XB-CLONE-3341892
Clone Name: IMAGE:7762332
|
Gene:
nudcd2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DR884370 [+]
ctcacttcgatgagaggagtggggtggtcccctgtcaaacaccatggggttgctggtatcagaccatggaagaggtgttcattgaagtcaaagtacccga
tgggacccttgccaaagaagtccagtgcaaattgggaagtcgggatatttcactggttgtgaagggaaaggacatacttaaggggaagttgtttgattct
acaatatccgacgaagcaacatggactctagaggataggaaactaataaggataatcctaacaaaaacaaatcgggatgctggcaattgctggacctctt
tatcagaaggggactattccgcagacccatggatccaggatgagatgcaaaagaagcttacactggaaagatttcagagagagaatccaggcttcgattt
cagcggggcagaaatatcagggaactacagcaaagggggaccagacttctcaaatctacaaaagtgaaggagagagacgaaaagccagcattatttatgt
aggataattccagggatctgatccctaaagatatcattggttgtttataagtaattttgccaaagtcagttacaaaacttttattattttctgtattttc
tatggcaccataagtctttgccattagccaactgaatgcactcttcagcaaaatggctttttttaatattattaaaaaagattgaatgtgcccttttttt
cttgctcatgtttactgctaatctctgctccttattactccctgtagcctataggtacattaaatacattaaagaaaggagaaagcactagaaggccagc
aataccagctttgccatttactttccctacatgagtgctattcagcacacttatgcaaagtgatatgtaatgtcatcagcgctgactttagactttacca
agtaagaatgggg
3' EST: DR884369 [+]caaatgtatttatttaaacaagaaaccccttgaaaccaagaaaagaacattgataagcactcttgtttaaaatatttttatcactcttgttaactaaagt
ttcttttatttggggcaactgaaccagtatttcttatcctttattcaccaagatgtcaatcccacgctaaccctttacaaaacatcacaatcagggtttg
ctttttctacagatcttacagaaaatgtaataactgtaatacagtgctggttagactttgcttagcctccatgtagaggtagcagctgctcctgctttgt
acagggaacaagaagtccattcatgctcatcgatttgcataggattctcagatgcagagggggaaacattgctccagacagcctacagattctattcctt
gtgcactgtgtacagatagtgcactattaattctggcacataatcacccaccttcctcccaagtgaattgatgtgtcttataaacacaaagtgttcccgt
gtgtatttttcagactgcagataacctagattattttaaataaattttctgcctgcagaggactgcaactatatttctttaactgggatacaggcttcag
caatcagacttcccctgctacacaacttttagccgcctcttccttgcttcactattcagcccagcagtctataatgcagcactgatgctcccatgggaag
cacagcctcttccaccccattccttacttggtaaagtctaaagtcagcgctgatgacattacatatcactttgcataagtgtgctgaatagcactcatgt
agggaaagtaaatggcaaagctggtattgctggccttctagtgctttctcctttctttaatgtatttaatgtacctatangctacagggagtaataagga
gc
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1364 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: