XB-CLONE-3351773
Clone Name: IMAGE:7757560
|
Gene:
atp2a1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DR875770 [+]
cttgctctggctacagggacaccccacccaaaagggaagagatggtgctcgatgacgccaccaagtttgttgactatgagactgatctcaccttcgttgg
ctgacaggcaaggatggaagccgcacacaccaagaccaccgaggaatgcttggcctattttggggtcaacgagaacactggtctctctcctgagatagtc
aagaagaacttcgacaagttcggacccaatgaacttccagctgaggaaggaaagtccatatgggaactggtggcagaacagtttgaagatttactggtcc
ggattctgctgctggccgcctgtatatcattcgtgctggcctggtttgaagaaggtgaagagaccgtcacagcgtttgttgaaccattcgtcattctcct
tattctcattgccaatgcggttgtcggtgtctggcaggaaagaaacgcagaagatgccattgaagccctcaaagaatatgaacctgagatgggcaaagtg
taccgtagcgacagaaagtctgtgcagagaatcaaggccagagaaatcgtgcccggagacatcgtggaagtggctgttggtgacaaagtcccagcagata
tcagactcatcagcattaagtccactaccctgcgtattgaccagtccatcctgacaggagagtctgtctctgtcatcaagcacaccgaagctgtccctga
ccccagagctgtcaaccaggataagaagaacatgctgttctctggtaccaatgttggagctggcaaggccattggtgttgtcattgccacnagaccccat
acagaaattggcaagatccgtgatgagatggcggcaacagaacaggaaaagacccccctgcagcagaaactggatg
3' EST: DR875769 [+]aggttttttttgagagtttaaattattctttaattttaataaatagctataagagtttcctcgtagatttctcgcgtccgtatctctgctacaagcggca
actccctcgcggggctggagagggatcaacaacgccacactgcactttaacaccgaccgtgcgttcttctctctcgccgagatcgactatgatttagctc
tgagatgtccggattggggagaccggttctatatgtacagtatattggtgcggttatgggggggggggggtgaatcgctttataaaaagggtcgtgaatg
gaagattaaggcacttggctaacagaacaacagcccgacaattccatcacccttggggggcagatttgggagacgcccaagttggaaggctctggagcgg
ggcgttggggggaaagggttggcagtgatgaagtagaagaagatgagttattgggggggcagaaaagtaaaaatgaggatttgggctgagatctgatttt
tgtaatcagtacaaagttgagttccctttactttactttttttcttccaggtagttacgagcaacaaatttcaacagttcatccaacaggatgacaggga
tggaaatcttcaggacaacaaaccactgagtgaagttcagaggagtcaacttgatggctggagtaagtggaacgcgggttgtgcccacacgtacgtagtt
gcacctgtcaatgacaccctctggggcacccttcacaaacatcttgttgccaacggcagcgcgggaagctttggctggggtgcagtagacggacattgac
ttcctgtcacgggagaact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: