggggcatcctgaccagaga
ctggttctctgacagactgttgaggggcatgcacatttcaatggtga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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