XB-CLONE-3351418
Clone Name: IMAGE:7757540
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Gene:
ptp4a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR875977 [+]
tgcttctctgccctagatgtaagctcccattggtcacagtaaggttaaatgcttgaggtttggctagtctgtatttcctactgatatcaaaacaatgctg
tttgtattgcctgcacaaacttcacttgtcagatagatgcaggtgtttagtagaacactacagtttactttttaattcatactggcaaactataggcaag
taaaatgttatatattttaaaggctgattgtaaacggcaaaataagggaggtcaagaaattctaagggaagtggaactaatcagctgaaactgataaggt
attccatcctgtgctaaaacataaacaggagccccaacaaaataaatagacatgaacaagtgtatctttccctaaaatacagatttcactattacaatgg
aggggttctctaacaaatacatgtcttacatcttcctccagttggccagaggatatgcacacagtagctctgattatgcaaaagcaagaatttatctcct
tctggcagctgaaagttttgtttccctgcctccatacagatggtccatgcttagatatgtttaaaggacacagtattttgtcaaaacagttaattttcca
ggaggaacagatctaaacaaactgtagttctgtctattttcatgcacacgtatggcattgctgttaccaagaccaaaaaagggatcattgacagattcta
ttagaagttacaaattctatttcatttatatccaaaactggatcatttggtggccactgaatgacagatttggcttaaggctgaacagcaatcgttctat
ttcagact
3' EST: DR875976 [+]cgagaggccgggcacgaacagccgggattcaatgaacagctcgcccctgactgtttcctcttggagtggacgtggcatatttatggcgattcggcgcata
agaacagaatactgccaggctcagcacggctttggcgcggacaagtgagctgaaaagtctgcctctttgagcccaccctccaatcatcagccttttgcca
gctgcagtaaagcatatgggagttactatttattttcataccttgagaccatcttgcttttgcaaattaatttttccttttgtgaagggaagacttttac
aaagtggggactaagtgcggaggccatctctgcagttattttttcttttccaaatgagtgcctccttgccataaagaccattggtccatttttttttttt
taatttctttttcatttcccttccataagttatgtaagaaaattccattggatgctgtactgcatccctcacaaagtgcttgattgcattaaaaccatcg
ctgggacttttgcaataagtcttttttttttttttcttcctgcaaaaaaatttttttttttctttatagaaatttttaatattttttgctgtgaattttt
caggattttttcccaagaaatatccttatttttttaatctgatcaaaatgaaccgtccagctccagtagaaatctctcatgagtgcatgcgttttcttat
tacccataatccaaccaatgccacactcaacaagttcacagaggagctaaagaaatatggagttaccacccttgtgagagtatgcgatgcaacatatgat
aaagcaccggtagaaaaggaagggatacaggttttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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